Ubwoko 29 bw'udukoko dutera indwara zo mu myanya y'ubuhumekero aside nucleique ivanze
Izina ry'igicuruzwa
Ubwoko bwa HWTS-RT160 -29 bw'udukoko duhumeka. Akamashini ko gupima aside nucleic kavanze
Ubumenyi bw'indwara
Indwara zo mu myanya y'ubuhumekero ni indwara ikunze kugaragara cyane mu bantu, ishobora kugaragara mu gitsina icyo ari cyo cyose, imyaka n'akarere. Ni imwe mu mpamvu zikomeye zitera indwara n'impfu mu baturage ku isi [1]. Indwara zisanzwe zitera indwara zo mu myanya y'ubuhumekero zirimo coronavirus nshya, virusi ya Grippe A, virusi ya Grippe B, virusi ya Syncytial yo mu myanya y'ubuhumekero, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus type I/II/III, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, na Streptococcus pneumoniae, nibindi [2,3]. Ibimenyetso n'ibimenyetso biterwa n'indwara zo mu myanya y'ubuhumekero birasa, ariko uburyo bwo kuvura, ubushobozi n'uburyo indwara ziterwa n'indwara zitandukanye zigenda ziratandukanye [4,5]. Muri iki gihe, uburyo bw'ingenzi bukoreshwa muri laboratwari mu gutahura indwara zo mu myanya y'ubuhumekero zavuzwe haruguru burimo: kwitandukanya na virusi, kumenya antigen no kumenya aside nucleic, nibindi. Iyi kit ipima kandi ikamenya aside nucleic ya virusi ku bantu bafite ibimenyetso n'ibimenyetso by'indwara zo mu myanya y'ubuhumekero, hamwe no kumenya virusi za grippe na coronavirus, kandi ihuzwa n'ibindi bisubizo bya laboratwari kugira ngo ifashe mu gusuzuma indwara zo mu myanya y'ubuhumekero. Ibisubizo bibi ntibikuraho ubwandu bwa virusi zo mu myanya y'ubuhumekero kandi ntibigomba gukoreshwa nk'ishingiro ryonyine ryo gusuzuma, kuvura, cyangwa gufata ibyemezo byo kuvura. Ibisubizo byiza ntibishobora gukuraho ubwandu bwa bagiteri cyangwa ubwandu buvanze n'izindi virusi bitari mu bimenyetso by'ibizamini. Abakora igerageza bagomba kuba barahawe amahugurwa y'umwuga mu kongera imiterere y'uturemangingo cyangwa gupima ibinyabuzima, kandi bafite impamyabumenyi z'igerageza zijyanye n'imikorere. Laboratwari igomba kugira ibikoresho byo kwirinda uburibwe n'uburyo bwo kwirinda.
Ibipimo bya tekiniki
| Ububiko | -18℃ |
| Igihe cyo kumara | Amezi 9 |
| Ubwoko bw'ingero | Agapfukamunwa ko mu muhogo |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Kopi 200/mL |
| Ubwihariye | Ibisubizo by'ibizamini bya "cross-reactivity" byagaragaje ko nta "cross-reaction" yagaragaye hagati y'iyi kit na Cytomegalovirus, virusi ya Herpes simplex type 1, virusi ya Varicella-zoster, virusi ya Epstein-Barr, Pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, ubwoko bwa Mycobacterium tuberculosis bwagabanutse, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii, na aside nucleic nucleic y'umuntu. |
| Ibikoresho Bikoreshwa | Porogaramu za mudasobwa zikoreshwa mu buryo bwa "Real-Time PCR Systems",Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500, QuantStudio®Sisitemu 5 za PCR zikora mu gihe nyacyo, Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.), LightCycler®Sisitemu ya PCR ya 480 ikora mu gihe nyacyo, Sisitemu zo Gupima za LineGene 9600 Plus mu gihe nyacyo (FQD-96A, ikoranabuhanga rya Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermocycler (Suzhou Molarray Co., Ltd.), Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo, Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo. |
Urugendo rw'akazi
Kit ya Macro & Micro-Test Viral DNA/RNA (HWTS-3017) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), na Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (ishobora gukoreshwa hamwe na EudemonTM AIO800 (HWTS-EQ007)) yakozwe na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ingano y'icyitegererezo cyakuwemo ni 200μL naho ingano y'icyitegererezo cyo kuyikuraho ni 150μL.
