Virusi ya Zika
Izina ry'igicuruzwa
HWTS-FE002 Kit yo Gupima Aside Nucleic ya Zika Virus (Fluorescence PCR)
Icyemezo
CE
Ubumenyi bw'indwara
Virusi ya Zika iri mu bwoko bwa Flaviviridae, ni virusi ya RNA ifite umugozi umwe ifite umurambararo wa 40-70nm. Ifite agace gato, irimo nucleotides 10794, kandi ikubiyemo aside amine 3419. Dukurikije ubwoko bw'uturemangingo, igabanyijemo ubwoko bwa Afurika n'ubwoko bwa Aziya. Indwara ya virusi ya Zika ni indwara yandura yihuta iterwa na virusi ya Zika, yandurira ahanini mu kurumwa n'umubu wa Aedes aegypti. Ibiranga indwara ni umuriro, ibisebe, arthralgia cyangwa conjunctivitis, kandi ntabwo yica. Dukurikije Ishami ry'Umuryango w'Abibumbye ryita ku Buzima, microcephaly y'abana bato na syndrome ya Guillain-Barre (syndrome ya Guillain-Barré) bishobora kuba bifitanye isano n'ubwandu bwa virusi ya Zika.
Umuyoboro
| FAM | Aside y'ingirabuzimafatizo ya virusi ya Zika |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤30℃ kandi birinzwe n'urumuri |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | serumu nshya |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Kopi 500/mL |
| Ubwihariye | Koresha agakoresho kugira ngo umenye ingero za serum zifite virusi ya Zika, kandi ibisubizo ni bibi. Ibisubizo by'ibizamini by'ihungabana bigaragaza ko iyo ubwinshi bwa bilirubin mu maraso butarenze 168.2μmol/ml, ubwinshi bwa hemoglobine bukorwa na hemolysis butarenze 130g/L, ubwinshi bwa lipide mu maraso butarenze 65mmol/ml, ubwinshi bwa IgG mu maraso butarenze 5mg/mL, nta ngaruka bigira ku virusi ya dengue, virusi ya Zika cyangwa virusi ya chikungunya. Virusi ya Hepatite A, virusi ya Hepatite B, virusi ya Hepatite C, virusi ya Herpes, virusi ya Eastern equine encephalitis, virusi ya Hantavirusi, virusi ya Bunya, virusi ya West Nile n'ingero za serum ya genome y'abantu byatoranijwe kugira ngo bipimwe ku buryo bwo guhuza, kandi ibisubizo bigaragaza ko nta bwinshi bwa filime hagati y'iki gikoresho n'udukoko twavuzwe haruguru. |
| Ibikoresho Bikoreshwa | Sisitemu za PCR za ABI 7500 mu gihe nyacyoSisitemu za PCR za ABI 7500 zihuta cyane mu gihe nyacyo QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Amahitamo ya 1.
Kit nto ya QIAamp Viral RNA (52904), Ikoreshwa rya aside ya nucleic cyangwa isuku (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd.Icukurwabigomba gukurwamo hakurikijwe amabwiriza yo gukuramo, kandi ingano y’ibicuruzwa byatanzwe ni 140 μL naho ingano y’ibicuruzwa byatanzwe ni 60 μL.
Amahitamo ya 2.
Kit ya Macro & Micro-Test Viral ADN/RNA (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ikurwamo rigomba gukurwamo hakurikijwe amabwiriza. Ingano y'icyitegererezo cyo gukuramo ni 200 μL, naho ingano ya elution isabwa ni 80μL.
