Indwara ya Mycobacterium Tuberculosis Nucleic Acid na Rifampicin (RIF)，Resistance (INH)

Ishusho ya Mycobacterium Tuberculosis Nucleic Acid na Rifampicin (RIF)，Resistance (INH)

Ibisobanuro bigufi:

Iyi kit ikoreshwa mu gupima ADN ya Mycobacterium tuberculosis mu maraso y'umuntu, gupima neza indwara (LJ Medium) no gupima amazi (MGIT Medium), gupima amazi yo mu bwonko, no kumenya impinduka mu gace ka aside amine 507-533 (agace ka 81bp, aho rifampicin irwanya) ya gene ya rpoB ya Mycobacterium tuberculosis rifampicin, ndetse no kumenya impinduka mu duce tw'ingenzi twa Mycobacterium tuberculosis isoniazid. Ifasha mu gusuzuma ubwandu bwa Mycobacterium tuberculosis, kandi ikamenya gene z'ingenzi za rifampicin na isoniazid, bifasha gusobanukirwa uburyo Mycobacterium tuberculosis yandura imiti.

Ohereza imeri kuri twe

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Izina ry'igicuruzwa

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid na Rifampicin (RIF), (INH) Detection Kit (Melting Curve)

Ubumenyi bw'indwara

Igituntu cya Mycobacterium, mu magambo make cyitwa Tubercle bacillus (TB), ni cyo gitera igituntu, kandi ubu, imiti ikoreshwa cyane mu kurwanya igituntu irimo isoniazid, rifampicin na ethambutol, nibindi.^[1]Ariko, bitewe n'ikoreshwa nabi ry'imiti irwanya igituntu ndetse n'imiterere y'urukuta rw'uturemangingo rwa mycobacterium tuberculosis ubwayo, mycobacterium tuberculosis yagize ubudahangarwa ku miti irwanya igituntu, kandi ubwoko buteje akaga cyane ni igituntu kidahangarwa ku miti myinshi (MDR-TB), kidahangarwa ku miti ibiri isanzwe kandi ikora neza, rifampicin na isoniazid.^[2].

Ikibazo cyo kudakira imiti y’igituntu kiri mu bihugu byose byasuzumwe na OMS. Kugira ngo haboneke gahunda nziza zo kuvura abarwayi ba gituntu, ni ngombwa kumenya kudakira imiti y’igituntu, cyane cyane kudakira kwa rifampicin, ikaba ari intambwe yashyizweho na OMS mu gusuzuma igituntu.^[3]Nubwo kuvumbura ubukana bwa rifampicin bisa nkaho ari ukuvumbura MDR-TB, gusa kumenya ubukana bwa rifampicin byirengagiza abarwayi bafite INH idashobora gufata imiti imwe (yerekeza ku bukana bwa isoniazid ariko ikaba ifite ubukana bwa rifampicin) na rifampicin idashobora gufata imiti imwe (ishobora gufata imiti imwe ariko ikaba ifite ubukana bwa rifampicin), ibyo bikaba bishobora gutuma abarwayi bakorerwa ubuvuzi bwa mbere butari ngombwa. Kubwibyo, ibizamini byo kwirinda kwa isoniazid na rifampicin ni ngombwa cyane muri gahunda zose zo kurwanya DR-TB.^[⁴^].

Ibipimo bya tekiniki

Ububiko	≤-18℃
Igihe cyo kumara	Amezi 12
Ubwoko bw'ingero	Urugero rw'amata, Umusaruro Ukomeye (LJ Medium), Umusaruro w'amazi (MGIT Medium)
CV	<5.0%
LoD	LoD y'agakoresho ko gupima igituntu cya Mycobacterium ni bagiteri 10/mL;LoD y'ibikoresho byo gupima ubwoko bwa rifampicin n'ubwoko bwa mutant ni bagiteri 150/mL; LoD y'ibikoresho byo gutahura ubwoko bw'inyamaswa bwa isoniazid n'ubwoko bwa mutant ni bagiteri 200/mL.
Ubwihariye	1) Nta buryo bwo guhuza ibintu buboneka iyo ukoresheje agakoresho kugira ngo umenye ADN y’uturemangingo tw’umubiri w’umuntu (500ng), ubundi bwoko 28 bw’udukoko two mu myanya y’ubuhumekero, n’ubwoko 29 bwa mycobacteria zitari iz’ibibyimba (nk’uko bigaragara mu mbonerahamwe ya 3).2) Nta buryo bwo guhuza ibintu buboneka iyo ukoresheje agakoresho kugira ngo umenye ahantu hahinduka mu tundi turemangingo twa rifampicin na Mycobacterium tuberculosis tutihanganira imiti (nk'uko bigaragara ku mbonerahamwe ya 4).3) Ibintu bikunze kwivanga mu bipimo bigomba gupimwa, nka rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), zanamivir (0.5mg/L), dexamethasone (20mg/L), nta ngaruka bigira ku bisubizo by'ikizamini cya kit.
Ibikoresho Bikoreshwa	Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.), Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo