Impinduka mu mikorere ya za gene za ROS1 zo mu bwoko bwa Human
Izina ry'igicuruzwa
HWTS-TM009-Human ROS1 Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Icyemezo
CE
Ubumenyi bw'indwara
ROS1 ni tyrosine kinase yo mu bwoko bwa receptor ya insuline. Gene ya fusion ya ROS1 yemejwe nk'indi gene ikomeye itera kanseri y'ibihaha itari nto. Nk'ikimenyetso cy'ubwoko bushya bw'uturemangingo, ikwirakwira rya gene ya fusion ya ROS1 muri NSCLC Hagati ya 1% na 2% ROS1 ikorerwa ivugurura ry'uturemangingo muri exons zayo 32, 34, 35 na 36. Nyuma yo guhuzwa na gene nka CD74, EZR, SLC34A2, na SDC4, izakomeza gukora agace ka tyrosine kinase ya ROS1. PROS1 kinase ikora mu buryo budasanzwe ishobora gukora inzira zo mu nzira zo hasi nka RAS/MAPK/ERK, PI3K/Akt/mTOR, na JAK3/STAT3, bityo ikagira uruhare mu kwiyongera, gutandukanya no kwangirika kw'uturemangingo twa kanseri, no gutera kanseri. Mu mpinduka za ROS1, CD74-ROS1 ingana na 42%, EZR ingana na 15%, SLC34A2 ingana na 12%, naho SDC4 ingana na 7%. Ubushakashatsi bwagaragaje ko aho ATP-bonding domain ya ROS1 kinase ifatana na ATP hamwe n'aho ATP-bonding domain ya ALK kinase ifatana na ATP bifite homology ingana na 77%, bityo ALK tyrosine kinase small molecule inhibitor crizotinib n'ibindi bigira ingaruka zigaragara zo kuvura NSCLC hamwe na fusion mutation ya ROS1. Kubwibyo, kumenya ROS1 fusion mutations ni ishingiro n'ishingiro ryo kuyobora ikoreshwa ry'imiti ya crizotinib.
Umuyoboro
| FAM | Impuzandengo y'ingaruka 1, 2, 3 na 4 |
| VIC (HEX) | Impuguke mu gusubiza ibintu mu buryo bwa 4 |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 9 |
| Ubwoko bw'ingero | ingero z'ibinure by'indwara cyangwa ingero zaciwemo parafini |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | Iyi kit ishobora kubona impinduka za fusion kugeza kuri kopi 20. |
| Ibikoresho Bikoreshwa: | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 Sisitemu za PCR za SLAN ®-96P mu gihe nyacyo Sisitemu 5 za PCR za QuantStudio™ mu gihe nyacyo Sisitemu ya LightCycler®480 Real-Time PCR Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Reagent isabwa gukurwamo: RNeasy FFPE Kit (73504) yo muri QIAGEN, Paraffin Embedded Tissue Section Total RNA Extraction Kit (DP439) yo muri Tiangen Biotech (Beijing) Co., Ltd.
