Clostridium diffile toxine A / B gene （C.diff）
Izina ryibicuruzwa
HWTS-OT031A Nucleic Acide Detection Kit ya Clostridium difficile toxine A / B gene (C.diff) (Fluorescence PCR)
Icyemezo
CE
Epidemiologiya
Clostridium difficile (CD), garama nziza ya anaerobic sporogenic Clostridium difficile, ni imwe mu mpamvu zitera indwara zanduza amara nosocomial. Mubuvuzi, hafi 15% ~ 25% byimpiswi ziterwa na mikorobe, 50% ~ 75% ya kolite ifitanye isano na mikorobe na 95% ~ 100% ya enterite ya pseudomembranous iterwa na Clostridium difficile infection (CDI). Clostridium difficile ni indwara itera indwara, harimo ubwoko bwa toxigenic hamwe nubwoko butari uburozi.
Umuyoboro
| FAM | tcdAgene |
| ROX | tcdBgene |
| VIC / HEX | Igenzura ryimbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | intebe |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU / mL |
| Umwihariko | koresha iki gikoresho kugirango umenye izindi ndwara zifata amara nka Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Itsinda B Streptococcus, Clostridium difficile idafite virusi, virusi ya Adenovirus, rotavirus, norovirus, virusi ya grippe A, virusi ya grippe A, virusi ya grippe A, virusi ya grippe A, virusi ya grippe A, virusi ya grippe A, virusi ya grippe A, virusi ya grippe A, virusi ya grippe A, virusi ya grippe, virusi ya grippe A, virusi ya grippe, virusi ya grippe A, virusi ya grippe, virusi ya grippe A, virusi ya grippe, virusi ya grippe A, virusi ya grippe, virusi ya grippe, virusi ya grippe A, Adenovirus, rotavirus, norovirus. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR (Hongshi Medical Technology Co., Ltd.) Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR ()FQD-96A，HangzhouIkoranabuhanga rya Bioer) MA-6000 Igihe Cyuzuye Cyumubare Wumukino Wamagare (Suzhou Molarray Co., Ltd.) BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Ihitamo 1.
Ongeramo 180μL ya buffer ya lysozyme kumvura (shyira lysozyme kuri 20mg / mL hamwe na lysozyme diluent), pipette kuvanga neza, hanyuma utunganyirize kuri 37 ° C muminota irenga 30. Fata 1.5mL ya RNase / DNase idafite centrifuge, hanyuma wongereho180μL yo kugenzura neza no kugenzura nabi muburyo bukurikiranye. Ongeraho10μL yo kugenzura imbere imbere yicyitegererezo igomba kugeragezwa, kugenzura neza, no kugenzura nabi muburyo bukurikiranye, kandi ukoreshe Nucleic Acide Extraction or Purification Reagent (YDP302) na Tiangen Biotech (Beijing) Co., Ltd. kugirango ikuremo ADN ikurikira, kandi nyamuneka ukurikize amabwiriza yo gukoresha intambwe zihariye. Koresha DNase / RNase kubuntu H.2O yo gukuraho, kandi ingano yo gusabwa ni 100μL.
Icya 2.
Fata 1.5mL ya RNase / DNase idafite umuyoboro wa centrifuge, hanyuma wongere 200μL yo kugenzura neza no kugenzura nabi muburyo bukurikiranye. Ongeraho10μL yo kugenzura imbere muri sample kugirango igeragezwe, igenzure neza, hamwe no kugenzura nabi muburyo bukurikiranye, kandi ukoreshe Macro & Micro-Test Virus ADN / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006). Gukuramo bigomba gukorwa bikurikije amabwiriza yo gukoresha, kandi ingano yo gusabwa ni 80μL.
