Zika
Izina ry'ibicuruzwa
Hwts-Fe002 Zika Virnus Nuclec Kumenya Acide Gutahura (Fluorescence PCR)
Icyemezo
CE
Epidemiology
Virusi ya Zika ni iy'ubwoko bwa Flavivilidae, ni virusi imwe ihagaze neza - yanduye RNA ifite diameter ya 40-70nm. Ifite ibahasha, ikubiyemo 10794 nucleodes, kandi ikubiyemo 3419 aside amine. Ukurikije Genotoype, igabanijwemo ubwoko bwa nyafurika nubwoko bwa Aziya. Indwara ya Zika ni indwara yo kwiyitirira cyane yatewe na virusi ya Zika, yanduzwa cyane cyane binyuze mu kurumwa n'umubu wa Aedes Aedes. Ibiranga ubuvuzi ni umuriro, guhubuka, arthraliji cyangwa conjunctivitis, kandi ni gake byica. Dukurikije umuryango w'ubuzima ku isi, uruhinja rwa Neonata na Guillacephaly na Guillani-barf-barré-barré-barré) rushobora guhuzwa na virusi ya Zika.
Umuyoboro
| Umuryango | Acide ya zika virusi |
| Rox | Kugenzura imbere |
Tekinike
| Ububiko | ≤30 ℃ & Kurinzwe kumucyo |
| Imibereho-Ubuzima | Amezi 12 |
| Ubwoko bw'ikigereranyo | serum nshya |
| Ct | ≤38 |
| CV | <5.0% |
| Lod | 500copies / ml |
| Umwihariko | Koresha ibikoresho kugirango umenye serumu yintangarugero hamwe na virusi ya ZIKA IBI, kandi ibisubizo nibibi. Ibisubizo by'ibizamini byo kwivanga byerekana ko iyo kwibanda kuri Bilirubin muri Serumu bitarenze 168. Kwibanda muri Serumu ntabwo birenze 5mg / ml, nta ngaruka kuri virusi ya dengue, virusi ya Zika cyangwa kwandura virusi ya vikungunya. Hepatite Arus, Virusi ya Hepatite B, virusi ya hepatite c virusi, virusi ya Herpefiya, Virusi ya Bunya, Ububiko bwa Nntavirus, Ububiko bwa NINYA, Ibisubizo byerekana Kwambukiranya hagati yibi bikoresho hamwe nimbaraga zavuzwe haruguru. |
| Ibikoresho bisabwa | Abi 7500 Igihe Cyigihe cya PCRAbi 7500 byihuse-sisitemu ya PCR Umutobe®5-igihe nyacyo PCR sisitemu Scan-96p sisitemu nyayo pcr sisitemu Umucyo®480 nyamara-sisitemu ya PCR Linegene 9600 wongeyeho sisitemu yigihe cya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Ihitamo rya 1.
Qiaimp viral rna mini kit (52904), acide ya nucleic yo gukuramo cyangwa kweza reagent (YDP315-R) na Tiangen biotech (Beijing) Co, Ltd.Gukuramobigomba gukurwa ukurikije amabwiriza yo gukuramo, kandi umubumbe wasabwe ni 140 μl hamwe nububiko bwikirenga busabwa ni 60 μl.
IHitamo 2.
Macro & Micro-Ikizamini cya virusi ya virusi / RNA (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na micro & micro-Ikizamini Automatic Automac (HWTS-3006). Gukuramo bigomba gukururwa ukurikije amabwiriza. Gukuramo icyitegererezo cyimibutso 200 μl, hamwe nububiko bwabigenewe ni 80μl.
