Enterovirus kwisi yose
Izina ry'ibicuruzwa
Hwts-ev001- Enterovirus Yose yose Nucleic Gutahura Acide Gutahura (Fluorescence PCR)
Epidemiology
Indwara y'umunwa y'intoki ni indwara yanduza iterwa na Enterovirsus (EV). Kugeza ubu, ubwoko 108 bw'ubwoko bwa Enterovirpes bwabonetse, bugabanyijemo amatsinda ane: A, B, B, B. Muri bo, Enterovirus Ev71 na Coxa16 ni pathogens nyamukuru. Indwara ibaho mu bana bari munsi yimyaka 5, kandi irashobora gutera ibya herpes kumaboko, ibirenge, umunwa, no mubindi bice. Umubare muto w'abana uzamura ingorane nka Myocardite, Pulmone Edema, na AsEptic MenoistFlitilis.
Umuyoboro
| Umuryango | Ev rna |
| Rox | Kugenzura imbere |
Tekinike
| Ububiko | ≤-18 ℃ |
| Imibereho-Ubuzima | Amezi 12 |
| Ubwoko bw'ikigereranyo | Sropheryngeal Swab,Herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| Lod | 500copies / ml |
| Ibikoresho bisabwa | Gushyira mu bikorwa biosystems 7500/7500 byihuse sisitemu ya PCR, Umutobe®5-igihe nyacyo PCR sisitemu Scan-96p sisitemu nyayo pcr sisitemu Umucyo®480 nyamara-sisitemu ya PCR Linegene 9600 wongeyeho sisitemu yigihe cya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Ihitamo rya 1.
Tekereza gukuramo ibikoresho: Macro-Ikizamini Rusange ADN / RNA Kit (HWTS-3017-50, HWTS-3017-96, HANDT-MACRO & Micro-Ikizamini Automatic Automatic Autom ( Hwts-3006B, HWTS-3006C), igomba gushyirwaho neza ukurikije amabwiriza. Umuyoboro w'icyitegererezo ni 200 μl, umubare wasabwe Umubumbe wa 80μl.
IHitamo 2.
Ibisabwa byogutwara: Macro & Micro-Ikizamini Icyitegererezo cya Regent (HWTS-3005-8), bigomba gushyirwaho neza ukurikije amabwiriza.
Ihitamo3.
Ibisabwa byogutwara: Qiaip viral rna mini kit (52904) cyangwa acide ya nucleic cyangwa igikona (ydp315-r), igomba gukurwa mubuyobozi bukomeye. Umuyoboro w'icyitegererezo ni 140 μl, Umubumbe w'ikirenga usabwa ni 60μl.
