Umuntu ROS1 Fusion Gene Mutation

Ibisobanuro bigufi:

Iki gikoresho gikoreshwa muburyo bwo kumenya vitro 14 yubwoko bwa ROS1 fusion gene ihindagurika mubantu ba kanseri y'ibihaha itari mito mito (Imbonerahamwe 1).Ibisubizo by'ibizamini ni ibyavuzwe gusa kandi ntibigomba gukoreshwa nk'ishingiro ryonyine ryo kuvura abarwayi ku giti cyabo.


Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-TM009-Umuntu ROS1 Fusion Gene Mutation Detection Kit (Fluorescence PCR)

Icyemezo

CE

Epidemiologiya

ROS1 ni transembrane tyrosine kinase yumuryango wa reseptor ya insuline.ROS1 fusion gene yemejwe nkindi gene yingenzi ya selile yibihaha ya kanseri y'ibihaha.Nkuhagarariye ubwoko bushya bwa molekuline idasanzwe, kwandura gene ya ROS1 muri NSCLC Hafi ya 1% kugeza 2% ROS1 ihura cyane na gene muri exons 32, 34, 35 na 36. Nyuma yo guhuzwa na gen nka CD74, EZR, SLC34A2, na SDC4, bizakomeza gukora akarere ka ROS1 tyrosine kinase.Gukora bidasanzwe ROS1 kinase irashobora gukora inzira yerekana ibimenyetso byerekana inzira nka RAS / MAPK / ERK, PI3K / Akt / mTOR, na JAK3 / STAT3, bityo ukagira uruhare mukwiyongera, gutandukanya na metastasis ya selile yibibyimba, no gutera kanseri.Muri mutation ya ROS1, CD74-ROS1 igera kuri 42%, EZR igera kuri 15%, SLC34A2 igera kuri 12%, naho SDC4 igera kuri 7%.Ubushakashatsi bwerekanye ko urubuga rwa ATP ruhuza catalitiki ya ROS1 kinase hamwe na ATP-ihuza urubuga rwa ALK kinase ifite homologiya igera kuri 77%, bityo ALK tyrosine kinase ntoya ya molekile inhibitor crizotinib nibindi bigira ingaruka zigaragara zo gukiza mukuvura NSCLC hamwe na fusion mutation ya ROS1.Kubwibyo, kumenya ihinduka rya ROS1 fusion ni ishingiro nishingiro ryo kuyobora ikoreshwa ryibiyobyabwenge bya crizotinib.

Umuyoboro

FAM Buffer reaction 1, 2, 3 na 4
VIC (HEX) Buffer reaction 4

Ibipimo bya tekiniki

Ububiko

≤-18 ℃

Ubuzima bwa Shelf

Amezi 9

Ubwoko bw'icyitegererezo

paraffin-yashyizwemo ingirabuzimafatizo cyangwa ingero zaciwe

CV

< 5.0%

Ct

≤38

LoD

Iki gikoresho gishobora kumenya ihinduka ryimiterere ya kopi 20.

Ibikoresho bikoreshwa:

Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCRIkoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR

SLAN ®-96P Sisitemu-Igihe nyacyo PCR

QuantStudio ™ 5 Sisitemu-Igihe nyacyo PCR

UmucyoCycler®480 Sisitemu nyayo-PCR

LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR

MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe

BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR

BioRad CFX Opus 96 Sisitemu Yigihe-PCR

Urujya n'uruza rw'akazi

Gusabwa gukuramo reagent: RNeasy FFPE Kit (73504) yo muri QIAGEN, Paraffin Yashizwemo Tissue Icyiciro Igice cyose cyo gukuramo RNA (DP439) cyo muri Tiangen Biotech (Beijing) Co., Ltd.


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