Uburozi bwa Clostridium difficile A/B gene (C.diff)
Izina ry'igicuruzwa
HWTS-OT031A Kit yo Gupima Aside Nucleic kuri Clostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Icyemezo
CE
Ubumenyi bw'indwara
Clostridium difficile (CD), indwara iterwa na garama-positive anaerobic sporogenic Clostridium difficile, ni imwe mu ndwara zikomeye zitera indwara zo mu mara zo mu gace k'imbere. Mu buvuzi, hafi 15% ~ 25% by'impiswi ziterwa na mikorobe, 50% ~ 75% by'indwara ziterwa na mikorobe na 95% ~ 100% by'indwara ziterwa na Clostridium difficile (CDI). Clostridium difficile ni indwara iterwa n'ibintu bitandukanye, harimo ubwoko bw'indwara ziterwa na mikorobe n'ubwoko butari ubwo mu mara.
Umuyoboro
| FAM | tcdAgene |
| ROX | tcdBgene |
| VIC/HEX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | intebe |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Ubwihariye | Koresha iyi mashini kugira ngo umenye izindi ndwara zo mu mara nka Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic types, Adenovirus, rotavirus, norovirus, grippe A virus, grippe B virus na genomic ADN y'umuntu, ibisubizo byose ni bibi. |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.) LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo (FQD-96A，HangzhouIkoranabuhanga rya Bioer) MA-6000 Real-Time Quantitative Thermocycler (Suzhou Molarray Co., Ltd.) Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Amahitamo ya 1.
Shyiramo 180μL ya lysozyme buffer kuri precipitate (gabanya lysozyme kuri 20mg/mL ukoresheje lysozyme diluent), pipette kugirango uvange neza, hanyuma ubishyire kuri 37°C mu gihe kirenze iminota 30. Fata 1.5ml ya RNase/DNase-free centrifuge tube, hanyuma wongereho180μL y'ubugenzuzi bwiza n'ubugenzuzi bubi mu buryo bukurikiranye. Ongeraho10μL y'ubugenzuzi bw'imbere ku gipimo kigomba gupimwa, ubugenzuzi bwiza, n'ubugenzuzi bubi mu buryo bukurikiranye, kandi ukoreshe Nucleic Acid Extraction cyangwa Purification Reagent (YDP302) ya Tiangen Biotech (Beijing) Co., Ltd. kugira ngo ukurikirane gukuramo ADN, kandi ukurikize neza amabwiriza yo kuyikoresha ku ntambwe runaka. Koresha DNase/RNase free H2O kuri elution, kandi ingano ya elution isabwa ni 100μL.
Amahitamo ya 2.
Fata 1.5mL y'umuyoboro wa RNase/DNase udafite centrifuge, hanyuma wongereho 200μL y'umuyoboro mwiza wo kugenzura hamwe n'umuyoboro mubi wo kugenzura uko bikurikirana.10μL y'ubugenzuzi bw'imbere ku gipimo kigomba gupimwa, ubugenzuzi bwiza, n'ubugenzuzi bubi mu buryo bukurikiranye, kandi ukoreshe Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha, kandi ingano ya elution isabwa ni 80μL.
