Gusuzuma BCR-ABL by'ingenzi mu kuvura CML

Indwara ya myelogenousleukemia idakira (CML) ni indwara mbi ifata uturemangingo tw’amaraso. Abarenga 95% by’abarwayi ba CML bafite chromosome ya Philadelphia (Ph) mu turemangingo tw’amaraso yabo. Kandi gene ya BCR-ABL fusion ikorwa no guhurira hagati ya ABL proto-oncogene (Abelson murine leukemia viral oncogene homolog 1) ku kuboko kure kwa chromosome 9 (9q34) na gene ya breakpoint cluster region (BCR) ku kuboko kure kwa chromosome 22 (22q11). Poroteyine yayo ya fusion ishingiye kuri iyi gene ifite imikorere ya tyrosine kinase (TK), kandi ikora inzira zayo zo mu nzira zo hasi (nka RAS, PI3K, na JAK/STAT) kugira ngo iteze imbere ukwigabanya kw’uturemangingo no gukumira apoptosis y’uturemangingo, bigatuma uturemangingo twiyongera cyane, bityo bigatuma CML ibaho.

Ingirabuzimafatizo za BCR-ABL, ingenzi mu iterambere rya CML, ni ikimenyetso cy'ingenzi cyo gusuzuma indwara. ya CML. Kuyimenya bifasha gukurikirana umurwayi'uburyo bwo gusubiza imiti igabanya ubukana bwa tyrosine kinase inhibitors (TKIs) kandi bugasobanura ko kanseri y'amaraso ishobora kongera kugaruka, ibyo bikaba ari ingenzi mu kuvura neza abarwayi ba CML.

BCR-ABL y'umuntuKit yo Gusuzuma Impinduka za Gene Fusionkuva kuri Macro na Micro-Test kugira ngo hamenyekane neza BCR-ABL mu barwayi ba CML, ifasha mu gusuzuma CML kandi ikayobora ubuvuzi bugamije kuvura kunoza ingamba zo kuvura kugira ngo umurwayi avurwe neza. BCR-ABL fusion gene, icyoniingenzimu iterambere rya CML,ikora nk'ikimenyetso cy'ingenzi cy'isuzumaofCMLKuyimenya bifashaumugenzuziumurwayi'sigisubizo ku miti igabanya ubukana bwa tyrosine kinase (TKIs)naiteganya ko kanseri y'amaraso ishobora kongera kugaruka, ari byongombwato ngirakamaroCMLimicungire y'abarwayi.

Kit yo Gusuzuma Impinduka za Gene za BCR-ABL zo mu Mutwe w'Abantu kuva kuri Macro na Micro-Test kugira ngo hamenyekane neza BCR-ABL mu barwayi ba CML, ubufashaCMLgusuzuma indwaranaabayoboziubuvuzi bw'ibanzetooptimizeingamba zo kuvura abarwayi neza.

ByuzuyeDgushakisha:Guhurira icyarimwegutahurakuri3gene zisanzwe zo guhuza (P210, P190, P230)kurigukwirakwiza amakuru menshi nokwirinda gucibwa imanza;

Ubushobozi bwo kugaragaza ibyiyumvo (sensitivity) buri hejuru:Verisiyo y'amazi isuzuma imiterere y'uturemangingo tw'ingirabuzimafatizohamwe na LoDy'amakopi 1000/mL,na verisiyo ya lyophilizedof Kopi 500/mL;

Byiza cyaneAukuri: Iimberekugenzurana enzyme ya UNG kugira ngo hirindwe ko hari ibimenyetso bitari byo& ibibi;

Gukoresha neza no gukora neza:Imikorere y'imiyoboro ifunze,nta bicuruzwa bifitenyuma yo gutunganya,nagutahuraimbereIminota 60kuriibisubizo bifatika kandi byizewe;

IbindiAmahitamo:Ubwoko bw'amazi n'ubw'amavuta ya lyophilized ku mahitamo y'abakiriya;

Guhuza neza:Ikorana na sisitemu nyinshi za PCR kugira ngo ikoreshwe muri laboratwari nyinshi.