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    • Kit yo Gusuzuma Impinduka za Gene 29 z'Umuntu (Fluorescence PCR)-RUO

    Ishusho ya Human EGFR Gene 29 Mutations Detection Kit (Fluorescence PCR)-RUO
    • Kit yo Gusuzuma Impinduka za Gene 29 z'Umuntu (Fluorescence PCR)-RUO

    Ibisobanuro bigufi:

    [Kode y'ibicuruzwa] HWTS- TM4008

    Ibisobanuro bigufi

    Iyi kit ikoreshwa mu gupima mu buryo bw’umwimerere impinduka zisanzwe muri exons 18-21 za gene ya EGFR mu bipimo byatanzwe n’abarwayi ba kanseri y’ibihaha batari abarwayi b’uturemangingo duto (reba Imbonerahamwe ya 1 kugira ngo umenye ibisobanuro birambuye).

    Ikoreshwa ry'ubushakashatsi gusa


    Ibisobanuro birambuye ku gicuruzwa

    Ibirango by'ibicuruzwa

    Izina ry'igicuruzwa

    Kit yo Gusuzuma Impinduka za Gene 29 z'Umuntu (Fluorescence PCR)-RUO

    Ubumenyi bw'indwara

    Kanseri y'ibihaha yabaye intandaro ikomeye y'impfu za kanseri ku isi, ibangamira cyane ubuzima bw'abantu. Kanseri y'ibihaha itari iy'uturemangingo duto ifata abagera kuri 80%-85% by'abarwayi ba kanseri y'ibihaha. EGFR ni yo ntego ikomeye mu kuvura kanseri y'ibihaha itari iy'uturemangingo duto. Fosforili ya EGFR ishobora guteza imbere gukura kw'uturemangingo twa kanseri, gutandukanya, kwinjira, kwangirika, kurwanya apoptosis, no guteza imbere angiogenesis ya tumor. EGFR tyrosine kinase inhibitors (TKI) ishobora guhagarika inzira y'ibimenyetso bya EGFR ibuza EGFR autophosphorylation, bityo ikabuza ikwirakwira no gutandukana kw'uturemangingo twa kanseri, iteza imbere apoptosis ya tumor cell, ikagabanya angiogenesis ya tumor, nibindi, kugira ngo hagerwe ku buvuzi bushingiye ku tumor [1]. Ubushakashatsi bwinshi bwagaragaje ko ubushobozi bwo kuvura bwa EGFR-TKI bufitanye isano rya bugufi n'imiterere y'impinduka za gene za EGFR, kandi bushobora kubuza by'umwihariko gukura kw'uturemangingo twa kanseri hamwe na EGFR gene mutation [2,3]. Ingirabuzimafatizo ya EGFR iherereye ku kuboko gugufi kwa chromosome 7 (7p12), ifite uburebure bwose bwa 200Kb kandi igizwe na exon 28. Agace k'impinduka kaboneka cyane cyane muri exon 18 kugeza 21, codons 746 kugeza 753 deletion mutation kuri exon 19 ingana na 45% naho L858R mutation kuri exon 21 ingana na 40% kugeza 45% [4]. Amabwiriza ya NCCN yo gusuzuma no kuvura kanseri y'ibihaha itari iy'uturemangingo duto avuga neza ko gupima EGFR mutation ari ngombwa mbere yo guhabwa EGFR-TKI. Iyi mashini ikoreshwa mu kuyobora gutanga imiti ya epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), kandi igatanga ishingiro ry'imiti yihariye ku barwayi ba kanseri y'ibihaha itari iy'uturemangingo duto. Iyi mashini ikoreshwa gusa mu kumenya impinduka zisanzwe muri gene ya EGFR ku barwayi ba kanseri y'ibihaha itari iy'uturemangingo duto. Ibisubizo by'ibizamini ni ibyo kwifashisha mu buvuzi gusa kandi ntibigomba gukoreshwa nk'ishingiro ryonyine ryo kuvura abarwayi ku giti cyabo. Abaganga bagomba gusuzuma imiterere y'umurwayi, ibimenyetso by'imiti, n'uburyo avurwa. Uburyo yabyakiriye n'ibindi bimenyetso by'ibizamini bya laboratwari n'ibindi bintu bikoreshwa mu gupima neza ibisubizo by'ibizamini.

