Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa hamwe n'udukoko twinshi turwanya imiti (KPC, NDM, OXA48 na IMP)
Izina ry'igicuruzwa
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa hamwe n'udukoko twinshi two kurwanya imiti (KPC, NDM, OXA48 na IMP) Multiplex Detection Kit (Fluorescence PCR)
Icyemezo
CE
Ubumenyi bw'indwara
Klebsiella pneumoniae ni indwara ikunze kwibasira abantu kandi ni imwe muri bagiteri zikomeye zitera indwara zandura mu bice bitandukanye by'umubiri. Iyo ubudahangarwa bw'umubiri bugabanutse, bagiteri zinjira mu bihaha ziva mu nzira y'ubuhumekero, bigatera ubwandu mu bice byinshi by'umubiri, kandi gukoresha imiti yica udukoko mu gihe cya vuba ni byo bivura [1]. Ahantu hakunze kugaragara ubwandu bwa Acinetobacter baumannii ni ibihaha, ari byo bitera indwara y'umusonga ifata ibitaro (HAP), cyane cyane umusonga ujyana n'umwuka (VAP). Akenshi ijyana n'izindi ndwara za bagiteri n'ibihumyo, ifite imiterere y'uburwayi bwinshi n'umubare munini w'impfu. Pseudomonas aeruginosa ni yo bacilli idatera ubwivumbure ikunze kugaragara cyane mu buvuzi, kandi ni yo itera indwara ifata abantu mu bitaro, ifite imiterere y'uko yoroshye kwibasira, ihindagurika ryoroheje kandi idashobora gukoresha imiti myinshi.
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Amasohoro |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | Kopi 1000/mL |
| Ubwihariye | a) Ikizamini cya "cross-reactivity" kigaragaza ko iyi kit idafite "cross reactivity" hamwe n'izindi ndwara zitera indwara zo mu myanya y'ubuhumekero, nka Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus na sputum samples zidafite intego, n'ibindi. b) Ubushobozi bwo kurwanya ingaruka mbi: Hitamo mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, aside clavulanic, na roxithromycin, nibindi kugira ngo upime ingaruka mbi, kandi ibisubizo bigaragaza ko ibintu bibangamira bivuzwe haruguru bitabangamira mu kubona Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa na carbapenem resistance genes KPC, NDM, OXA48 na IMP. |
| Ibikoresho Bikoreshwa | Porogaramu za mudasobwa zikoreshwa mu buryo bwa "Real-Time PCR Systems", Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500, QuantStudio®Sisitemu 5 za PCR zikora mu gihe nyacyo, LightCycler®Sisitemu ya PCR ya 480 ikora mu gihe nyacyo, Sisitemu yo Gupima PCR ya LineGene 9600 Plus mu gihe nyacyo (FQD-96A, ikoranabuhanga rya Bioer), MA-6000 Real-Time Quantitative Thermocycler (Suzhou Molarray Co., Ltd.), Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo, Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo. |
Urugendo rw'akazi
Kit ya Macro & Micro-Test General ADN/RNA (HWTS-3019) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) ya Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. niyo isabwa mu gukuramo icyitegererezo kandi intambwe zikurikiraho zigomba gukorwa hakurikijwe IFU ya Kit.
