Impinduka mu mikorere ya Mycobacterium Tuberculosis INH
Izina ry'igicuruzwa
HWTS-RT137 Mycobacterium Tuberculosis INH Mutation Detection Kit (Melting Curve)
Ubumenyi bw'indwara
Mycobacterium tuberculosis, mu magambo make yitwa Tubercle bacillus (TB), ni bagiteri itera indwara y'igituntu. Kuri ubu, imiti ikoreshwa cyane mu rwego rwa mbere irwanya igituntu irimo INH, rifampicin na hexambutol, nibindi. Imiti ikoreshwa mu rwego rwa kabiri irwanya igituntu irimo fluoroquinolones, amikacin na kanamycin, nibindi. Imiti mishya yakozwe ni linezolid, bedaquiline na delamani, nibindi. Ariko, bitewe n'ikoreshwa nabi ry'imiti irwanya igituntu n'imiterere y'urukuta rw'uturemangingo rwa mycobacterium tuberculosis, mycobacterium tuberculosis itera ubudahangarwa ku miti irwanya igituntu, ibi bikaba bitera imbogamizi zikomeye mu gukumira no kuvura igituntu.
Umuyoboro
| FAM | aside nucleique ya MP |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | inkorora |
| CV | ≤5% |
| LoD | Uruhare ntarengwa rwo kumenya bagiteri zo mu bwoko bwa INH zo mu gasozi ni bagiteri 2x103/mL, naho uruhare ntarengwa rwo kumenya bagiteri zahinduwe ni bagiteri 2x103/mL. |
| Ubwihariye | a. Nta buryo bwo guhuza imiterere y’uturemangingo tw’umubiri w’umuntu, izindi ndwara ziterwa na mikorobe n’indwara za pneumonia zigaragara muri aka gakoresho.b. Ahantu habayeho impinduka mu miterere y’utundi turemangingo turwanya imiti muri Mycobacterium tuberculosis yo mu bwoko bwa wild-type, nk’aho rifampicin rpoB gene irwanya ubudahangarwa, hagaragaye kandi ibisubizo by’ibizamini nta budahangarwa bwa INH, bigaragaza ko nta mpurirane zibaho. |
| Ibikoresho Bikoreshwa | Sisitemu za PCR za SLAN-96P mu gihe nyacyoSisitemu za PCR za BioRad CFX96 mu gihe nyacyoLightCycler480®Sisitemu ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Niba ukoresheje Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ya Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. kugira ngo ikuremo, ongeramo 200μL y'ingero za negative control n'iz'amase yatunganyijwe kugira ngo igeragezwe uko bikurikirana, hanyuma ongeramo 10μL y'ingero za internal control ukwayo mu ingero za negative control, izatunganywa izapimwa, kandi intambwe zikurikiraho zigomba gukorwa neza hakurikijwe amabwiriza yo gukuramo. Ingano y'ingero yakuwemo ni 200μL, kandi ingano ya elution isabwa ni 100μL.
