Staphylococccuc
Izina ry'ibicuruzwa
Hwts-ot062 staphylococcccus aureus aureus na methicillin-staphylocococccccus aureus aureus (Mrsa / SA) Gutahura Acid Nucleic Kumenya (Fluorescence PCR)
Icyemezo
CE
Epidemiology
Staphylococccuc nimwe muri bagiteri yingenzi ya patteri ya patsomial ya nosomial. Staphylococccucclus (sa) ni uw'icaplococcus kandi ni uhagarariye bagiteri nziza-grami, ishobora kubyara amarozi atandukanye hamwe na enzymes. Bagiteri ifite ibiranga kugabana kwagutse, pathogisity ikomeye hamwe nigipimo cyo kurwanya cyane. Gene ya Nuclease Gene (NUC) ni gene yangiritse cyane ya staphylococcccuc.
Umuyoboro
| Umuryango | Methicillin-Irwanya Meca gene |
| Rox | Kugenzura imbere |
| Cy5 | staphylococccus aureus nuc gene |
Tekinike
| Ububiko | ≤-18 ℃ & Kurindwa Umucyo |
| Imibereho-Ubuzima | Amezi 12 |
| Ubwoko bw'ikigereranyo | Indwara ya Sputum, Uruhu no Kwanduza Indwara Yintangarugero, na Nasal Swab Ingero |
| Ct | ≤36 |
| CV | ≤5.0% |
| Lod | 1000 cfu / ml staphylococcccic aureus, 1000 cfu / ml metteri yumutima methicillin-irwanya. Iyo ibikoresho byatanze igihugu cyerekanwe, 1000 / ml staphylococccis aureus irashobora kumenyekana |
| Umwihariko | Ikizamini cya Cross-reactivite cyerekana ko iyi Kit idafite imirongo yambukiranya hamwe na methicicillic nka metcheylococccus, staphylococccuc, staphichia coli, kleblichia coli, kleblichia coli, Enterusla Mirabilis, Entirobacter Cloacae, Enterobacter Feacaiae, Enterococcus PNeOniae, Enterococcus, Leraxidella Catarrhalis, Neramolila Catarrhalis, Neamophilus Driverzae. |
| Ibikoresho bisabwa | Gushyira mu bikorwa biosystems 7500 igihe cya sisitemu ya PCR Umutobe®5-igihe nyacyo PCR sisitemu Scan-96p sisitemu nyayo pcr sisitemu Umucyo®480 nyamara-sisitemu ya PCR Linegene 9600 wongeyeho sisitemu yigihe cya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Ihitamo rya 1.
Macro & Micro-Ikizamini Genomic ADN / RNA Kit (HWTS-3019) na Jiacsu Macro-Tech-Ikizamini Cyiza Acide Autocle (HWTS-3006C, hwts- 3006B). Ongeramo 200μl yiminyuhunyu gasanzwe ku mpingamubiri zitunganijwe, kandi intambwe zakurikiyeho zigomba guterwa ukurikije amabwiriza, kandi umubumbe wasabwe ni 80μl.
IHitamo 2.
Macro & Micro-Ikizamini Icyitegererezo cya Reagent (HWTS-3005-8) na Jiacsu Macro-Tech-Tech-Tech-Tech-Tech-Tech Cultination ya salle isanzwe, hanyuma uvane neza. Centrifuge kuri 13,000R / min muminota 5, kura ndengalire (kubika 10-20μl ya supernatant), hanyuma ukurikize amabwiriza yo gukuramo nyuma.
Gusaba gukuramo Reagent: Gukuramo acide nucleic cyangwa kweza ibyangombwa (YDP302) na Tiangen biotech (Beijing) Co, Ltd. Gukuramo bigomba gukorwa neza ukurikije intambwe ya 2 yigitabo cyigisha. Birasabwa gukoresha amafaranga ya RNSE na DNAse kugirango yishyure hamwe nijwi rya 100μl.
