Mthfr Gene Polymorphic Nucleic Nucleic
Izina ry'ibicuruzwa
Hwts-ge004-mthfr gene Polymorphic nuclemprection acide imenyekanisha ibikoresho (amaboko-pcr)
Epidemiology
Acide folike ni vitamine ihujwe namazi ari ihuza ryingenzi mumihanda yumubiri. Mu myaka yashize, ubushakashatsi bwinshi bwo kwiga bwemeje ko, mutation ya folate yakanguye enzyme gene mtthf Ibyangiritse bya Endoteliya, nibindi bibura aside folike kubagore batwite badashobora kuzuza ibyo bakeneye no ku rubavu, rushobora Tera indero nshya yo mucyaro, anencephaly, kubyara, no gukuramo inda. Urwego rwa Serum rwakozwe na 5,10-methylenetetrahydhfolate igabanya kugabanya (Mthfr) Polymorphiism. 677C> na 1298a> Guhinduka muri Mthfr Gene yateye ihinduka rya Alanine kuri vine na aside ya glutamic, muburyo, bituma bigabanya ibikorwa bya MTHICR bityo bikagabanya imikoreshereze ya folike.
Umuyoboro
| Umuryango | Mthfr C677t |
| Rox | Mthfr A1298c |
| Vic (hex) | Kugenzura imbere |
Tekinike
| Ububiko | ≤-18 ℃ |
| Imibereho-Ubuzima | Amezi 12 |
| Ubwoko bw'ikigereranyo | Yakusanyije neza Edta anticoage mumaraso |
| CV | ≤5.0% |
| Ct | ≤38 |
| Lod | 1.0ng / μl |
| Ibikoresho bisabwa: | Gushyira mu bikorwa biosystems 7500 igihe cya sisitemu ya PCR Gushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Slan ®-96p Sisitemu nyayo PCR Quartudio ™ 5 PCR-Igihe cya PCR Umucyo®480 Igihe Cyigihe cya PCR Linegene 9600 wongeyeho sisitemu yigihe cya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Ihitamo 1
Basabwe gusubiramo .
Ihitamo 2
Basabwe gusubiramo reagents: amaraso ya dna ya dna ya dna (ydp348, jcxb20210062) na Tiangen biotech (Beijing) Co., Ltd. Amaraso yamarane yamaraso kit (a1120) na Promega.
