Vancomycine irwanya Enterococcus na Gene irwanya ibiyobyabwenge
Izina RY'IGICURUZWA
HWTS-OT090-Vancomycine irwanya Enterococcus hamwe nigikoresho cyo kurwanya ibiyobyabwenge (Fluorescence PCR)
Epidemiologiya
Kurwanya ibiyobyabwenge bizwi kandi nko kurwanya ibiyobyabwenge, bivuga kurwanya bagiteri kurwanya imiti ya antibacterial.Iyo imiti imaze kurwanya, imiti ya chimiotherapie yibiyobyabwenge izagabanuka cyane.Kurwanya Ibiyobyabwenge bigabanijwemo kurwanya imbere.Kurwanya imbere kugenwa na bagiteri chromosomal genes, yagiye ikurikirana ibisekuruza, kandi ntabwo bizahinduka.Kurwanya kurwanya biterwa nuko nyuma yo guhura na antibiotike, bagiteri zihindura inzira zazo za metabolike kugirango ziticwa na antibiotike.
Indwara ya vancomycine irwanya VanA na VanB iboneka mu kurwanya ibiyobyabwenge, muri byo VanA ikerekana urugero rwinshi rwo kurwanya vancomycine na teicoplanine, VanB yerekana urwego rutandukanye rwo kurwanya vancomycine, kandi ikumva teicoplanine.Vancomycine ikunze gukoreshwa mu buvuzi kugira ngo ivure indwara ziterwa na bagiteri Gram-positif, ariko kubera ko hagaragaye enterococci irwanya vancomycine (VRE), cyane cyane enterococcus faecalis na enterococcus faecium, ikaba irenga 90%, yazanye ibibazo bikomeye mu kuvura amavuriro; .Kugeza ubu, nta muti wihariye wa antibacterial wo kuvura VRE.Ikirenzeho, VRE irashobora kandi kwanduza genes zirwanya ibiyobyabwenge izindi enterococci cyangwa izindi bagiteri nziza.
Umuyoboro
FAM | Vancomycine irwanya enterococci (VRE): Enterococcus faecalis na Enterococcus faecium |
VIC / HEX | Igenzura ryimbere |
CY5 | vancomycin irwanya gene VanB |
ROX | vancomycin irwanya gene VanA |
Ibipimo bya tekiniki
Ububiko | Amazi: ≤-18 ℃ |
Ubuzima bwa Shelf | Amezi 12 |
Ubwoko bw'icyitegererezo | spumum, amaraso, inkari cyangwa koloni nziza |
CV | ≤5.0% |
Ct | ≤36 |
LoD | 103CFU / mL |
Umwihariko | Nta reaction-reaction hamwe nizindi ndwara ziterwa nubuhumekero nka klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus A. ia coli, pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, adenovirus yubuhumekero, cyangwa ingero zirimo izindi gen zirwanya ibiyobyabwenge CTX, mecA, SME, Ingero za SHV na TEM. |
Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe-PCR Sisitemu SLAN-96P Sisitemu nyayo-PCR UmucyoCycler®480 Sisitemu nyayo-PCR |
Urujya n'uruza rw'akazi
Gusabwa gukuramo reagent: Macro & Micro-Test Genomic ADN Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) na Macro & Micro-Test Automatic Nucleic Acide (HWTS-3006C, HWTS-3006B) .