Umuntu CYP2C9 na VKORC1 Gene Polymorphism

Ibisobanuro bigufi:

Iki gikoresho kirakoreshwa muri vitro yujuje ubuziranenge bwa polymorphism ya CYP2C9 * 3 (rs1057910, 1075A> C) na VKORC1 (rs9923231, -1639G> A) muri ADN ya genomic ya ADN yuzuye y'amaraso.


Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-GE014A-Umuntu CYP2C9 na VKORC1 Gene Polymorphism Detection Kit (Fluorescence PCR)

Icyemezo

CE / TFDA

Epidemiologiya

Warfarin ni anticoagulant yo mu kanwa ikunze gukoreshwa mubikorwa byubuvuzi muri iki gihe, igamije ahanini gukumira no kuvura indwara ziterwa na tromboembolique.Nyamara, warfarin ifite idirishya rito ryo kuvura kandi iratandukanye cyane mumoko atandukanye.Imibare yerekanye ko itandukaniro ryimiti ihamye mubantu batandukanye rishobora kuba inshuro zirenga 20.Amaraso mabi aturuka ku 15.2% by'abarwayi bafata warfarin buri mwaka, muri bo 3,5% bakagira amaraso yica.Ubushakashatsi bwa Pharmacogenomic bwerekanye ko polymorphism genetique ya enzyme yintego VKORC1 na enzyme ya metabolike CYP2C9 ya warfarin ari ikintu cyingenzi kigira ingaruka ku itandukaniro ryimiti ya warfarin.Warfarin ni ikintu cyihariye cya vitamine K epoxide reductase (VKORC1), bityo ikabuza guhuza ibintu byangiza umubiri birimo vitamine K kandi igatanga anticoagulation.Umubare munini wubushakashatsi wagaragaje ko gene polymorphism ya porotokoro ya VKORC1 aricyo kintu cyingenzi kigira ingaruka kumoko no gutandukana kwabantu kugipimo gikenewe cya warfarin.Warfarin ikoreshwa na CYP2C9, kandi ihinduka ryayo ritinda cyane metabolism ya warfarin.Abantu bakoresha warfarin bafite ibyago byinshi (inshuro ebyiri kugeza kuri eshatu hejuru) yo kuva amaraso mugihe cyambere cyo kuyakoresha.

Umuyoboro

FAM VKORC1 (-1639G> A)
CY5 CYP2C9 * 3
VIC / HEX IC

Ibipimo bya tekiniki

Ububiko Amazi: ≤-18 ℃
Ubuzima bwa Shelf Amezi 12
Ubwoko bw'icyitegererezo Amaraso mashya ya EDTA anticoagulated
CV ≤5.0%
LoD 1.0ng / μL
Umwihariko Nta re-reactivité hamwe nizindi nzego zikurikiranye cyane za genomuntu (gene ya muntu CYP2C19, gene ya muntu RPN2);mutation ya CYP2C9 * 13 na VKORC1 (3730G> A) hanze yurwego rwo kumenya iki gikoresho
Ibikoresho bikoreshwa Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR

Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR

QuantStudio®5 Sisitemu-Igihe nyacyo PCR

SLAN-96P Sisitemu nyayo-PCR

UmucyoCycler®480 Sisitemu nyayo-PCR

LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR

MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe

BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR

BioRad CFX Opus 96 Sisitemu Yigihe-PCR

Urujya n'uruza rw'akazi

Gusabwa gukuramo reagent: Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acide (HWTS- 3006).


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