Ibicurane A / B.
Izina RY'IGICURUZWA
HWTS-RT003A Ibicurane A / B Igikoresho cyo Kumenya Acide Nucleic (Fluorescence PCR)
Epidemiologiya
Indwara y'ibicurane A ni indwara yandurira mu myanya y'ubuhumekero, ifite ubwoko bwinshi nka H1N1 na H3N2, zikunda guhinduka kandi zikwira isi yose.Guhindura antigenic bivuga ihinduka rya virusi ya grippe A, bigatuma habaho ubwoko bushya.Virusi y'ibicurane B igabanyijemo ibice bibiri by'ingenzi, Yamagata na Victoria.Virusi ya grippe B ifite gusa antigenic drift, kandi birinda gukurikiranwa no gukuraho sisitemu y’umubiri y’umuntu binyuze mu ihinduka ry’imiterere.Nyamara, umuvuduko w’ubwihindurize wa virusi ya grippe B utinda cyane ugereranije na virusi ya grippe A, kandi virusi ya grippe B nayo ishobora gutera indwara zandurira mu myanya y'ubuhumekero kandi bigatera icyorezo.
Umuyoboro
| FAM | IFV A. |
| ROX | Igenzura ryimbere |
| VIC / HEX | IFV B. |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 9 |
| Ubwoko bw'icyitegererezo | oropharyngeal swab |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | IFV A ： 500Copi / mL ， IFV B ： 500Copi / mL |
| Umwihariko | 1 enterovirus, virusi ya parainfluenza, virusi ya mugiga, metapneumovirus yumuntu, virusi ya mumps, virusi ya syncytial virusi yo mu bwoko bwa B, rhinovirus, bordetella pertussis, chlamydia pneumoniae, corynebacterium, escherichia coli, haemophilus ibicurane, mactobacillus, jactobacill coplasma pneumoniae, neisseria meningitidis , neisseria gonorrhoeae, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, streptococcus pyogenes, streptococcus salivarius na ADN genomic ADN. 2. Ikizamini cyo kwivanga: Ibintu bivanga byatoranijwe nka mucin (60mg / mL), amaraso yumuntu, oxymetazoline (2mg / mL), sulfure (10%), beclomethasone (20mg / mL), dexamethasone (20mg / mL), flunisolide ( 20μg / mL), triamcinolone (2mg / mL), budesonide (1mg / mL), mometasone (2mg / mL), fluticasone (2mg / mL), benzocaine (10%), menthol (10%), zanamivir (20mg / mL ), azithromycine (1mg / L), cephalosporin (40μg / mL), mupirocine (20mg / mL), tobramycine (0,6mg / mL), fosifate oseltamivir (60ng / mL), ribavirin (10mg / L) ko ibintu bivanga hamwe nibitekerezo byavuzwe haruguru nta reaction ibangamira kumenya ibikoresho. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe-PCR Sisitemu Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR Sisitemu (Hongshi Medical Technology Co., Ltd.) Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu Yigihe-PCR yo Kumenya (FQD-96A, Ikoranabuhanga rya Hangzhou Bioer) MA-6000 Igihe Cyuzuye Cyumubare Wumukino Wamagare (Suzhou Molarray Co., Ltd.) BioRad CFX96 Sisitemu Yigihe-PCR na BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Ihitamo 1.
Gusabwa gukuramo reagent: Macro & Micro-Ikizamini rusange ADN / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa na Macro & Micro-Ikizamini Gukuramo Acide Nucleic Acide (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Gukuramo bigomba gukorwa hakurikijwe IFU.Ingano yo gukuramo urugero ni200μL.Ingano isabwa yo gukuraho ni 80μL.
Icya 2.
Basabwe gukuramo reagent: Macro & Micro-Ikigereranyo Cyitegererezo cyo Gusohora Reagent (HWTS-3005-8).Gukuramo bigomba gukorwa ukurikije IFU.
Icya 3.
Gusabwa gukuramo reagent: Gukuramo Acide Nucleic Acide cyangwa ibikoresho byoza (YDP315-R).Gukuramo bigomba gukorwa ukurikije IFU.Ingano yo gukuramo urugero ni 140μL.Ingano isabwa yo gukuraho ni 60μL.
