Mycobacterium Igituntu Nucleic Acide na Rifampicin (RIF) ， Kurwanya (INH)

Ibisobanuro bigufi:

Iki gikoresho gikoreshwa muri vitro yujuje ubuziranenge bwa Mycobacterium tuberculose ADN mumyanya yumuntu, umuco ukomeye (LJ Medium) numuco wamazi (MGIT Medium), amazi ya bronchial lavage, hamwe na mutation yo mukarere ka codon ya 507-533 (81bp, riffampicin irwanya akarere) gene ya rpoB hamwe na rincB ya tinine ya Mycobacteri. site ya mutation ya Mycobacterium tuberculose isoniazid irwanya.Bitanga ubufasha mugupima indwara ya Mycobacterium igituntu yanduye, kandi ikanagaragaza genes nyamukuru zirwanya rifampicin na isoniazid, ifasha kumva imiti irwanya Mycobacterium igituntu yanduye umurwayi.

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Ibicuruzwa birambuye

Ibicuruzwa

Izina ryibicuruzwa

HWTS-RT147 Mycobacterium Igituntu Nucleic Acide na Rifampicin (RIF), (INH) Igikoresho cyo gutahura (Gushonga umurongo)

Epidemiologiya

Mycobacterium tuberculose, mugihe gito nka Tubercle bacillus (TB), ni bagiteri itera indwara yigituntu, kandi kuri ubu, imiti ikoreshwa cyane kumurongo wambere urwanya igituntu harimo isoniazid, rifampicin na ethambutol, nibindi.^[1]. Icyakora, kubera gukoresha nabi imiti irwanya igituntu hamwe nibiranga imiterere y'urukuta rw'akagari ka mycobacterium igituntu ubwayo, igituntu cyitwa mycobacterium igituntu cyateje imbere imiti igabanya ubukana bw'igituntu, kandi uburyo buteye akaga ni imiti myinshi irwanya igituntu (MDR-TB), irwanya imiti ibiri ikunze kugaragara kandi ifite akamaro, rifampic.^[2].

Ikibazo cyo kurwanya igituntu kibaho mu bihugu byose byakoreweho ubushakashatsi na OMS. Kugirango hatangwe gahunda zukuri zo kuvura abarwayi b'igituntu, ni ngombwa kumenya kurwanya imiti igabanya ubukana, cyane cyane kurwanya rifampicine, ikaba yarabaye intambwe yo gusuzuma isabwa na OMS mu kuvura igituntu.^[3]. Nubwo kuvumbura imiti irwanya rifampicine bisa nkaho kuvumbura MDR-TB, gusa gutahura imiti irwanya rifampicin birengagiza abarwayi bafite INH irwanya mono (bivuga kurwanya isoniazid ariko ikumva rifampicine) na rifampicine irwanya mono (sensitivite kuri isoniazid ariko ikarwanya rifampicine), ibyo bikaba bishobora gutuma abarwayi bakorerwa uburyo bwambere bwo kuvura bidafite ishingiro. Kubwibyo, isoniazid na rifampicin kwipimisha nibisabwa byibuze muri gahunda zose zo kurwanya DR-TB^[⁴^].

Ibipimo bya tekiniki

Ububiko	≤-18 ℃
Ubuzima bwa Shelf	Amezi 12
Ubwoko bw'icyitegererezo	Urugero rwa Sputum, Umuco ukomeye (LJ Hagati), Umuco w'amazi (MGIT Medium)
CV	<5.0%
LoD	LoD yigikoresho cyo kumenya Mycobacterium igituntu ni bagiteri 10 / mL;LoD yigikoresho cyo kumenya rifampicin ubwoko bwishyamba nubwoko bwa mutant ni bagiteri 150 / mL; LoD yigikoresho cyo kumenya ubwoko bwa isoniazid nubwoko bwa mutant ni bacteri 200 / mL.
Umwihariko	1) Nta reaction yambukiranya mugihe ukoresheje ibikoresho kugirango umenye ADN genomic muntu (500ng), ubundi bwoko 28 bwindwara zubuhumekero, nubwoko 29 bwa mycobacteria idafite igituntu (nkuko bigaragara mumbonerahamwe 3).)3) Ibintu bisanzwe bibangamira ingero zigomba gupimwa, nka rifampicine (9mg / L), isoniazid (12mg / L), ethambutol (8mg / L), amoxicillin (11mg / L), oxymetazoline (1mg / L), mupirocin (20mg / L), pyrazinamide (45mg / L) (20mg / L) ibiyobyabwenge, nta ngaruka bigira kubisubizo byikizamini.
Ibikoresho bikoreshwa	SLAN-96P Sisitemu nyayo-PCR (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR