Enterococcus irwanya Vancomycin na Gene irwanya imiti
Izina ry'igicuruzwa
HWTS-OT090-Enterococcus irwanya Vancomycin hamwe n'ibikoresho byo gupima jeni bidakoresha imiti (Fluorescence PCR)
Ubumenyi bw'indwara
Ubudahangarwa ku miti buzwi kandi nka ubudahangarwa ku miti, bivuga ubudahangarwa bwa bagiteri ku ngaruka z'imiti irwanya udukoko. Iyo imiti imaze kunanirwa, ingaruka za chimiotherapie z'imiti zizagabanuka cyane. Ubudahangarwa ku miti bugabanyijemo ubudahangarwa bw'umubiri n'ubudahangarwa buterwa n'uturemangingo twa chromosomes twa bagiteri, duhererekanwa kuva ku gisekuru kijya ku kindi, kandi ntabwo bizahinduka. Ubudahangarwa buterwa n'uko nyuma yo gukora imiti irwanya udukoko, bagiteri zihindura inzira zazo bwite z'imikorere kugira ngo ziticwa na antibiyotike.
Uturemangingo twa Vancomycin twihanganira VanA na VanB ni uturemangingo twakira imiti, muri two VanA igaragaza uturemangingo twinshi twa vancomycin na teicoplanin, VanB igaragaza uturemangingo twinshi twa vancomycin, kandi ikaba ifite ubushobozi bwo kwihanganira teicoplanin. Vancomycin ikunze gukoreshwa mu buvuzi mu kuvura indwara za bagiteri za Gram-positive, ariko bitewe n’uko hagaragaye enterococci idakira vancomycin (VRE), cyane cyane enterococcus faecalis na enterococcus faecium, birenga 90%, byazanye imbogamizi nshya zikomeye mu buvuzi. Kuri ubu, nta muti wihariye wa antibacterial uhari wo kuvura VRE. Ikindi kandi, VRE ishobora no kohereza uturemangingo twa antibacterial ku zindi enterococci cyangwa izindi bagiteri za Gram-positive.
Umuyoboro
| FAM | Enterococci idakira Vancomycin (VRE): Enterococcus faecalis na Enterococcus faecium |
| VIC/HEX | Igenzura ry'imbere |
| CY5 | Jene irwanya vancomycin VanB |
| ROX | Jene irwanya vancomycin VanA |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | amatembabuzi, amaraso, inkari cyangwa ibice by'umubiri byuzuye |
| CV | ≤5.0% |
| Ct | ≤36 |
| LoD | 103CFU/mL |
| Ubwihariye | Nta buryo bwo guhuza indwara ziterwa n’izindi ndwara z’ubuhumekero nka klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus influenzae, A. junii, A. haemolyticus, legionella pneumophila, escherichia coli, pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, respiratory adenovirus, cyangwa ingero zirimo izindi gene zidashobora imiti CTX, mecA, SME, Ingero za SHV na TEM. |
| Ibikoresho Bikoreshwa | Sisitemu ya PCR ikoreshwa mu gihe nyacyo ya 7500 Sisitemu za PCR za SLAN-96P mu gihe nyacyo Sisitemu ya LightCycler®480 Real-Time PCR |
Urugendo rw'akazi
Ibikoresho byo gukuramo ibinyabutabire byasabwe: Kit ya DNA ya Macro & Micro-Test Genomic (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
