Chlamydia Trachomatis, Neisseria Gonorrhoeae na Trichomonas vaginalis
Izina RY'IGICURUZWA
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae na Trichomonas vaginalis Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Chlamydia trachomatis (CT) ni ubwoko bwa mikorobe ya prokaryyotike yangiza cyane mu ngirabuzimafatizo za eukaryotic.Chlamydia trachomatis igabanijwemo AK serotypes ukurikije uburyo bwa serotype.Indwara ya Urogenital iterwa ahanini na trachoma biologique variant DK serotypes, kandi igitsina gabo kigaragara cyane nka urethritis, gishobora koroherwa kitavuwe, ariko inyinshi murizo ziba karande, zikomeza kwiyongera, kandi zishobora guhuzwa na epididymitis, proctitis, nibindi.
Umuyoboro
| FAM | Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Vaginite ya Trichomonal |
| VIC / HEX | Igenzura ryimbere |
Ibipimo bya tekiniki
| Ububiko | -18 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | Inkondo y'umura y'abagore abIgitsina gore swab ，Umugabo urethral swab |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Amakopi / mL |
| Umwihariko | Nta reaction-reaction hamwe nizindi ndwara zandurira mu ndwara zandurira hanze y’ibipimo byapimwe, nka Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, virusi ya Herpes simplex ubwoko bwa 1, virusi ya Herpes simplex ubwoko bwa 2, Candida albicans, nibindi. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe-PCR Sisitemu Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Ihitamo 1.
Gusabwa gukuramo reagent: Pipette 1mL yicyitegererezo igomba gupimwa kuri 1.5mL ya DNase / RNase idafite umuyoboro wa centrifuge, centrifuge saa 12000rpm kuminota 3, guta ndengakamere no gukomeza kugwa.Ongeramo 200µL ya saline isanzwe kumvura kugirango ihagarare.Macro & Micro-Ikizamini rusange ADN / RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha.Icyitegererezo cyakuweho ni 200µL, naho icyifuzo cyo gukuraho ni 80µL.
Icya 2.
Gusabwa gukuramo reagent: Gukuramo Acide Nucleic Acide cyangwa ibikoresho byoza (YDP302).Gukuramo bigomba gukorwa byimazeyo ukurikije amabwiriza yo gukoresha.Ingano isabwa yo gukuraho ni 80µL.
