Impinduka mu mikorere ya Gene V600E ya BRAF y'umuntu
Izina ry'igicuruzwa
HWTS-TM007-Human BRAF Gene V600E Mutation Detection Kit (Fluorescence PCR)
Icyemezo
CE/TFDA
Ubumenyi bw'indwara
Habonetse ubwoko burenga 30 bw'impinduka za BRAF, muri zo hafi 90% biherereye muri exon 15, aho impinduka za V600E zifatwa nk'impinduka zikunze kugaragara cyane, ni ukuvuga thymine (T) iri ku mwanya wa 1799 muri exon 15 ihinduka adenine (A), bigatuma valine (V) iri ku mwanya wa 600 isimburwa na aside glutamic (E) mu gicuruzwa cya poroteyine. Impinduka za BRAF zikunze kuboneka mu tumori twa kanseri nka melanoma, kanseri y'inkondo y'umura, kanseri ya tiroyide, na kanseri y'ibihaha. Gusobanukirwa impinduka za gene ya BRAF byabaye ngombwa gusuzuma imiti igamije guhindura EGFR-TKIs na BRAF mu miti igamije kuvurwa mu buryo bw'ubuvuzi ku barwayi bashobora kungukirwa.
Umuyoboro
| FAM | Impinduka za V600E, igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 9 |
| Ubwoko bw'ingero | ingero z'uturemangingo tw'indwara twashyizwemo parafini |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | Koresha ibikoresho kugira ngo umenye ubuziranenge bwa LoD bujyanye nabyo. a) munsi ya 3ng/μL wild-type background, 1% mutation rate irashobora kugaragara muri reaction buffer neza; b) munsi ya 1% mutation rate, 1 × 103Kopi/mL mu ishusho y'inyuma ya 1×105Kopi/mL zishobora kugaragara neza muri reaction buffer; c) IC Reaction Buffer ishobora kumenya ubuziranenge SW3 bwo hasi cyane bw’igenzura ry’imbere ry’ikigo. |
| Ibikoresho Bikoreshwa: | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500Porogaramu ya Biosystems 7300 Real-Time PCR Sisitemu, QuantStudio® 5 Sisitemu za PCR zo mu gihe nyacyo Sisitemu ya LightCycler®480 Real-Time PCR Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Ibikoresho bikoreshwa mu gukuramo ibinyabutabire: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) yakozwe na Tiangen Biotech (Beijing) Co., Ltd.
