Agakoresho ko gupima aside ya nucleic ya Dengue yumye mu buryo bukonje (Fluorescence PCR)-RUO
Izina ry'igicuruzwa
Agakoresho ko gupima aside ya nucleic ya Dengue yumye mu buryo bukonje (Fluorescence PCR)-RUO
Ubumenyi bw'indwara
Indwara ya Dengue (DF), iterwa n'ubwandu bwa Denguevirus (DENV), ni imwe mu ndwara zandura za arbovirus zikunze kugaragara cyane. Uburyo bwo kuyikwirakwiza burimo Aedes aegypti na Aedes albopictus. Indwara ya Dengue ikunze kugaragara cyane mu turere dushyuha n'utwo mu turere dushyuha. Indwara ya Dengue ikomoka kuri flavivirus iri munsi ya flaviviridae, kandi ishobora gushyirwa mu byiciro 4 hakurikijwe antigen yo hejuru. Ibimenyetso by'ubwandu bwa DENV birimo kuribwa umutwe, umuriro, intege nke, kwiyongera kwa lymph node, leukopenia n'ibindi, no kuva amaraso, gutungurwa, gukomereka mu mwijima cyangwa ndetse no gupfa mu bihe bikomeye. Mu myaka ya vuba aha, imihindagurikire y'ikirere, iterambere ryihuse ry'imijyi, iterambere ryihuse ry'ubukerarugendo n'ibindi bintu byatanze uburyo bwihuse kandi bworoshye bwo korohereza no gukwirakwiza DF, bigatuma agace k'icyorezo cya DF karushaho kwaguka. Kubwibyo, gushyiraho uburyo bworoshye, bwihariye kandi bwihuse bwo gusuzuma DENV bufite akamaro kanini mu gusuzuma DF.
Ibipimo bya tekiniki
| Ububiko | 2-8℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | serum |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | Kopi 500/mL |
| Ubwihariye | Ibizamini ntibigaragaza ko ari bibi ku bipimo bya serum bya virusi ya dengue; ibisubizo by'ibizamini by'ihungabana bigaragaza ko iyo igipimo cya bilirubin mu maraso kitarenze 168.2μmol/mL, igipimo cya hemoglobine ikorwa na hemolysis kitarenze 130g/L, igipimo cya lipide mu maraso kitarenze 65mmol/mL, kandi igipimo cyose cya IgG mu maraso kitarenze 5mg/mL, nta ngaruka bigira ku kumenyekana kwa virusi ya dengue; koresha virusi ya Hepatite A, virusi ya Hepatite B, virusi ya Hepatite C, virusi ya Herpes, virusi ya Eastern equine encephalitis, virusi ya Hantaan, virusi ya Bunia, virusi ya West Nile, virusi ya Zika n'ibipimo bya serum ya genome y'abantu kugira ngo hakoreshwe ikizamini cya cross-reactivity, kandi ibisubizo bigaragaza ko nta mpungenge hagati y'iki gikoresho n'udukoko twanduza twavuzwe haruguru. |
| Ibikoresho Bikoreshwa | Ubwoko bwa I: Porogaramu za Biosystems 7500 za Mudasobwa za PCR, Sisitemu za QuantStudio®5 za Mudasobwa za PCR, Sisitemu za SLAN-96P za Mudasobwa za PCR (Hongshi Medical Technology Co., Ltd.), Sisitemu yo Gupima PCR ya LineGene 9600 Plus (FQD-96A, ikoranabuhanga rya Hangzhou Bioer), Umuyoboro w'amashanyarazi wa MA-6000 (Suzhou Molarray Co., Ltd.), Sisitemu ya BioRad CFX96 ya Mudasobwa za PCR, Sisitemu ya BioRad CFX Opus 96 ya Mudasobwa za PCR. Ubwoko bwa kabiri: EudemonTMAIO800 (HWTS-EQ007) yakozwe na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
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Urugendo rw'akazi
Ibikoresho bitanga umusaruro bigomba ariko ntibitangwa:
Ubwoko bwa I: Macro & Micro-Test General ADN/RNA Kit (HWTS-3017) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral ADN/RNA Column (HWTS-3022).
Ubwoko bwa II: Macro & Micro-Test General ADN/RNA Kit (HWTS-3023-8), ishobora gukoreshwa hamwe na EudemonTM AIO800 (HWTS-EQ007) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Mbere yo gukoresha Type II test reagent, umukiriya afite igikoresho gishyigikira gukuramo ibintu n'igikoresho cyo kongera imbaraga, kandi injeniyeri azayobora umukiriya gukora igikorwa cyo kugerageza iyo agikoresheje bwa mbere.
Ibikoresho bikoreshwa bigomba ariko ntibitangwa:Amabwiriza y'ubuntu ya DNase/RNase, uturindantoki dukoreshwa rimwe, imiyoboro ya DNase/RNase ya EP, centrifuge, icyuma gihagararamo magnetic, uturongo tw'imiyoboro 8 twa PCR.
