Ikizamini cya EasyAmp cya Macro na Micro—Igikoresho cyo kongera imbaraga cya Isothermal Fluorescence gikoreshwa mu gutwara abantu n'ibintu gihuye n'itara/RPA/NASBA/HDA

Imikorere myiza cyane n'ikoreshwa ryagutse

Easy Amp, ikoresheje ikoranabuhanga rya ikongera aside nucleique y'ubushyuheigaragaramo byinshiuburyo bwo kumva nongufiuburyo bwo gusubizaigihe nta bisabwa kuri inzira yo guhindura ubushyuhe. Bityo rero, ifitegusohokaednk'ukohafi ya yoseikoranabuhanga ryakunze gukoreshwa mu gupima aside nucleique byihuse.Nta buryo bugoye bwo gukorasisitemu yo gutwara amagare y'ibimera, igikoresho cyoroshye kandi gishobora kwimurwa cyo kongera imbaraga mu bushyuheishobora guhazainzira yose yo gusubiza.

Macro & Micro-Test byateye imbere ku giti cyabyokandi yatangijweEasyAmp,igikoresho cyongera imbaraga gikoreshwa mu kuzamura ikirere gikoresha amashanyaraziku bikorwa bitandukanye.

Muri iki gihe,Amp yoroshye byemejwe ko ariijyanye n'uburyo butandukanye bwo kongera imbaraga mu bushyuhe: Loop-Mediated Isothermal Amplification (LAMP), Recombinase Polymerase Amplification (RPA/RAA/MIRA), Nucleic Acid Sequence Based Amplification (NASBA), na Helicase Dependent Amplification (HDA)Izaba ari ikindi gikoresho gigezweho cyo gupima aside nucleique byihuse kandi gikoreshwa mu buryo bwagutse cyane.

· Iterambere rya Isothermal rihuza urukiramende (ITARAMO):Amplification kuri60-65°Cbyose hamweuburyo bwo gusubizainzirabirarangiye mu minota 30.Kureba Ishusho ya 1 kugira ngo irusheho kwiyongeraihame:

 

Igishushanyo cya 1. Ihameof ITARA 

· Kugabanya ubushobozi bwo kongera polymerase (RPA/RAA/MIRA):Amplification kuri37°C-42°Cbyose hamweuburyo bwo gusubizainzirabirarangiye mu minota 10-30.Kureba Ishusho ya 2 kuri yoihame ryo kongera ubushobozi:

Igishushanyo cya 2. Ihameof Kongera ubushobozi bwa Recombinase Polymerase

·Ubushobozi bwo kongera imbaraga mu mikorere y'aside ya nucleike (NASBA):Amplification kuri42°Cbyose hamweuburyo bwo gusubizainzirabirarangiye mu minota 40-120.Kureba Ishusho ya 3 kuri yoihame ryo kongera ubushobozi:

Igishushanyo cya 3. Ihameof Iterambere rishingiye ku ruhererekane rw'aside ya nucleike

· Amplification Ishingiye ku Kajugujugu (HDA):Amplification kuri65°Cbyose hamweuburyo bwo gusubizainzirabirarangiye mu minota 70-90.Kureba Ishusho ya 4 kuri yoihame ryo kongera ubushobozi:

Igishushanyo cya 4. Ihameof Iterambere ry'Ibintu Bishingiye ku Kajugujugu

· EasyAmpshobozaIterambere ryiza kandi rihamye ryo gupima ADN na RNA hakoreshejwe uburyo bune bwa isothermal bwavuzwe haruguru. Igera ku bunararibonye bwo kongera ingufu kugeza kuri kopi 200/mL kandi ikagira ubushobozi bwo kugaragaza neza ibintu bitandukanye nta ngaruka mbi mu gutera imbaraga intego..(Ibigaragazwa mu Ishusho ya 5 kugeza kuri 7).

Gupima ADN Gupima RNA

Igishushanyo cya 5. Urubuga rwa RAA/MIRA

Gupima ADN Gupima RNA

Igishushanyo cya 6. Urubuga rw'amatara

 

Gupima ADN Gupima RNA

Igishushanyo cya 7. Urubuga rwa NASBA

Ibyiza by'ibicuruzwa:

Byihuse

· EPIAikoranabuhanga rigerahoigisubizo cyizabyabonetse mu minota 5.

· Gukora byoroshye hamwe no gukuramo intambwe imweyo gukoresha igikoresho.

Ikora neza

· Moduli 4 zigenga zo gupima icyarimwe ibintu 4 bitandukanye, igihe cyose bikeneweubwoko bw'ikizaminis.

· Buri module ishobora gusesengura ingero enye icyarimwe.

· Ibiri/Inefurumuriimiyoborokugira ngo hamenyekane mu buryo bwagutse ibintu byinshi.

Guhuza

· Ijyanye nabyinshiurubuga rwa isothermal nka LAMP, RPA, NASBA, HDA.

· Yagenewe kwandukura ubushyuhe bwinshi nyuma y'ubushyuhe buke.

· Guhinduragushoborakugira ngo habeho ibyo abakiriya bakeneye byihariye.

Biroroshye gukoresha

· Pigishushanyo mbonera cy'ameza(290×245×128mm, 2kg) kuri amasenari atandukanye yo gukoresha.

· Ecran yo mu bwoko bwa "touchscreen" ifite amabara ya santimetero 7kugira ngo byoroshye kuyikoresha kandi igaragaze neza umusaruro wayo.

· Imashini ikoresha ubushyuhe yubatswemo kugira ngo icapishe ibisubizo byihuse.

· Scanner yahujwe kugira ngo imenyekane mu buryo bwikora.