ByoroshyeAmp by Macro & Micro Ikizamini —- Igikoresho cyitwa Portable Isothermal Fluorescence Amplification Igikoresho gihuye na LAMP / RPA / NASBA / HDA

Imikorere Neza & Porogaramu Yagutse

Byoroshye Amp, nubuhanga bwa isothermal nucleic aside amplificationni Byerekanwe na Hejuruibyiyumvo kandingufireactionigihe nta bisabwa uburyo bwo guhindura ubushyuhe. Kubwibyo, ifiteKugaragaraedNkabyinshigutonesha tekinoroji yo gupima aside nucleic yihuse.Nta bigoye tsisitemu yo gusiganwa ku magare, ibikoresho byoroheje kandi byoroshye isothermal amplification igikoreshoirashobora guhazainzira yose yo kubyitwaramo.

Macro & Micro-Ikizamini cyateye imbere mu bwigengehanyuma iratangizwaByoroshye,igikoresho cyitwa fluorescent isothermal amplification igikoreshoKuri Porogaramu zitandukanye.

Kuri ubuAmp byemewe kubabihujwe nubuhanga butandukanye bwo kongera ingufu za isothermal: Amplification ya Loop-Mediated Isothermal Amplification (LAMP), Amplification ya Recombinase Polymerase (RPA / RAA / MIRA), Nucleic Acide Sequence ishingiye kuri Amplification (NASBA), hamwe na Helicase Dependent Amplification (HDA).Bizaba ikindi gikoresho cyo kugerageza kwihuta kwa acide nucleic hamwe nibisabwa byinshi.

· Kwiyongera kwa Isothermal Amplification (LAMP):Kwiyongera kuri60-65 ° C.hamwe na byosereactioninzirabirangiye mu minota 30.Reba Ishusho ya 1 kugirango yongerwe imbaragaihame:

 

Igishushanyo 1. Ihameof Itara 

· Kwiyongera kwa Polymerase Polymerase (RPA / RAA / MIRA):Kwiyongera kuri37 ° C-42 ° C.hamwe na byosereactioninzirabirangiye mu minota 10-30.Reba Ishusho ya 2 kuri yoihame ryo kongera imbaraga:

Igishushanyo 2. Ihameof Recombinase Polymerase Amplification

·Nucleic Acide ikurikirana ishingiye kuri Amplification (NASBA):Kwiyongera kuri42 ° C.hamwe na byosereactioninzirabirangiye mu minota 40-120.Reba Ishusho ya 3 kuri yoihame ryo kongera imbaraga:

Igishushanyo 3. Ihameof Nucleic Acide ikurikirana ishingiye kuri Amplification

· Kwiyongera kwa Helicase Amplification (HDA):Kwiyongera kuri65 ° C.hamwe na byosereactioninzirabyuzuye mu minota 70-90.Reba Ishusho ya 4 kuri yoihame ryo kongera imbaraga:

Igishushanyo4.Ihameof Kwiyongera kwa Helicase

· EasyAmpGushobozas ikora neza kandi ihamye yo kumenya ADN na RNA ikoresheje tekinike enye zavuzwe haruguru.Igera kuri amplification sensibilité igera kuri 200 kopi / mL kandi ikanatanga umwihariko wo hejuru nta kwambukiranya intego.(Ibyerekanwe mu gishushanyo cya 5 kugeza ku cya 7).

Kumenya ADN Kumenya RNA

Igicapo.Ihuriro rya RAA / MIRA

Kumenya ADN Kumenya RNA

Igishushanyo6.Itara

 

Kumenya ADN Kumenya RNA

Igishushanyo7.Ihuriro rya NASBA

Ibyiza byibicuruzwa:

Byihuta

· EPIAikoranabuhanga rirabimenyaibisubizo byizabyagaragaye mu minota 5.

· Igikorwa cyoroshye hamwe no gukuramo intambwe imweyo kwifashisha igikoresho.

Bikora neza

· Module 4 yigenga kubisabwa, icyarimwe kugerageza 4 bitandukanyeubwoko bwikizaminis.

· Buri module ishoboye gusesengura icyarimwe icyarimwe.

· Dual / Quadfluorescenceimiyoborokuri byinshi-bigamije kumenya.

Guhuza

· Bihujwe nabyinshiurubuga rwa isothermal nka LAMP, RPA, NASBA, HDA.

· Yashizweho kugirango ubushyuhe bwo hejuru bwongerewe imbaraga nyuma yubushyuhe bwo hasi.

· Customizbashoboyekugirango uhuze abakiriya bakeneye.

Umukoresha-Nshuti

· P.Igishushanyo mbonera(290×245×128mm, 2kg) kuri Porogaramu zitandukanye.

· Ibara rya santimetero 7kubintu byoroshye byoroshye nibisubizo bifatika.

· Yubatswe muri printer yumuriro kugirango uhite usohora ibisubizo.

· Igikoresho gikomatanyirijwe hamwe cyo gusoma ibintu byikora.