Ubwoko umunani bwa virusi y'ubuhumekero
Izina ryibicuruzwa
HWTS-RT184-Ubwoko umunani bwa virusi yubuhumekero Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Indwara zifata imyanya y'ubuhumekero ni ubwoko bukunze kwibasirwa n'indwara z'abantu, zishobora kugaragara mu gitsina icyo ari cyo cyose, imyaka n'akarere, kandi ni imwe mu mpamvu zikomeye zitera indwara n'urupfu ku isi[1]. Indwara zandurira mu myanya y'ubuhumekero zirimo virusi ya grippe A (IFV A), virusi ya grippe B (IFV B), virusi ya syncytial respiratory, adenovirus, metapneumovirus, rhinovirus, virusi ya parainfluenza (I / II / III) na mycoplasma pneumoniae, n'ibindi.[2,3]. Ibimenyetso bya clinique nibimenyetso biterwa no kwandura inzira zubuhumekero birasa cyane, ariko kwandura indwara ziterwa na virusi zitandukanye bifite uburyo butandukanye bwo kuvura, ingaruka zo gukiza hamwe nindwara.[4,5]. Kugeza ubu, uburyo nyamukuru bwo kumenya laboratoire itera indwara zandurira mu myanya y'ubuhumekero harimo: kwigunga virusi, kumenya antigen no kumenya aside nucleic. Iki gikoresho kimenyekanisha kandi kigaragaza acide nucleic acide yihariye kubantu bafite ibimenyetso nibimenyetso byindwara zubuhumekero, ifatanije nibindi bisubizo byubuvuzi na laboratoire kugirango bifashe mugupima indwara zandurira mu myanya y'ubuhumekero.
Ibipimo bya tekiniki
| Ububiko | 2-8 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | Oropharyngeal swab; Nasopharyngeal swab |
| Ct | IFV A, IFVB, RSV, Umujyanama, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | Amakopi 200 / mL |
| Umwihariko | Reaktivite y'umusaraba: Nta reaction ya cross iri hagati ya kit na virusi ya Boca, Cytomegalovirus, virusi ya Epstein-Barr, virusi ya Herpes simplex, virusi ya varicella zoster, virusi ya Mumps, Enterovirus, virusi y'iseru, coronavirus, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydie pne. Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, ibicurane bya Haemophilus, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculose, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigat salivarius, catarrh ya Moraxella, Lactobacillus, Corynebacterium, ADN genomic muntu. Ikizamini cyo kwivanga: Hitamo mucin (60mg / mL), amaraso yabantu (50%), benephrin (2mg / mL), hydroxymethazoline (2mg / mL) 2mg / mL), chloride ya sodium hamwe na 5% ikingira (20mg / mL), beclomethasone (20mg / mL), dexamethasone (20mg / mL), fluniacetone . . |
| Ibikoresho bikoreshwa | Bikenewe kugirango wandike I detection reagent: Ikoreshwa rya Biosystems 7500 Sisitemu nyayo-PCR Sisitemu, QuantStudio®Sisitemu 5 nyayo-PCR, SLAN-96P Sisitemu Yigihe-PCR (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Yongeyeho Sisitemu Yigihe Cyiza PCR (FQD-96A, Ikoranabuhanga rya Hangzhou Bioer), MA-6000 Igihe Cyuzuye Cyumubyigano Cyamashanyarazi (Suzhou Molarray Co, Ltd), BioRad CFX96 Real-Time Bikoreshwa muburyo bwa II bwo gutahura reagent: EudemonTM AIO800 (HWTS-EQ007) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Urujya n'uruza rw'akazi
Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3017) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B)), na Macro & Micro-Test Virus ADN / RNA Kit (HWTS-3017-8) (ishobora gukoreshwa na Eudemon.TM AIO800 (HWTS-EQ007)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ingano yicyitegererezo yakuweho ni 200μL naho ingano yo gusabwa ni 150μL.
