Mycobacterium Igituntu Rifampicin Kurwanya
Izina RY'IGICURUZWA
HWTS-RT074A-Mycobacterium Igituntu Rifampicin Kurwanya Kurwanya Ibikoresho (Fluorescence PCR)
Epidemiologiya
Rifampicin yakoreshejwe cyane mu kuvura abarwayi b'igituntu cy'ibihaha kuva mu mpera za za 70, kandi igira ingaruka zikomeye.Nibwo buryo bwa mbere bwo kugabanya chimiotherapie y’abarwayi b’igituntu.Kurwanya Rifampicin biterwa ahanini na mutation ya rpoB.Nubwo imiti mishya yo kurwanya igituntu ihora isohoka, kandi n’ubuvuzi bw’abarwayi b’igituntu cy’ibihaha nabwo bwakomeje gutera imbere, haracyari ikibazo cyo kubura imiti igabanya ubukana, kandi ikibazo cyo gukoresha ibiyobyabwenge bidafite ishingiro mu mavuriro ni kinini.Ikigaragara ni uko igituntu cya Mycobacterium ku barwayi bafite igituntu cy'igituntu kidashobora kwicwa mu gihe gikwiye, amaherezo kikaba kiganisha ku ntera zitandukanye zo kurwanya ibiyobyabwenge mu mubiri w'umurwayi, bikongera inzira y'indwara, kandi byongera ibyago byo gupfa k'umurwayi.Iki gikoresho gikwiranye no gusuzuma indwara zifasha indwara ya Mycobacterium igituntu no kumenya gene irwanya rifampicin, ifasha mu gusobanukirwa imiti igabanya ubukana bwa mycobacterium yanduye abarwayi, no gutanga uburyo bufasha bwo kuyobora imiti ivura.
Epidemiologiya
Izina ry'intego | Umunyamakuru | Quencher | ||
BufferA | BufferB | BufferC | ||
rpoB 507-514 | rpoB 513-520 | IS6110 | FAM | Nta na kimwe |
rpoB 520-527 | rpoB 527-533 | / | CY5 | Nta na kimwe |
/ | / | Kugenzura imbere | HEX (VIC) | Nta na kimwe |
Ibipimo bya tekiniki
Ububiko | ≤-18 ℃ Mu mwijima |
Ubuzima bwa Shelf | Amezi 9 |
Ubwoko bw'icyitegererezo | Amacandwe |
CV | < 5 % |
LoD | ubwoko bwa rifampicin-bwihanganira ubwoko: 2x103bagiteri / mL mutant bahuje igitsina: 2x103bagiteri / mL |
Umwihariko | Itahura ubwoko bwigituntu cyitwa mycobacterium igituntu hamwe na mutation yizindi genes zirwanya ibiyobyabwenge nka katG 315G> C \ A, InhA-15C> T, ibisubizo byikizamini byerekana ko bitarwanya rifampicine, bivuze ko nta reaction-cross-reaction. |
Ibikoresho bikoreshwa: | SLAN-96P Sisitemu nyayo-PCR (Hongshi Medical Technology Co., Ltd.) |