Agakoresho ko Gupima Aside ya Nucleic mu Gitsina cya Gardnerella (Fluorescence PCR)
Izina ry'igicuruzwa
Agakoresho ko Gupima Aside ya Nucleic mu Gitsina cya Gardnerella (Fluorescence PCR)
Ubumenyi bw'indwara
Impamvu ikunze gutera indwara ya vaginitis ku bagore ni vaginosis ya bagiteri, kandi bagiteri ikomeye itera vaginosis ya bagiteri ni Gardnerella vaginalis. Gardnerella vaginalis (GV) ni indwara idatera indwara iyo iboneka mu rugero ruto. Ariko, iyo bagiteri ziganje mu gitsina zitwa Lactobacilli zigabanuka cyangwa zikavaho, bigatuma habaho ubusumbane mu gitsina, Gardnerella vaginalis iragwira cyane, bigatuma habaho vaginosis ya bagiteri. Muri icyo gihe, izindi ndwara (nka Candida, Neisseria gonorrhoeae, Chlamydia trachomatis, nibindi) zishobora kwinjira mu mubiri w'umuntu, zigatera vaginitis ivanze na cervicitis. Iyo vaginitis na cervicitis bitasuzumwe kandi bikavurwa ku gihe kandi neza, hashobora kubaho indwara zizamuka ziturutse ku mikorobe ku gice cy'imyanya myibarukiro, bigatera indwara zo mu gice cyo hejuru cy'imyanya myibarukiro nka endometrite, salpingitis, tubo-ovarian abscess (TOA), na peritonitis yo mu kibuno, bishobora gutera ingorane zikomeye nko kutabyara, gutwita hanze y'igitsina ndetse n'ingaruka mbi zo gutwita.
Ibipimo bya tekiniki
| Ububiko | -18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | ifu y'inkari y'abagabo, ifu y'inkari y'abagore, ifu y'abagore mu gitsina |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Kopi 400/mL |
| Ibikoresho Bikoreshwa | Ikoreshwa kuri reagent yo gutahura ubwoko bwa I:Porogaramu za mudasobwa zikoreshwa mu buryo bwa "Real-Time PCR Systems", QuantStudio®Sisitemu 5 za PCR zikora mu gihe nyacyo, Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.), Sisitemu zo Gupima za LineGene 9600 Plus mu gihe nyacyo (FQD-96A, ikoranabuhanga rya Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermocycler (Suzhou Molarray Co., Ltd.), Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo, Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo. Ikoreshwa ku gikoresho cyo gutahura ubwoko bwa II: EudemonTMAIO800 (HWTS-EQ007) yakozwe na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Imikorere
Reagent irakenewe ariko ntabwo itanzwe
Kit ya saline isanzwe, Macro & Micro-Test General ADN/RNA (HWTS-3019) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yakozwe na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ibikoresho bikenewe ariko ntibitangwa
Amasasu adafite RNase/DNase, uturindantoki dukoreshwa rimwe, imiyoboro idafite RNase/DNase, centrifuge, icyuma gihagarara gikoresheje magnetique, uturongo tw'imiyoboro 8 twa PCR.
