Mycobacterium tuberculose acide na rifampicicin

Ibisobanuro bigufi:

Ibi bikoresho birakwiriye gutahura igituntu cya Mycobacterium kuri prutum ya muntu muri vitro, kimwe no mu karere ka codon muri 507-533 Amino amide itera igituntu cya RPOB irwanya.


Ibisobanuro birambuye

Ibicuruzwa

Izina ry'ibicuruzwa

HWTS-RT074B-Mycobacterium Igituntu Igituntu acide acide na rifampicicin staterst acide acide (guhuza umurongo)

Icyemezo

CE

Epidemiology

Mycobacterium Igituntu, Vuba, igituntu, igituntu, igituba, ni bagiteri ya pathogenic itera igituntu. Kugeza ubu, bikunze gukoreshwa umurongo wa mbere-umurongo urwanya ibinumbanyi birimo isoniazid, rifampicin na hexamubuLose, ibiyobyabwenge byateguwe ni umurongo wa kabiri. Ibiyobyabwenge bishya byateguwe ni Umurongo . Ariko, kubera gukoresha nabi Ubuvuzi bwa Mycobacteum burwanya ibinure nibiranga urukuta rwa Mycobacterium, MycobaCerium yo kurwanya ibiyobyabwenge, Ibiyobyabwenge bya MyCobaCose, ibiyobyabwenge bya Tuberculose, bizana ibibazo bikomeye gukumira no kuvura igituntu.

Rifampicin yakoreshejwe cyane mu kuvura abarwayi ba Pulmonar kuva mu mpera za za 70, kandi ifite ingaruka zikomeye. Nibwo buryo bwa mbere bwo kugabanya umuswa ya chimiotherculose abarwayi. Kurwanya Rifampicin biterwa ahanini biterwa no gutahura gene ya RPOB. Nubwo imiti mishya yo kurwanya ibinutu zihora zisohoka, kandi ivuriro ryabarwayi igituntu cya Pulmonary nazo zakomeje kandi kubura ibiyobyabwenge birwanya ibinure, kandi ibintu byo gukoresha ibiyobyabwenge bidashyira mu gaciro mu kuvura. Ikigaragara ni uko igituntu cya Mycobacterium ku barwayi bafite igituntu cy'indwara zijimye ntigishobora kwicwa mu gihe gikwiye, amaherezo kiganisha ku rwego rwo kurwanya ibiyobyabwenge mu mubiri w'umurwayi, utera inzira y'indwara, kandi yongera ibyago byo gupfa k'umurwayi.

Umuyoboro

Umuyoboro

Imiyoboro na fluorophores

Reaction buffer a

Buffer b

Reaction buffer c

Umuyoboro wa Fam

Umunyamakuru: Fam, Quentcher: Ntayo

RPOB 507-514

RPOB 513-520

38kd kandi ni6110

Umuyoboro wa Cy5

Umunyamakuru: Cy5, Quentcher: Ntayo

RPOB 520-527

RPOB 527-533

/

Umuyoboro wa hex (vic)

Umunyamakuru: Hex (Vic), Quntcher: Ntayo

Kugenzura imbere

Kugenzura imbere

Kugenzura imbere

Tekinike

Ububiko

≤-18 ℃ mu mwijima

Imibereho-Ubuzima

Amezi 12

Ubwoko bw'ikigereranyo

Sputum

CV

≤5.0%

Lod

mycobacterium igituntu 50 bagiteri / ml

RIFAMMINIC-Ubwoko bwo mu gasozi burwanya: 2x103bagiteri / ml

Mutant yo muke: 2x103bagiteri / ml

Umwihariko

Ivuga ubwoko-bwishyamba igituntu mycobacterium hamwe nuruhererekane rwibindi genes zo kurwanya ibiyobyabwenge nka Katg

Ibikoresho bisabwa:

Scan-96p sisitemu nyayo pcr sisitemu

Biorad CFX96 Igihe cya PCR

UmucyoR480® Igihe Cyiza PCR sisitemu

Akazi

Niba ukoresha Macro & Micro-Ikizamini Rusange ADN / RNA Kit (HWTS-3019-50, HWTS-3019-42, HWTS-3019-96) (Birashobora gukoreshwa hamwe na macro & micro-ikizamini cyikora Inkomoko ya Nucleic (HWTS-3006C, HWTS-3006B)) cyangwa Macro & micro-ikizamini cya virusi ya virusi / rna Inkingi (HWTS-3022-50) na Jiagsu Macro & Micro-Ikizamini Med-Tech Compaction yo kugenzura neza, nongeraho icyitegererezo cyiza kugirango ugeragezwe. Igenzura ryimbere muburyo bwo kugenzura neza, kugenzura nabi no kudurumba amabwiriza. Umuyoboro w'icyitegererezo wakuweho ni 200μl, hamwe n'ijwi risabwa ni 100μl.


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