Isesengura rya mbere ry’uburyo bwo gukurikirana imisemburo ya Brucella abortus mu Ntara ya Zhejiang rikorwa na gene yose - Itsinda rya Bioinformatics ryo mu bwoko bwa Macro & Micro-Test ryatanze umusanzu mu gutangaza amakuru ku banyamuryango

—— Ubushakashatsi ku bufatanye bwakozwe na Zhejiang CDC, Macro & Micro-Test, na CDC yo mu Bushinwa bwasohotse muri Frontiers in Cellular and Infection Microbiology

Incamake y'inyigo

Muri Gicurasi 2026, Frontiers in Cellular and Infection Microbiology (JCR Q1, IF ≈ 4.6) yasohoye inyandiko yayobowe n'Ikigo cy'Intara ya Zhejiang gishinzwe Kurwanya no Gukumira Indwara (Zhejiang CDC), hamwe n'itsinda ry'abahanga mu by'ibinyabuzima bo muri Beijing Macro & Micro-Test Bio-Tech Co., Ltd. n'Ikigo cy'Igihugu gishinzwe Kurwanya no Gukumira Indwara zandura (China CDC) nk'abanditsi bafatanyije. Ubu bushakashatsi bufite umutwe ugira uti:
"Isesengura ry'imiterere y'ubwoko bw'uturemangingo tw'ubwoko burindwi bwa Brucella abortus muri Zhejiang, mu Bushinwa."

Ubu bushakashatsi bugaragaza isesengura rya mbere ry’uburyo Brucella abortus (B. abortus) yakozwe mu buryo bukurikije imiterere ya jeni yose, rikozwe muri jeni yose, mu Ntara ya Zhejiang, mu Bushinwa. Itsinda ryasesenguye ubwoko bubiri bw’ibinyabuzima byakusanyijwe kuva mu 2015 kugeza mu 2025 (ubwoko bune bw’abantu n’ubwoko butatu bw’inka bukomoka muri Jinhua, Quzhou, na Ningbo). Ibyavuye mu bushakashatsi bitanga ibimenyetso bya jeni ku inkomoko n’inzira byakwirakwije iyi “nyoni yo mu majyaruguru” mu karere k’icyorezo kidasanzwe mu majyepfo y’uburasirazuba bw’Ubushinwa.

Amavu n'amavuko n'akamaro

Brucellosis ni indwara iterwa n’udukoko two mu bwoko bwa Brucella. Brucella abortus yanduza inka cyane cyane ariko ishobora no gutera indwara ku bantu. Mu Bushinwa, brucellosis igaragaza itandukaniro rikomeye mu turere: iyi ndwara ikunze kugaragara cyane mu ntara z’amajyaruguru (urugero, Inner Mongolia, Shanxi, Heilongjiang). Mu buryo bunyuranye, intara zo mu majyepfo, harimo na Zhejiang, zagiye ziganjemo Brucella melitensis, aho hagaragaye abantu bake cyane barwaye B. abortus. Iri tandukaniro mu turere rituma imiterere y’uturemangingo n’aho B. abortus ikomoka muri Zhejiang ari byo by’ingenzi mu buzima rusange.

Uburyo n'Ibyavuye mu bushakashatsi by'ingenzi

Itsinda ry’ubushakashatsi ryakoresheje ingamba nyinshi zihuza ibinyabuzima bya molekile na bioinformatics:

1.Kumenya indwara n'uburyo bw'ibanze bwo kwandika

BCSP-31 gene PCR na AMOS-PCR bemeje ko ubwoko bwose burindwi bw’uturemangingo ari B. abortus.

Ubwoko bwa Multilocus sequence type (MLST) bushingiye ku ngirabuzimafatizo icyenda zo mu rugo bwagaragaje ko ubwoko bwose bw’uturemangingo bwari ubwo mu bwoko bwa ST2, bigaragaza ko hari imiterere imwe y’uturemangingo mu bwoko bwa B. abortus buzenguruka muri Zhejiang.

2.Isesengura rya genome yose

Gupima genome yose byakozwe kuri platform ya Illumina NovaSeq. Isesengura ry’ikigereranyo cy’imiterere ya nucleotide (ANI) ryagaragaje ko Zhejiang isolates isa na 99.99% n’ubwoko bwa B. abortus 544.

