Chlamydia Trachomatis, Neisseria Gonorrhoeae na Trichomonas vaginalis
Izina ry'igicuruzwa
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae na Trichomonas vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
Ubumenyi bw'indwara
Chlamydia trachomatis (CT) ni ubwoko bw'uturemangingo duto twa prokaryotic twibasira cyane uturemangingo twa eukaryotic. Chlamydia trachomatis igabanyijemo serotypes za AK hakurikijwe uburyo bwa serotype. Indwara zo mu nzira y'ubura ziterwa ahanini na serotypes za trachoma biological variant DK, kandi abagabo bakunze kugaragara nk'indwara ya urethritis, ishobora kuvura hatabayeho kuvurwa, ariko inyinshi muri zo ziba zidakira, rimwe na rimwe zikongera ubukana, kandi zishobora guhuzwa na epididymitis, proctitis, nibindi.
Umuyoboro
| FAM | Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Trichomonal vaginitis |
| VIC/HEX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | -18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Igitambaro cyo mu mura cy’umugore,Igitambaro cyo mu gitsina cy'umugore,Igitambaro cyo mu muyoboro w'inkari cy'abagabo |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Kopi/mL |
| Ubwihariye | Nta buryo bwo guhuza indwara zandurira mu mibonano mpuzabitsina bushobora kubaho iyo umuntu adafite aho ahuriye n’izindi ndwara zandurira mu mibonano mpuzabitsina, nka Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, virusi ya Herpes simplex type 1, virusi ya Herpes simplex type 2, Candida albicans, nibindi. |
| Ibikoresho Bikoreshwa | Sisitemu ya PCR ikoreshwa mu gihe nyacyo ya 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Amahitamo ya 1.
Reagent isabwa gukurwamo: Pipette 1mL y'icyitegererezo igomba gupimwa kuri 1.5mL y'umuyoboro wa centrifuge wa DNase/RNase-free, centrifuge kuri 12000rpm mu gihe cy'iminota 3, jugunya supernatant hanyuma ugumane precipitate. Ongeraho 200µL y'umunyu usanzwe kuri precipitate kugira ngo wongere uhume. Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Gukurwamo bigomba gukorwa hakurikijwe amabwiriza yo gukoreshwa. Ingano y'icyitegererezo yakuwemo ni 200µL, naho ingano y'icyitegererezo yatanzwe ni 80µL.
Amahitamo ya 2.
Uburyo bwo gukuramo aside ya Nucleic busabwa: Kit yo gukuramo aside ya Nucleic cyangwa Purification (YDP302). Gukuramo aside bigomba gukorwa neza hakurikijwe amabwiriza yo kuyikoresha. Ingano ya elution isabwa ni 80µL.
