Umuntu Craf Gene V600E
Izina ry'ibicuruzwa
Hwts-tm007-umuntu Braf gene station kit (fluorescence pcr)
Icyemezo
CE / TFDA
Epidemiology
Ubwoko burenga 30 bwa Braf yabonetse, muri byo hafi 90% biherereye muri Exon 15, aho umurongo wa V600e ufatwa nk'umubanga, ni ukuvuga, thymine (t) ku mwanya wa 1799 muri Exon 15 urimo Adenine (a), bikaviramo gusimbuza vine (v) kumwanya wa 600 na acide ya glutamic (e) mubicuruzwa bya poroteyine. Ihinduka rya Craf rikunze kuboneka mu bibyimba bibi nka Melanoma, kanseri y'amaraso, kanseri ya tiroyide, na kanseri y'ibihaha. Gusobanukirwa gutahura gene ya Braf byabaye ngombwa kwerekana egfr-tki na braf gene ya Braf Gen Rutation-imiti igamije imiti yibiyobyabwenge ishobora kungukirwa.
Umuyoboro
| Umuryango | V600e mutation, kugenzura imbere |
Tekinike
| Ububiko | ≤-18 ℃ |
| Imibereho-Ubuzima | Amezi 9 |
| Ubwoko bw'ikigereranyo | Paraffin-Yashyizwemo Paspologiya |
| CV | <5.0% |
| Ct | ≤38 |
| Lod | Koresha ibikoresho kugirango umenye kugenzura neza. a) munsi ya 3ng / μl Ubwoko bwishyamba, Igipimo cya 1% cya Mutution kirashobora kugaragara muri reaction buffer isanzwe; b) munsi yigiciro cya 1%, mutation ya 1 × 103Amakopi / ML Muburyo-bwishyamba Amateka ya 1 × 105Amakopi / ML irashobora kugaragara muburyo bukabije. c) Buffer ya IC Buffer irashobora kumenya uburyo buke bwo kumenya ubuziranenge bwiza sw3 yo kugenzura imbere. |
| Ibikoresho bisabwa: | Gushyira mu bikorwa biosystems 7500 igihe cya sisitemu ya PCRGukoresha Biosystems 7300 igihe cya PCR Sisitemu, Quanttudio® 5 Igihe-nyacyo PCR Umucyo®480 Igihe Cyigihe cya PCR Biorad CFX96 Igihe cya PCR |
Akazi
Basabwe gusubiramo Reagents: Qiaap DNAP FFPE Tissue Kit (56404), Tissue ya Ea Passue yanduye ADNAPOD (DP330) Yakozwe na Tiangen biotech (Beijing) Co., Ltd.
