Virusi ya Zika
Izina ryibicuruzwa
HWTS-FE002 Zika Virus Nucleic Acide Detection Kit (Fluorescence PCR)
Icyemezo
CE
Epidemiologiya
Virusi ya Zika ni iy'ubwoko bwa Flaviviridae, ni virusi imwe ya virusi ya RNA ifite umurongo wa diameter 40-70nm. Ifite ibahasha, irimo nucleotide 10794, kandi ikubiyemo aside amine 3419. Ukurikije genotype, igabanijwe mubwoko bwa Afrika nubwoko bwa Aziya. Indwara ya virusi ya Zika ni indwara yonyine yanduza indwara yanduye iterwa na virusi ya Zika, yandurira cyane cyane mu kurumwa n'umubu wa Aedes aegypti. Ibiranga ivuriro ahanini ni umuriro, guhubuka, arthralgia cyangwa conjunctivitis, kandi ni gake byica. Ishami ry'Umuryango w'Abibumbye ryita ku Buzima rivuga ko microcephaly ya neonatal na syndrome ya Guillain-Barre (syndrome ya Guillain-Barré) ishobora kuba ifitanye isano no kwandura virusi ya Zika.
Umuyoboro
| FAM | Acide nucleic aside |
| ROX | Igenzura ryimbere |
Ibipimo bya tekiniki
| Ububiko | ≤30 ℃ & ikingiwe urumuri |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | serumu nshya |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500Copi / mL |
| Umwihariko | Koresha ibikoresho kugirango umenye serumu hamwe na virusi ya Zika mbi, kandi ibisubizo nibibi. Ibisubizo by’ibizamini byerekana ko iyo bilirubine iri muri serumu itarenze 168.2μmol / ml, intungamubiri ya hemoglobine ikorwa na hemolysis ntirenza 130g / L, ubwinshi bw’amaraso ya lipide ntabwo burenga 65mmol / ml, igiteranyo cya IgG muri serumu ntikirenza 5mg / mL, nta ngaruka za virusi ya dengue, Zika. Virusi ya Hepatitis A, virusi ya Hepatitis B, virusi ya Hepatite C, virusi ya Herpes, virusi ya encephalitis yo mu burasirazuba, virusi ya Hantavirus, Bunya, virusi ya West Nile na virusi ya genomic serumu y’abantu yatoranijwe kugira ngo isuzumwe n’ibisubizo, kandi ibisubizo byerekana ko nta reaction y’umusaraba iri hagati y’iki gitabo na virusi zavuzwe haruguru. |
| Ibikoresho bikoreshwa | ABI 7500 Sisitemu nyayo-PCRABI 7500 Byihuse-Igihe Cyuzuye PCR QuantStudio®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Ihitamo 1.
QIAamp Virus RNA Mini Kit (52904), Gukuramo Acide Nucleic cyangwa Reagent yo kweza (YDP315-R) na Tiangen Biotech (Beijing) Co, Ltd.Gukuramobigomba gukururwa ukurikije amabwiriza yo gukuramo, kandi ingano yo gukuramo ni 140 μL naho ingano yo gusabwa ni 60 μL.
Icya 2.
Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acide (HWTS-3006). Gukuramo bigomba gukururwa ukurikije amabwiriza. Ingano yicyitegererezo ni 200 μL, naho ingano yo gusabwa ni 80μL.
