Mycobacterium Igituntu Nucleic Acide na Rifampicin (RIF) ， Kurwanya Isoniazid (INH)

Mycobacterium Igituntu Nucleic Acide na Rifampicin (RIF) ， Isoniazid Resistance (INH) Ishusho Yerekanwe

Ibisobanuro bigufi:

Iki gicuruzwa gikwiranye no kumenya neza ADN ya Mycobacterium igituntu mu ngero z’ibibyimba by’abantu muri vitro, ndetse na mutation ya homozygous mu karere ka codon ya 507-533 ya aminide acide (81bp, rifampicin irwanya akarere) gene ya rpoB itera igituntu cya Mycobacterium igituntu. rifampicin.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-RT147 Mycobacterium Igituntu Nucleic Acide na Rifampicin (RIF), Isoniazid Resistance (INH) Igikoresho cyo gutahura (Gushonga umurongo)

Epidemiologiya

Mycobacterium igituntu, mugihe gito nka Tubercle bacillus (TB), ni bagiteri itera indwara yigituntu.Kugeza ubu, imiti ikoreshwa cyane ku murongo wa mbere urwanya igituntu harimo isoniazid, rifampicin na ethambutol, n'ibindi. Imiti yo mu murongo wa kabiri irwanya igituntu irimo fluoroquinolone, amikacin na kanamycin, n'ibindi. . Icyakora, kubera gukoresha nabi imiti irwanya igituntu hamwe nibiranga imiterere y'urukuta rw'akagari ka mycobacterium igituntu, mycobacterium igituntu itera imiti igabanya imiti igabanya ubukana, izana ibibazo bikomeye mu gukumira no kuvura igituntu.

Umuyoboro

Izina ry'intego			Umunyamakuru	Quencher
BufferA	BufferB	BufferC	Umunyamakuru	Quencher
rpoB 507-514	rpoB 513-520	IS6110	FAM	Nta na kimwe
rpoB 520-527	rpoB 527-533	/	CY5	Nta na kimwe
/	/	Kugenzura imbere	HEX (VIC)	Nta na kimwe

BufferD	Umunyamakuru	Quencher
InhA iteza imbere akarere -15C> T, -8T> A, -8T> C.	FAM	Nta na kimwe
KatG 315 codon 315G> A.、315G> C.	CY5	Nta na kimwe
AhpC iteza imbere akarere -12C> T, -6G> A.	ROX	Nta na kimwe

Ibipimo bya tekiniki

Ububiko	≤-18 ℃
Ubuzima bwa Shelf	Amezi 12
Ubwoko bw'icyitegererezo	ururenda
CV	≤5.0%
LoD	LoD ya mycobacterium igituntu yigihugu ni bagiteri 50 / mL.LoD ya rifampicin irwanya ubwoko bwishyamba ni 2 × 10³bagiteri / mL, na LoD y'ubwoko bwa mutant ni 2 × 10³bagiteri / mL.LoD ya bagiteri yo mu bwoko bwa isoniazid irwanya ni 2x10³bagiteri / mL, na LoD ya bagiteri ya mutant ni 2x10³bagiteri / mL.
Umwihariko	Ibisubizo by'ibizamini byambukiranya umusaraba byagaragaje ko nta reaction yakozwe mu gutahura genome y'abantu, izindi mycobacteria itari igituntu na virusi itera umusonga hamwe n'iki gikoresho;Nta reaction yabonetse yagaragaye ahahinduwe mutation yizindi genes zirwanya ibiyobyabwenge mubwoko bwa Mycobacterium igituntu.
Ibikoresho bikoreshwa	SLAN-96P Sisitemu nyayo-PCR (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Sisitemu nyayo-PCR Sisitemu, Ikoranabuhanga rya Hangzhou Bioer QuantGene 9600 Sisitemu nyayo-PCR, QuantStudio^®Sisitemu nyayo-PCR.