Staphylococcus Aureus na Staphylococcus Aureus irwanya Methicillin (MRSA/SA)
Izina ry'igicuruzwa
HWTS-OT062 Staphylococcus Aureus na Staphylococcus Aureus irwanya Methicillin (MRSA/SA) Kit yo Gupima Acide Nucleic (Fluorescence PCR)
Icyemezo
CE
Ubumenyi bw'indwara
Staphylococcus aureus ni imwe muri bagiteri z’ingenzi zitera indwara zandura mu gace k’umugezi. Staphylococcus aureus (SA) ni imwe muri staphylococcus kandi ihagarariye bagiteri zifite Gram-positive, zishobora gukora uburozi butandukanye n’imisemburo yibasira. Bagiteri zifite imiterere yo gukwirakwira mu buryo bwagutse, indwara zikomeye kandi zirwanya cyane. Gene ya nuclease ikoreshwa mu kuvura (nuc) ni gene ikomeza kubikwa cyane ya staphylococcus aureus.
Umuyoboro
| FAM | gene ya mecA irwanya methicillin |
| ROX | Igenzura ry'imbere |
| CY5 | gene ya staphylococcus aureus nuc |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ kandi birinzwe n'urumuri |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | ingero z'indwara z'amakoho, uruhu n'imitsi yoroshye, hamwe n'ingero z'agapfukamunwa k'izuru |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL bagiteri zirwanya methicillin. Iyo agakoresho gasanze LoD ikoreshwa mu gihugu, 1000/mL staphylococcus aureus irashobora kuboneka |
| Ubwihariye | Ikizamini cya "cross-reactivity" kigaragaza ko iyi kit nta reactivity ifitanye isano n'izindi ndwara zifata imyanya y'ubuhumekero nka staphylococcus aureus ishobora kwanduzwa na methicillin, staphylococcus negative coagulase, staphylococcus epidermidis idakira methicillin, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Amahitamo ya 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) yakozwe na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Shyiramo 200µL y'umunyu usanzwe ku muvuduko watunganyijwe, hanyuma intambwe zikurikira zigomba gukurwamo hakurikijwe amabwiriza, kandi ingano ya elution isabwa ni 80µL.
Amahitamo ya 2.
Ikizamini cya Macro & Micro-Test Med-Tech Co., Ltd. Shyira 1mL y'umunyu usanzwe ku musemburo nyuma yo gukaraba n'umunyu usanzwe, hanyuma uvange neza. Shyira centrifuge ku gipimo cya 13.000r/min mu gihe cy'iminota 5, ukuremo umunyu usanzwe (bika 10-20µL y'umunyu usanzwe), hanyuma ukurikize amabwiriza yo gukuramo nyuma.
Reagent isabwa gukurwamo: Reagent yo gukuramo aside ya Nucleic cyangwa yo gusukura (YDP302) ya Tiangen Biotech (Beijing) Co., Ltd. Gukuramo bigomba gukorwa neza hakurikijwe intambwe ya 2 y'igitabo cy'amabwiriza. Birasabwa gukoresha amazi adafite RNase na DNase kugira ngo habeho ingano ya 100µL.
