Agakoresho ko Gupima Acide Nucleic ya Herpes Simplex yo mu bwoko bwa 1 (Fluorescence PCR)
Izina ry'igicuruzwa
Agakoresho ko Gupima Acide Nucleic ya Herpes Simplex yo mu bwoko bwa 1 (Fluorescence PCR)
Ubumenyi bw'indwara
Indwara zandurira mu mibonano mpuzabitsina (STDs) ziracyari zimwe mu ngingo zikomeye ku mutekano w’ubuzima rusange ku isi, zishobora gutera ubugumba, kubyara imburagihe, ibibyimba n’izindi ngorane zikomeye zitandukanye.[3-6]. Hariho ubwoko bwinshi bwa virusi itera indwara ya STD, harimo bagiteri, virusi, chlamydia, mycoplasma na spirochette. Ubwoko busanzwe burimo neisseria gonorrhoeae, mycoplasma genitalium, chlamydia trachomatis, virusi ya herpes simplex ubwoko bwa 1, virusi ya herpes simplex ubwoko bwa 2, mycoplasma hominis, ureaplasma urealyticum, nibindi.
Umuyoboro
| FAM | Virusi ya Herpes Simplex yo mu bwoko bwa 1 (HSV1) |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | -18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Igitambaro cyo mu mura cy’umugore,Igitambaro cyo mu muyoboro w'inkari cy'abagabo |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500Kopi/mL |
| Ubwihariye | Gerageza izindi ndwara zandurira mu mibonano mpuzabitsina, nka treponema pallidum, chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma hominis, mycoplasma genitalium, ureaplasma urealyticum, nibindi, nta reaction-cross-reaction. |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Imikorere
Reagents zikenewe ariko ntizitangwa
Ikoreshwa rya saline isanzwe, Macro na Micro-Test Release Reagent (HWTS-3005-8), Macro na Micro-Test General ADN/RNA Kit (HWTS-3017) na Macro na Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B), Nucleic Acid Extraction cyangwa Purification Reagent (YDP302) na Tiangen Biotech (Beijing) Co., Ltd.
Ibikoresho bikoreshwa bigomba ariko ntibitangwa
1.5mL y'umuyoboro wa DNase/RNase udafite centrifuge, DNase/RNase udafite Tip, ethanol yuzuye, pipette, centrifuge ya desktop, uruvange rwa desktop oscillating.
