Mycobacterium igituntu nih mutation
Izina ry'ibicuruzwa
HWTS-RT137 Mycobacterium Igituro Igituntu Inh Mutation Kumenya (Gushonga umurongo)
Epidemiology
MycobaCerium Igituntu, Vuba nkadullcle bacillus (tb), ni bagiteri ya patteri zitera igituntu. Kugeza ubu, bikunze gukoreshwa umurongo wa mbere-umurongo urwanya ibinure kuri INH, RIFAMPICIn na HexamubuLose, nibindi bya kabiri byateye imbere ni umurongo wa kabiri. . Ariko, kubera gukoresha nabi ibiyobyabwenge byo kurwanya igituntu kandi Ibiranga urukuta rwakarere ka thectulose ya mycobacterium, MycobaCelose ya Mycobacterium, Mycobacterium itezimbere ibiyobyabwenge ibiyobyabwenge, ibiyobyabwenge bya Tuberculose, bizana ibibazo bikomeye gukumira no kuvura igituntu.
Umuyoboro
| Umuryango | Acide ya mpcleic |
| Rox | Kugenzura imbere |
Tekinike
| Ububiko | ≤-18 ℃ |
| Imibereho-Ubuzima | Amezi 12 |
| Ubwoko bw'ikigereranyo | sputum |
| CV | ≤5% |
| Lod | Umubare umenya uko-wanditseho mu gasozi Ish Bagiteri ni 2x103 bagiteri / ml, hamwe no kumenya imipaka ya bagito ya mutant ni 2x103 bagiteri / ml. |
| Umwihariko | a. Nta kunyura muri Genome ya muntu, izindi Nontntubercuus Mycobacteria na PNEOumonia pathogeneye yagaragaye niyi kikoresho.b. Imbuga zo gutemango zindi genes zirwanya ibiyobyabwenge muburyo bwa Mycobacterium ya ROWCOBicis RPOB, byagaragaye, kandi ibisubizo byikizamini ntibigaragaza ko irwanya IR, byerekana ko nta musaraba wasamye. |
| Ibikoresho bisabwa | Scan-96p sisitemu nyayo pcr sisitemuBiorad CFX96 Igihe cya PCRUmucyo 480®Sisitemu-nyayo pcr sisitemu |
Akazi
Niba ukoresha Macro & Micro-Ikizamini Rusange ADN / RNA Kit (HWTS-3019) (ikizamini cya acide cyikora) Med-Tech Co. Ongeramo 10μl yubuyobozi bwimbere muburyo bubi, icyitegererezo cya sputum kugirango ugeragezwe, kandi intambwe nyuma igomba gukorerwa neza ukurikije amabwiriza yo gukuramo. Umuyoboro w'icyitegererezo wakuweho ni 200μl, hamwe n'ijwi risabwa ni 100μl.
