Agakoresho ko Gupima Aside ya Leukocyte B27 (Fluorescence PCR)-RUO

Ibisobanuro bigufi:

Agakoresho ko Gupima Aside ya Leukocyte B27 mu Mutwe w'Abantu (Fluorescence PCR)

HWTS-GE011A

Iyi kit ikoreshwa mu gupima ADN mu bwoko bwa antigen ya leukocyte y'umuntu nka HLA-B * 2702, HLA-B * 2704 na HLA-B * 2705.

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Agakoresho ko Gupima Aside ya Leukocyte B27 mu Mutwe w'Abantu (Fluorescence PCR)

Ubumenyi bw'indwara

Ankylosing spondylitis (AS) ni indwara idakira itera ububabare ikwirakwira cyane cyane mu ruti rw'umugongo kandi ishobora no gufata ingingo za sacroiliac n'izindi ngingo ku rugero rutandukanye. Byagaragaye ko AS igaragaza ubwiyongere bw'imiryango kandi ifitanye isano rya hafi na antigen ya leukocyte y'umuntu HLA-B27. Ku bantu, ubwoko burenga 70 bwa HLA-B27 bwavumbuwe kandi bwaramenyekanye, muri bwo, HLA-B*2702, HLA-B*2704 na HLA-B*2705 ni zo ndwara zikunze kugaragara cyane. Mu Bushinwa, Singapuru, Ubuyapani n'akarere ka Tayiwani mu Bushinwa, ubwoko bukunze kugaragara bwa HLA-B27 ni HLA-B*2704, bungana na 54%, bugakurikirwa na HLA-B*2705, bungana na 41%. Iyi kit ishobora kubona ADN mu byiciro bya HLA-B*2702, HLA-B*2704 na HLA-B*2705, ariko ntabwo ibitandukanya.

Umuyoboro

FAM	HLA-B27
VIC/HEX	Igenzura ry'imbere

Ibipimo bya tekiniki

Ububiko	Amazi: ≤-18℃ Mu mwijima
Igihe cyo kumara	Amazi: Amezi 18
Ubwoko bw'ingero	ingero z'amaraso yose
Ct	≤40
CV	≤5.0%
LoD	1ng/μL
Ubwihariye	Ibisubizo by'ibizamini byabonetse muri aka gakoresho ntibizakorwaho na hemoglobine (<800g/L), bilirubini (<700μmol/L), na lipide mu maraso/triglycerides (<7mmol/L) mu maraso.
Ibikoresho Bikoreshwa	Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500Sisitemu za Biosystems zikoreshwa StepOne Real-Time PCR Systems Sisitemu ya LightCycler®480 Real-Time PCR Sisitemu ya Agilent-Stratagene Mx3000P Q-PCR