Acide Nucleic yo mu bwoko bwa Monkeypox Virus

Ishusho ya aside nucleic ya Monkeypox Virus Type

Ibisobanuro bigufi:

Iyi mashini ikoreshwa mu gupima virusi ya monkeypox clade I, aside nucleic clade II mu bipimo by'amazi y'ibihumyo mu maraso y'abantu, mu maraso no mu kanwa.

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HWTS-OT201Agakoresho ko Gupima Aside Nucleic mu Gukoresha Monkeypox Virus Type(Fluorescence PCR)

Ubumenyi bw'indwara

Monkeypox (Mpox) ni indwara yandura iterwa na virusi ya Monkeypox (MPXV). MPXV ifite ishusho y'uruziga cyangwa imeze nk'uruziga, kandi ni virusi ya ADN ifite imigozi ibiri ifite uburebure bwa Kb 197. Iyi ndwara ahanini yandurira mu nyamaswa, kandi abantu bashobora kwandura iyo barumwe n'inyamaswa zanduye cyangwa iyo bakoze ku maraso, amazi yo mu mubiri ndetse n'uduheri tw'inyamaswa zanduye. Iyi virusi ishobora kandi kwandurira mu bantu, cyane cyane binyuze mu bitonyanga by'ubuhumekero mu gihe cyo gukoranaho imbonankubone igihe kirekire cyangwa mu gukoranaho n'amazi yo mu mubiri w'umurwayi cyangwa ibintu byanduye. Ubushakashatsi bwagaragaje ko MPXV igizwe n'ibice bibiri bitandukanye: clade I (yari izwi nka clade yo muri Afurika yo Hagati cyangwa clade ya Congo Basin) na clade II (yari izwi mbere ko ari clade yo muri Afurika y'Iburengerazuba). Impox ya Congo Basin clade byagaragaye ko yandurira mu bantu kandi ishobora gutera urupfu, mu gihe impox ya Africa y'Iburengerazuba itera ibimenyetso byoroheje kandi ifite igipimo gito cyo kwandurira mu bantu kuva ku muntu kugera ku muntu.

Ibisubizo by'ibizamini by'iyi kit ntibigamije kuba ikimenyetso cyonyine cy'uko abarwayi banduye MPXV, bigomba guhuzwa n'imiterere y'umurwayi ndetse n'andi makuru y'ibizamini bya laboratwari kugira ngo hamenyekane neza ubwandu bw'iyi ndwara no gushyiraho gahunda ikwiye yo kuvura kugira ngo habeho ubuvuzi bwiza kandi butekanye.

Ibipimo bya tekiniki

Ubwoko bw'ingero	amazi y'ibisebe mu mubiri w'umuntu, agatambaro k'umuhogo n'amaraso
Ct	38
FAM	FAM-MPXV clade II VIC/HEX-MPXV clade I
CV	≤5.0%
LoD	Kopi 200/μL
Ubwihariye	Koresha agakoresho kugira ngo umenye izindi virusi, nka virusi ya Smallpox, virusi ya Cowpox, virusi ya Vaccinia, HSV1, HSV2, Virusi ya Herpes y'abantu ubwoko bwa 6, Virusi ya Herpes y'abantu ubwoko bwa 7, Virusi ya Herpes y'abantu ubwoko bwa 8, Measels vieus, Chicken pox-Herpes zoster virus, EB virus, Rubella virus n'ibindi, na nta gusubiza hamwe kugaragara.
Ibikoresho Bikoreshwa	Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 QuantStudio^®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo Sisitemu zo Gusuzuma za LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu za PCR za BioRad CFX96 mu gihe nyacyo Sisitemu za PCR za BioRad CFX Opus 96 mu gihe nyacyo