Ubwoko bwa Pathogene 29 Zihumeka Acide Nucleic Acide
Izina ryibicuruzwa
HWTS-RT160 -29 Ubwoko bwa virusi itera ubuhumekero bwahujwe na Nucleic Acide Detection Kit
Epidemiologiya
Indwara z'ubuhumekero ni indwara ikunze kugaragara ku bantu, ishobora kugaragara mu gitsina icyo ari cyo cyose, imyaka ndetse n'akarere. Ni imwe mu mpamvu zitera indwara n’impfu ku baturage ku isi hose [1]. Indwara zandurira mu myanya y'ubuhumekero zirimo coronavirus nshya, virusi ya grippe A, virusi ya grippe B, virusi ya syncytial virusi, Adenovirus, metapneumovirus y'abantu, rhinovirus, virusi ya Parainfluenza I / II / III, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Mycoplasma pneumoniae umusonga, n'ibindi [2,3]. Ibimenyetso n'ibimenyetso biterwa n'indwara z'ubuhumekero birasa cyane, ariko uburyo bwo kuvura, gukora neza n'inzira zandura ziterwa na virusi zitandukanye ziratandukanye [4,5]. Kugeza ubu, uburyo bw’ibanze bukoreshwa muri laboratoire kugira ngo hamenyekane indwara ziterwa n’ubuhumekero twavuze haruguru zirimo: kwigunga kwa virusi, kumenya antigen no kumenya aside nucleique, n'ibindi. Ibisubizo bibi ntibikuraho kwandura virusi yubuhumekero kandi ntibigomba gukoreshwa nkishingiro ryonyine ryo gusuzuma, kuvura, cyangwa ibindi byemezo byubuyobozi. Igisubizo cyiza ntigishobora kwirinda kwandura bagiteri cyangwa kwandura kuvanze nizindi virusi ziri hanze yerekana ibipimo. Abakora ubushakashatsi bagombye kuba barahawe amahugurwa yumwuga muguhindura gene cyangwa kumenya ibinyabuzima bya molekuline, kandi bafite impamyabumenyi yubushakashatsi. Laboratoire igomba kuba ifite uburyo bwiza bwo gukumira ibinyabuzima no kwirinda.
Ibipimo bya tekiniki
| Ububiko | -18 ℃ |
| Ubuzima bwa Shelf | Amezi 9 |
| Ubwoko bw'icyitegererezo | Umuhogo |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200Copi / μL |
| Umwihariko | Ibisubizo by'ibizamini bya cross-reactivite byerekanye ko nta reaction yakozwe hagati yiki gikoresho na Cytomegalovirus, virusi ya Herpes simplex yo mu bwoko bwa 1, virusi ya Varicella-zoster, virusi ya Epstein-Barr, Pertussis, Corynebacterium, Escherichia coli, Haemophilus grippe, Lactobacillus, Legionella pneumophila. Igituntu cya Mycobacterium, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophilonas striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Klebsiella pneumoniae acide nucleic. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500-Igihe-PCR Sisitemu, Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-PCR Sisitemu, QuantStudio®5 Sisitemu nyayo-PCR Sisitemu, SLAN-96P Sisitemu nyayo-PCR (Hongshi Medical Technology Co., Ltd.), Umucyo®480 Sisitemu nyayo-PCR, LineGene 9600 Yongeyeho Sisitemu Yukuri-PCR Yerekana (FQD-96A, Ikoranabuhanga rya Hangzhou Bioer), MA-6000 Igihe Cyuzuye Cyumubyigano Wumukino (Suzhou Molarray Co., Ltd.), BioRad CFX96 Sisitemu nyayo-PCR Sisitemu, BioRad CFX Opus 96 Sisitemu nyayo-PCR. |
Urujya n'uruza rw'akazi
Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3017) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B)), na Macro & Micro-Test Virus ADN / RNA Kit (HWTS-3017-8) (ishobora gukoreshwa na Eudemon.TM AIO800 (HWTS-EQ007)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ingano yicyitegererezo yakuweho ni 200μL naho ingano yo gusabwa ni 150μL.
