Mycobacterium Igituntu INH Kurwanya

Ibisobanuro bigufi:

Iki gikoresho gikoreshwa mukumenya neza ihinduka ryimiterere ya gene ya 315th amino acide ya gene ya katG (K315G> C) hamwe na mutation ya gene yakarere ka porotokoro ya InhA (- 15 C> T).

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

izina RY'IGICURUZWA

HWTS-RT002A-Mycobacterium Igituntu Isoniazid Kurwanya Kurwanya Ibikoresho (Fluorescence PCR)

Icyemezo

Myanmar FDA

Epidemiologiya

Isoniazid, umuti w'ingenzi urwanya igituntu watangijwe mu 1952, ni umwe mu miti ifatika yo kuvura igituntu gikora hamwe n'umuti umwe w’igituntu cyihishe.

KatG ni gene nyamukuru ikubiyemo catalase-peroxidase na mutG gene ihinduka irashobora guteza imbere synthesis yurukuta rwa selile ya mycolike, bigatuma bagiteri zirwanya isoniazid.Imvugo ya KatG ihujwe nabi nimpinduka muri INH-MIC, no kugabanuka inshuro 2 mumagambo ya katG bivamo kwiyongera gato gato inshuro 2 muri MIC.Indi mpamvu itera kurwanya isoniazid muri mycobacterium igituntu kibaho mugihe kwinjiza, gusiba cyangwa guhinduka bibaye muri InhA gene locus ya mycobacterium igituntu.

Umuyoboro

ROX	inhA (-15C> T) urubuga ·
CY5	urubuga katG (315G> C)
VIC (HEX)	IS6110

Ibipimo bya tekiniki

Ububiko	≤-18 ℃ Mu mwijima
Ubuzima bwa Shelf	Amezi 12
Ubwoko bw'icyitegererezo	Amacandwe
CV	≤5.0%
LoD	1 × 10³bagiteri / mL
Umwihariko	Nta reaction ya cross-reaction hamwe na mutation yibibanza bine birwanya ibiyobyabwenge (511, 516, 526 na 531) bya gene ya rpoB hanze yurwego rwo gutahura ibikoresho. Ibikoresho bikoreshwa: Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR SLAN-96P Sisitemu nyayo-PCR UmucyoCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR