Aside ya Nucleic ya MTHFR Gene Polymorphic
Izina ry'igicuruzwa
Kit yo Gupima Aside ya Nyukleyiki ya HWTS-GE004-MTHFR Gene Polymorphic Nucleic Acid (ARMS-PCR)
Ubumenyi bw'indwara
Aside folike ni vitamine ishongeshwa mu mazi, ikaba ari ingenzi mu mikorere y'umubiri. Mu myaka ya vuba aha, ubushakashatsi bwinshi bwemeje ko, impinduka ya gene ya folate metabolizing enzyme MTHFR izatuma habaho kubura aside folike mu mubiri, kandi kwangirika gusanzwe kw'ibura rya aside folike mu bantu bakuru bishobora gutera anemia ya megaloblastic, kwangirika kwa endothelium y'imitsi, nibindi. Kubura aside folike ku bagore batwite ntibishobora guhaza ibyifuzo byabo n'iby'umwana uri mu nda, bishobora gutera inenge z'imiyoboro y'ubwonko, anencephaly, impfu zo kubyara, no gukuramo inda. Urugero rwa folike mu maraso rugira ingaruka ku miterere ya 5,10-methylenetetrahydrofolate reductase (MTHFR). Impinduka za 677C>T na 1298A>C muri gene ya MTHFR zituma alanine ihinduka valine na glutamic acid, bityo bigatuma ikoreshwa rya MTHFR rigabanuka.
Umuyoboro
| FAM | MTHFR C677T |
| ROX | MTHFR A1298C |
| VIC (HEX) | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Amaraso aherutse gukusanywa ya EDTA arwanya amaraso |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1.0ng/μL |
| Ibikoresho Bikoreshwa: | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 Sisitemu za PCR za SLAN ®-96P mu gihe nyacyo Sisitemu 5 za PCR za QuantStudio™ mu gihe nyacyo Sisitemu ya LightCycler®480 Real-Time PCR Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Amahitamo ya 1
Ibikoresho byo gukuramo ibinyabutabire byasabwe: Kit ya DNA ya Macro & Micro-Test Genomic (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Amahitamo ya 2
Ibikoresho byo gukuramo ADN by’amaraso byasabwe: Kit yo gukuramo ADN y’amaraso (YDP348, JCXB20210062) ya Tiangen Biotech (Beijing) Co., Ltd. Kit yo gukuramo ADN y’amaraso (A1120) ya Promega.
