Ubwoko bwa virusi ya polio Ⅰ
Izina ry'igicuruzwa
HWTS-EV006- Agakoresho ko Gupima Aside Nucleic mu bwoko bwa Poliovirusi Ⅰ (Fluorescence PCR)
Ubumenyi bw'indwara
Virusi ya Polio ni virusi itera poliomyelitis, indwara yandura ikwirakwira cyane. Iyi virusi ikunze kwinjira mu mitsi yo hagati, ikangiza uturemangingo tw’imitsi mu ihembe ry’imbere ry’umugongo, kandi igatera ubumuga bw’ingingo, ibi bikaba bikunze kugaragara cyane mu bana, bityo byitwa kandi polio. Virusi ya Polio iri mu bwoko bwa enteroviruse bwo mu muryango wa picornaviridae. Polioviruse yinjira mu mubiri w’umuntu kandi ikwirakwira cyane cyane mu nzira y’igogora. Ishobora kugabanywamo serotype eshatu hakurikijwe ubudahangarwa bw’umubiri, ubwoko bwa I, ubwoko bwa II, n’ubwoko bwa III.
Umuyoboro
| FAM | virusi ya polio yo mu bwoko bwa mbere |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 9 |
| Ubwoko bw'ingero | Icyitegererezo cy'imyenge mishya yakusanyijwe |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Kopi 1000/mL |
| Ibikoresho Bikoreshwa | Sisitemu ya PCR ikoreshwa mu gihe nyacyo ya 7500Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500QuantStudio®Sisitemu 5 za PCR mu gihe nyacyoSisitemu za PCR za SLAN-96P mu gihe nyacyo LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Amahitamo ya 1.
Ibikoresho byo gukuramo ibinyabutabire byasabwe: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (bishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Gukuramo ibinyabutabire bigomba gukorwa hakurikijwe IFU gusa. Ingano ya elution isabwa ni 80μL.
Amahitamo ya 2.
Ibikoresho byo gukuramo ibinyabutabire byasabwe: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3022) byakozwe na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Gukuramo ibinyabutabire bigomba gukorwa hakurikijwe IFU gusa. Ingano yo gukuramo ibinyabutabire yasabwe ni 100μL.
