Staphylococcus Aureus na Methicillin-Irwanya Staphylococcus Aureus (MRSA / SA)

Ibisobanuro bigufi:

Iki gikoresho gikoreshwa mugushakisha ubuziranenge bwa staphylococcus aureus na methicillin irwanya staphylococcus aureus nucleic acide mu byitegererezo by'ibibyimba by'abantu, ingero zo mu mazuru hamwe n'uruhu hamwe n'indwara zanduye zanduye muri vitro.


Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-OT062 Staphylococcus Aureus na Methicillin-Resistant Staphylococcus Aureus (MRSA / SA) Nucleic Acide Detection Kit (Fluorescence PCR)

Icyemezo

CE

Epidemiologiya

Staphylococcus aureus ni imwe muri bagiteri zikomeye zitera indwara ya nosocomial.Staphylococcus aureus (SA) ni iya staphylococcus kandi ihagarariye bagiteri nziza ya Gram-positif, ishobora kubyara uburozi butandukanye hamwe na enzymes zitera.Indwara ya bagiteri ifite ibiranga gukwirakwiza kwinshi, gutera indwara zikomeye hamwe n’igipimo kinini cyo guhangana.Thermostable nuclease gene (nuc) ni gene yabitswe cyane ya staphylococcus aureus.

Umuyoboro

FAM methicilline-irwanya mecA gene
ROX

Igenzura ryimbere

CY5 staphylococcus aureus nuc gene

Ibipimo bya tekiniki

Ububiko ≤-18 ℃ & ikingiwe urumuri
Ubuzima bwa Shelf Amezi 12
Ubwoko bw'icyitegererezo spumum, uruhu hamwe nuduce twanduye twanduye, hamwe nizuru rya swab
Ct ≤36
CV ≤5.0%
LoD 1000 CFU / mL staphylococcus aureus, 1000 CFU / mL ya bacteri irwanya methicilline.Iyo igikoresho kimenye LoD yigihugu, 1000 / mL staphylococcus aureus irashobora kuboneka
Umwihariko Ikizamini cya cross-reactivite cyerekana ko iki gikoresho kidafite reaction yizindi ndwara ziterwa nubuhumekero nka methicillin-yunvikana na staphylococcus aureus, coagulase-negative staphylococcus, methicillin-anti-staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, kle mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilose, moraxella catarrhalis, neisseria meningitidis, grippe haemophilus.
Ibikoresho bikoreshwa Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR

QuantStudio®5 Sisitemu nyayo-PCR

SLAN-96P Sisitemu nyayo-PCR

Umucyo®480 Sisitemu nyayo-PCR

LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR

MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe

BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR

BioRad CFX Opus 96 Sisitemu Yigihe-PCR

Urujya n'uruza rw'akazi

Ihitamo 1.

Macro & Micro-Test Genomic ADN / RNA Kit (HWTS-3019) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. irashobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS- 3006B).Ongeramo 200µL ya saline isanzwe kumvura yatunganijwe, hanyuma intambwe ikurikiraho igomba gukururwa ukurikije amabwiriza, kandi ingano isabwa ni 80µL.

Icya 2.

Urugero rwa Macro & Micro-Ikigereranyo Cyisohoka Reagent (HWTS-3005-8) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ongeramo 1mL yumunyu usanzwe mumvura nyuma yo koza hamwe na saline isanzwe, hanyuma uvange neza.Centrifuge kuri 13,000r / min muminota 5, ikureho ndengakamere (kubika 10-20µL ya supernatant), hanyuma ukurikize amabwiriza yo gukuramo nyuma.

Gusabwa gukuramo reagent: Gukuramo Acide Nucleic Acide cyangwa Isukura Reagent (YDP302) na Tiangen Biotech (Beijing) Co, Ltd.Gukuramo bigomba gukorwa cyane ukurikije intambwe ya 2 yigitabo gikubiyemo amabwiriza.Birasabwa gukoresha amazi ya RNase na DNase yubusa kugirango ikure hamwe nubunini bwa 100µL.

 


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