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    • Mycobacterium Igituntu INH Mutation

    Mycobacterium Igituntu INH Mutation Yerekanwe
    • Mycobacterium Igituntu INH Mutation

    Ibisobanuro bigufi:

    Iki gikoresho kirakwiriye kugirango hamenyekane neza ihinduka ry’ibanze ry’imiterere y’imiterere y’imyororokere y’abantu yakusanyirijwe mu barwayi beza ba Tubercle bacillus itera indwara ya mycobacterium igituntu INH: Akarere ka InhA gatera imbere -15C> T, -8T> A, -8T> C; AhpC iteza imbere akarere -12C> T, -6G> A; ihinduka ryimibonano mpuzabitsina ya KatG 315 codon 315G> A, 315G> C.


    Ibicuruzwa birambuye

    Ibicuruzwa

    Izina ryibicuruzwa

    HWTS-RT137 Mycobacterium Igituntu INH Igikoresho cyo Guhindura Mutation (Gushonga umurongo)

    Epidemiologiya

    Mycobacterium igituntu, mugihe gito nka Tubercle bacillus (TB), ni bagiteri itera indwara yigituntu. Kugeza ubu, imiti ikunze gukoreshwa ku murongo wa mbere urwanya igituntu harimo INH, rifampicin na hexambutol, n'ibindi. Imiti yo ku murongo wa kabiri irwanya igituntu irimo fluoroquinolone, amikacin na kanamycin, n'ibindi. igituntu, mycobacterium igituntu gitera kurwanya imiti igabanya ubukana, izana ibibazo bikomeye mu gukumira no kuvura igituntu.

    Umuyoboro

    FAM MP aside nucleic
    ROX

    Igenzura ryimbere

    Ibipimo bya tekiniki

    Ububiko

    ≤-18 ℃
    Ubuzima bwa Shelf Amezi 12
    Ubwoko bw'icyitegererezo ururenda
    CV ≤5%
    LoD Umupaka wo kumenya bacteri zo mu bwoko bwa INH ni bagiteri 2x103 / mL, naho igipimo cyo kumenya bagiteri za mutant ni 2x103 bagiteri / mL.
    Umwihariko a. Nta reaction yambukiranya genome yabantu, izindi mycobacteria nontuberculous mycobacteria na pneumonia patoggen zagaragajwe niki gitabo.b. Ahantu hagaragaye ihinduka ry’imiterere y’indi miti irwanya ibiyobyabwenge mu bwoko bw’igituntu cyitwa Mycobacterium igituntu, nk’imyigaragambyo igena akarere ka gene ya rifampicin rpoB, kandi ibisubizo by’ibizamini byagaragaje ko bitarwanya INH, byerekana ko nta musaraba wongeye gukora.
    Ibikoresho bikoreshwa SLAN-96P Sisitemu nyayo-PCRBioRad CFX96 Sisitemu Yigihe-PCRUmucyo®Sisitemu nyayo-PCR

    Urujya n'uruza rw'akazi

    Niba ukoresheje Macro & Micro-Ikizamini Rusange ADN / RNA Kit (HWTS-3019) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd kugirango ikurwemo, ongeramo 200μL yubushakashatsi bubi no gutunganyirizwa hamwe muburyo butandukanye. kugenzura, gutunganya intanga ngabo kugirango bipimwe, kandi intambwe ikurikiraho igomba gukorwa cyane ukurikije amabwiriza yo gukuramo. Ingano yakuweho ni 200μL, naho ingano yo gusabwa ni 100μL.

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