Clostridium diffisile toxin a / b gene (c.iff)
Izina ry'ibicuruzwa
Hwts-ot031A nucleic acide imenyekanisha rit kuri clostridium difficile toxin a / b gene (c.iff) (fluorescence pcr)
Icyemezo
CE
Epidemiology
Clostridium Difficiile (CD), gram-nziza ya anaerobic parstridium difficium difficiile, nimwe mumiterere nyamukuru itera indwara zuzuye zanduza. Caltinal, hafi 15% ~ 25% by'impiswi itemewe, 50% ~ 75% by'ibice bifitanye isano na 95% ~ 100% by'ibice bya Pseudomemckanous biterwa na Clostridium divafi (CDI). Clostridium Difosile ni pathogen iteganijwe, harimo imihangayiko ya toxigenic hamwe nuburemere butari toxigenic.
Umuyoboro
| Umuryango | TCDAgene |
| Rox | TCDBgene |
| Vic / Hex | Kugenzura imbere |
Tekinike
| Ububiko | ≤-18 ℃ |
| Imibereho-Ubuzima | Amezi 12 |
| Ubwoko bw'ikigereranyo | intebe |
| Tt | ≤38 |
| CV | ≤5.0% |
| Lod | 200cfu / ml |
| Umwihariko | Koresha Ibi bikoresho kugirango umenye indi page yimbaho nka EscheriChia Coli, StapyLo ADN ya Genomic, ibisubizo byose nibibi. |
| Ibikoresho bisabwa | Gushyira mu bikorwa biosystems 7500 igihe cya sisitemu ya PCR Gushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Umutobe®5-igihe nyacyo PCR sisitemu Slan-96p Sisitemu NYAKURI PCR (Tekinoroji yubuvuzi Cology Co, Ltd.) Umucyo®480 nyamara-sisitemu ya PCR Linegene 9600 wongeyeho sisitemu yigihe cya PCR (FQD-96A,HangzhouIkoranabuhanga rya Bioer) Ma-6000 nyayo-nyayo Umwanya wa Cyrmal Cycler (Suzhou Morlary Co, Ltd.) Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Ihitamo rya 1.
Ongeraho 180μl ya lysozmere buffer kugeza ku mvubo hanyuma wongere180μl yo kugenzura neza no kugenzura nabi murwego rukurikira. Ongeraho10μl yo kugenzura imbere icyitegererezo kugirango upimeke, kugenzura neza, no kugenzura nabi muburyo bwa nucleic (YDP302) na Tiangen) na Tiangen Nyamuneka ukurikize neza amabwiriza yo gukoresha intambwe runaka. Koresha DNASE / RNASE Ubuntu H.2O Kubitsa, hamwe nubunini bwabisabwa ni 100μl.
IHitamo 2.
Fata 1.5ml ya RNASE / DNASE CENTRIFUGE CENTRIFUGE, hanyuma wongere 200μl yo kugenzura neza no kugenzura nabi murwego rubi. Ongeraho10μl yo kugenzura imbere icyitegererezo kugirango upimeke, kugenzura neza, no kugenzura nabi ADNA / CNA (Hnat (HWTS-3004-42, hwts-3004- 96) na Macro & Micro-Ikizamini Automatic Automacs acide ya acide (HWTS-3006). Gukuramo bigomba gukorwa muburyo bukomeye amabwiriza yo gukoresha, kandi umubumbe wabisabwa ni 80μl.
