Staphylococcus Aureus na Methicillin-Irwanya Staphylococcus Aureus (MRSA / SA)
Izina RY'IGICURUZWA
HWTS-OT062 Staphylococcus Aureus na Methicillin-Resistant Staphylococcus Aureus (MRSA / SA) Nucleic Acide Detection Kit (Fluorescence PCR)
Icyemezo
CE
Epidemiologiya
Staphylococcus aureus ni imwe muri bagiteri zikomeye zitera indwara ya nosocomial.Staphylococcus aureus (SA) ni iya staphylococcus kandi ihagarariye bagiteri nziza ya Gram-positif, ishobora kubyara uburozi butandukanye hamwe na enzymes zitera.Indwara ya bagiteri ifite ibiranga gukwirakwiza kwinshi, gutera indwara zikomeye hamwe n’igipimo kinini cyo guhangana.Thermostable nuclease gene (nuc) ni gene yabitswe cyane ya staphylococcus aureus.
Umuyoboro
| FAM | methicilline-irwanya mecA gene |
| ROX | Igenzura ryimbere |
| CY5 | staphylococcus aureus nuc gene |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ & ikingiwe urumuri |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | spumum, uruhu hamwe nuduce twanduye twanduye, hamwe nizuru rya swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU / mL staphylococcus aureus, 1000 CFU / mL ya bacteri irwanya methicilline.Iyo igikoresho kimenye LoD yigihugu, 1000 / mL staphylococcus aureus irashobora kuboneka |
| Umwihariko | Ikizamini cya cross-reactivite cyerekana ko iki gikoresho kidafite reaction yizindi ndwara ziterwa nubuhumekero nka methicillin-yunvikana na staphylococcus aureus, coagulase-negative staphylococcus, methicillin-anti-staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, kle mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilose, moraxella catarrhalis, neisseria meningitidis, grippe haemophilus. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR QuantStudio®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Ihitamo 1.
Macro & Micro-Test Genomic ADN / RNA Kit (HWTS-3019) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. irashobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS- 3006B).Ongeramo 200µL ya saline isanzwe kumvura yatunganijwe, hanyuma intambwe ikurikiraho igomba gukururwa ukurikije amabwiriza, kandi ingano isabwa ni 80µL.
Icya 2.
Urugero rwa Macro & Micro-Ikigereranyo Cyisohoka Reagent (HWTS-3005-8) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ongeramo 1mL yumunyu usanzwe mumvura nyuma yo koza hamwe na saline isanzwe, hanyuma uvange neza.Centrifuge kuri 13,000r / min muminota 5, ikureho ndengakamere (kubika 10-20µL ya supernatant), hanyuma ukurikize amabwiriza yo gukuramo nyuma.
Gusabwa gukuramo reagent: Gukuramo Acide Nucleic Acide cyangwa Isukura Reagent (YDP302) na Tiangen Biotech (Beijing) Co, Ltd.Gukuramo bigomba gukorwa cyane ukurikije intambwe ya 2 yigitabo gikubiyemo amabwiriza.Birasabwa gukoresha amazi ya RNase na DNase yubusa kugirango ikure hamwe nubunini bwa 100µL.
