14 Ubwoko bwa Papillomavirus Yumuntu Wibyago Byinshi (18/18/52 Kwandika)
Izina RY'IGICURUZWA
HWTS-CC019A-14 Ubwoko bwa Papillomavirus Yumuntu Wibyago Byinshi (16/18/52 Kwandika) Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Ubushakashatsi bwerekanye ko HPV yanduye kandi yanduye ni imwe mu mpamvu zitera kanseri y'inkondo y'umura.Kugeza ubu, imiti izwi neza iracyabura kanseri y'inkondo y'umura iterwa na HPV, bityo kuvumbura hakiri kare no kwirinda kwandura inkondo y'umura iterwa na HPV ni urufunguzo rwo kwirinda kanseri y'inkondo y'umura.Ni ingirakamaro cyane gushyiraho ikizamini cyoroshye cyo gusuzuma indwara ya etiologiya yo kwisuzumisha no kuvura kanseri y'inkondo y'umura.
Umuyoboro
| Umuyoboro | Andika |
| FAM | HPV 18 |
| VIC / HEX | HPV 16 |
| ROX | HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 |
| CY5 | HPV 52 |
| Quasar 705/CY5.5 | Igenzura ryimbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | Inkari Sw Inkondo y'umura ， Igituba |
| Ct | ≤28 |
| LoD | Amakopi 300 / mL |
| Umwihariko | Nta reaction-reaction hamwe nizindi ngero zubuhumekero nka Grippe A, Grippe B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, virusi yubuhumekero, Parainfluenza 1, 2, 3, virusi ya Coxsackie, Echo virusi, Metapneumovir B1 / B2, virusi yubuhumekero A / B, Coronavirus 229E / NL63 / HKU1 / OC43, Rhinovirus A / B / C, virusi ya Boca 1/2/3/4, Chlamydia trachomatis, adenovirus, nibindi na ADN ya ADN. |
| Ibikoresho bikoreshwa | MA-6000 Igihe Cyuzuye Cyumubare Wumukino Wamagare (Suzhou Molarray Co., Ltd.) BioRad CFX96 Sisitemu Yigihe-PCR na BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
1.Icyitegererezo cy'inkari
Igisubizo: Fata1.4mL ya sample yinkari igomba gupimwa na centrifuge saa 12000rpm kuminota 5;guta ndengakamere (birasabwa kugumya 10-20μL ndengakamere kuva munsi yumuyoboro wa centrifuge), ongeramo 200μL yicyitegererezo cyo kurekura reagent, hanyuma gukuramo nyuma bigomba gukorwa ukurikije amabwiriza yo gukoresha Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).
B: Fata1.4mL y'icyitegererezo cy'inkari kugeragezwa na centrifuge saa 12,000rpm muminota 5;guta ndengakamere (birasabwa kubika 10-20μL ya supernatant kuva munsi yumuyoboro wa centrifuge), hanyuma ukongeramo 200μL yumunyu usanzwe kugirango uhagarare, nkicyitegererezo cyo gupimwa.Gukuramo gukurikiraho birashobora gukorwa hamwe na Macro & Micro-Test Virus ADN / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. hakurikijwe amabwiriza.s Kuri Koresha.Ingano isabwa yo gukuraho ni 80μL.
C: Fata1.4mL y'icyitegererezo cy'inkari kugeragezwa na centrifuge saa 12,000rpm muminota 5;guta ndengakamere (birasabwa kubika 10-20μL ya ndengakamere kuva munsi yumuyoboro wa centrifuge), hanyuma ukongeramo 200μL yumunyu usanzwe kugirango uhagarare, nkicyitegererezo cyo gupimwa.Gukuramo gukurikiraho birashobora gukorwa hamweQIAamp ADN Mini Kit (51304) na QIAGEN cyangwa Macro & Micro-Ikizamini cya virusi ADN / Inkingi ya RNA (HWTS-3020-50).Gukuramo bigomba gutunganywa ukurikije amabwiriza yo gukoresha.Ingano yo gukuramo urugero ni 200μL, kandi ibyifuzo bisabwa ni 80μL.
2. Cervical swab / vaginal swab sample
Igisubizo: Fata 1mL yicyitegererezo kugirango ugerageze muri 1.5mLof centrifuge tube,nacentrifuge saa 12000rpm kuminota 5. Discard ndengakamere (birasabwa kubika 10-20μL yindengakamere kuva munsi yumuyoboro wa centrifuge), ongeramo 100μL yicyitegererezo cyo kurekura reagent, hanyuma ukuremo ukurikije amabwiriza yo gukoresha Macro & Micro-Test Sample Release Reagent ( HWTS-3005-8).
B: Gukuramo birashobora gukorwa hamwe na Macro & Micro-Test Virus ADN / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. hakurikijwe amabwiriza yo gukoresha.Ingano yicyitegererezo yakuweho ni 200μL, naho icyifuzo cyo gukuraho ni 80μL.
C: Gukuramo birashobora gukorwa hamwe na QIAamp ADN Mini Kit (51304) na QIAGEN cyangwa Macro & Micro-Test Virus ADN / Inkingi ya RNA (HWTS-3020-50).Gukuramo bigomba gutunganywa ukurikije amabwiriza yo gukoresha.Gukuramo icyitegererezo ni 200 μL, kandi ingano yo gusabwa ni80 μL.
3 、 Inkondo y'umura/ Igituba
Mbere yo gutoranya, koresha ipamba kugirango uhanagure witonze ururenda rurenze inkondo y'umura, hanyuma ukoreshe irindi pamba ryinjiye hamwe nigisubizo cyo kubika ingirabuzimafatizo cyangwa inkondo y'umura ya selfile selile yohasi kugirango wizirike kuri mucosa yinkondo y'umura hanyuma uhindukire ku isaha 3-5 kugirango ubone inkondo y'umura.Buhoro buhoro fata ipamba cyangwa brush,nashyira mucyitegererezo hamwe na 1mL ya saline isanzwe. After kwoza neza, kanda byumye ipamba cyangwa uhanagure kurukuta rwa tube hanyuma ujugunye, komeza igituba, hanyuma ushireho izina ryicyitegererezo (cyangwa umubare) hanyuma wandike kuri sample.
