Umuntu ROS1 Fusion Gene Mutation
Izina RY'IGICURUZWA
HWTS-TM009-Umuntu ROS1 Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Icyemezo
CE
Epidemiologiya
ROS1 ni transembrane tyrosine kinase yumuryango wa reseptor ya insuline.ROS1 fusion gene yemejwe nkindi gene yingenzi ya selile yibihaha ya kanseri y'ibihaha.Nkuhagarariye ubwoko bushya bwa molekuline idasanzwe, kwandura gene ya ROS1 muri NSCLC Hafi ya 1% kugeza 2% ROS1 ihura cyane na gene muri exons 32, 34, 35 na 36. Nyuma yo guhuzwa na gen nka CD74, EZR, SLC34A2, na SDC4, bizakomeza gukora akarere ka ROS1 tyrosine kinase.Gukora bidasanzwe ROS1 kinase irashobora gukora inzira yerekana ibimenyetso byerekana inzira nka RAS / MAPK / ERK, PI3K / Akt / mTOR, na JAK3 / STAT3, bityo ukagira uruhare mukwiyongera, gutandukanya na metastasis ya selile yibibyimba, no gutera kanseri.Muri mutation ya ROS1, CD74-ROS1 igera kuri 42%, EZR igera kuri 15%, SLC34A2 igera kuri 12%, naho SDC4 igera kuri 7%.Ubushakashatsi bwerekanye ko urubuga rwa ATP ruhuza catalitiki ya ROS1 kinase hamwe na ATP-ihuza urubuga rwa ALK kinase ifite homologiya igera kuri 77%, bityo ALK tyrosine kinase ntoya ya molekile inhibitor crizotinib nibindi bigira ingaruka zigaragara zo gukiza mukuvura NSCLC hamwe na fusion mutation ya ROS1.Kubwibyo, kumenya ihinduka rya ROS1 fusion ni ishingiro nishingiro ryo kuyobora ikoreshwa ryibiyobyabwenge bya crizotinib.
Umuyoboro
FAM | Buffer reaction 1, 2, 3 na 4 |
VIC (HEX) | Buffer reaction 4 |
Ibipimo bya tekiniki
Ububiko | ≤-18 ℃ |
Ubuzima bwa Shelf | Amezi 9 |
Ubwoko bw'icyitegererezo | paraffin-yashyizwemo ingirabuzimafatizo cyangwa ingero zaciwe |
CV | < 5.0% |
Ct | ≤38 |
LoD | Iki gikoresho gishobora kumenya ihinduka ryimiterere ya kopi 20. |
Ibikoresho bikoreshwa: | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCRIkoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR SLAN ®-96P Sisitemu-Igihe nyacyo PCR QuantStudio ™ 5 Sisitemu-Igihe nyacyo PCR UmucyoCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Gusabwa gukuramo reagent: RNeasy FFPE Kit (73504) yo muri QIAGEN, Paraffin Yashizwemo Tissue Icyiciro Igice cyose cyo gukuramo RNA (DP439) cyo muri Tiangen Biotech (Beijing) Co., Ltd.