    Umuyoboro

    Impuguke mu gusubiza Umuyoboro
    EGFR IC Reaction Buffer Umuyoboro wa FAM
    L858R Reaction Buffer Umuyoboro wa FAM
    19del Reaction Buffer Umuyoboro wa FAM
    T790M Reaction Buffer Umuyoboro wa FAM
    G719X Reaction Buffer Umuyoboro wa FAM
    3Ins20 Reaction Buffer Umuyoboro wa FAM
    L861Q Reaction Buffer Umuyoboro wa FAM
    S768I Reaction Buffer Umuyoboro wa FAM

    Ibipimo bya tekiniki

    Ububiko Amazi: ≤ -18℃ Mu mwijima
    Igihe cyo kumara Amezi 12
    Ubwoko bw'ingero inyama nshya y'ikibyimba, igice cyakonjeshejwe cy'indwara, igice cyangwa igice cy'indwara cyashyizwemo parafini, plasma cyangwa serum
    CV <5.0%
    LoD Gupima umuti w’impinduka za aside nucleic munsi ya 3ng/μL wild-type, bishobora kumenya neza igipimo cya 1% cy’impinduka;

    b) reaction ya aside nucleic Munsi y'igipimo cya 1% cy'impinduka, 1 × 103Copies / mL mutation ishobora kugaragara neza mu ibara ry'inyuma rya 1 × 105Copies / mL;

    c) Igisubizo cy’inyigo y’imbere gishobora kumenya umusaruro w’inyigo SW3 ufite umupaka wo hasi cyane w’inyigo y’imbere y’ikigo. Iyo agakoresho kabonye ibikoresho by’inyigo by’igihugu, ibikoresho by’inyigo by’inyigo bya 2.5% by’impinduka mu mihindagurikire y’impinduka mu miterere y’uturemangingo hamwe n’impinduka mu mibare ya EGFR gene amplification hamwe n’impinduka mu mibare ya kopi ya 4 bishobora kumenyekana neza.
    Ubwihariye Nta buryo bwo guhuza ADN y'umuntu yo mu bwoko bwa wild-type hamwe n'ubundi bwoko bwa mutant
    Ibikoresho Bikoreshwa Porogaramu za mudasobwa zikoreshwa mu buryo bwa "Real-Time PCR Systems",

    Sisitemu za PCR zikoreshwa mu gihe nyacyo 7300,

    Sisitemu 5 za PCR za QuantStudio® mu gihe nyacyo,

    Sisitemu ya LightCycler® 480 ya PCR mu gihe nyacyo,

    Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo.

     

    Urugendo rw'akazi

    Kit yo Gusuzuma Impinduka za Gene 29 z'Umuntu (Fluorescence PCR)-RUO (2)

     

    Reagents zirakenewe ariko ntizitangwa:Amazi ya DNase/RNase Free, ethanol idakoresha amazi. Mu gihe ugerageza icyitegererezo cy’uturemangingo twa parafini, ni byiza gukoresha QIAamp DNA FFPE Tissue Kit (56404) na QIAGEN na Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) na Tiangen Biotech (Beijing) Co., Ltd.. Mu gihe ugerageza icyitegererezo cya plasma/serum, ni byiza gukoresha Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. cyangwa QIAamp Circulating Nucleic Acid Kit (55114) na QIAGEN.

    Ibikoresho bikenewe ariko ntibitangwa: Amabwiriza y'ubuntu ya DNase/RNase, uturindantoki dukoreshwa gasangwa, umuyoboro wa DNase/RNase Free Centrifuge, uduce tw'imiyoboro 8 twa PCR, centrifuge.

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