Isesengura rya Pan-genome ryagaragaje ko hari umubare munini w’ibinyabuzima byarokotse: habonetse imiterere y’uturemangingo 3,084, hamwe n’uturemangingo 10 gusa tw’ibishishwa, kandi nta imiterere y’uturemangingo cyangwa imiterere y’ibicu yabonetse.

3.Imiterere y'uturemangingo tw'ubudahangarwa bw'indwara zandura n'imiti irwanya udukoko

Ibintu 68 bifitanye isano n’uburozi byahanuwe, bikubiyemo inzira za kera nka LPS biosynthesis, sisitemu yo gusohora T4SS, na sisitemu yo kugenzura BvrR-BvrS ifite ibice bibiri. Ikigaragara ni uko ubwoko bwose bw’uturemangingo bwabuze gene za adhesin bmaA na btaF. Isesengura rya gene ya resistance ryabonye gene ya mprF gusa muri CARD database, nta bindi bimenyetso bya resistance byagaragaye.

4. Ivugurura ry'imiterere y'umubiri no gukurikirana ubwandu bw'ibiyobyabwenge

Isesengura rya core-genome single-nucleotide polymorphism (cgSNP) ryashyize Zhejiang isolates ahantu runaka mu giti cy’ibinyabuzima ku isi. Ibisubizo byagaragaje ko ubwoko bwa Zhejiang bugize itsinda ry’ubwoko bumwe hamwe n’ubwoko bukomoka mu Burusiya, muri Mongoliya, no mu ntara nyinshi zo mu majyaruguru y’Ubushinwa (Ningxia, Heilongjiang, Inner Mongolia, Hebei, Gansu, Beijing). Iri tsinda ryigabanyijemo ibice bitatu bitandukanye (Clade 1-3), bigaragaza ko hari ibikorwa byinshi byigenga byo gutangiza.

Umwanzuro n'ingaruka

Ubu bushakashatsi butanga amakuru ya mbere yerekeye genome ya B. abortus mu Ntara ya Zhejiang kandi butanga imyanzuro myinshi y'ingenzi:

1. Clear inkomoko y'uturemangingo– Ubwoko bwa B. abortus buzenguruka i Zhejiang buri muri ST2, bukomeje kubikwa neza mu buryo bwa genome, kandi bugaragaza ubwoko busanzwe bwa brucellosis y'inka.

2. Eviurwego rw'ihererekanyamakuru ryambukiranya uturere– Isesengura rya phylogenetic ntirishyigikira ko hari ubwoko bwigenga bw’ibinyabuzima muri Zhejiang. Ahubwo, amakuru agaragaza ko ubwo bwoko bwaturutse mu majyaruguru y’Ubushinwa kandi ko bushobora kuba bufite inkomoko imwe mu miterere y’ibinyabuzima byo mu Burusiya na Mongoliya. Kuba hariho ibice bitatu by’ibinyabuzima by’ubwoko bumwe bigaragaza ko hari ibintu byinshi bitandukanye byabayeho.

3. Ingaruka ku buzima rusange– Ibyavuye mu bushakashatsi bigaragaza akamaro ko kugenzura indwara ya brucellosis no mu turere dusanzwe tutarakunze kugaragara nka Zhejiang. Nubwo umubare w’abarwaye ubu ari muto, ibikoresho bipima neza nka cgSNP bishobora gukurikirana neza inkomoko y’icyorezo cyaturutse mu mahanga no gutanga ibimenyetso bya siyansi mu guhagarika uruhererekane rw’ikwirakwizwa ry’indwara ziterwa n’ubwikorezi bw’amatungo hagati y’intara.

Uyu murimo ntuziba icyuho cy’ubushakashatsi mu Ntara ya Zhejiang gusa, ahubwo unatanga amakuru mashya y’ibanze yo kugenzura indwara ziterwa n’udukoko no gusuzuma ibyago bya brucellosis mu karere ka Yangtze River Delta.

Amakuru ku mpapuro:
Yang, Y., Shi, X., Chen, J., Wang, L., Wu, Z., Yao, W., … & Wu, B. (2026). Gusesengura no kumenya ubwoko bw'uturemangingo tw'ubwoko burindwi bwa Brucella abortus muri Zhejiang, mu Bushinwa. Frontiers in Cellular and Infection Microbiology, 16, 1758965.