Umuntu Papillomavirus (Ubwoko 28) Genotyping
Izina ryibicuruzwa
HWTS-CC013-Papillomavirus Yabantu (Ubwoko 28) Igikoresho cyo Gutahura Genotyping (Fluorescence PCR)
HWTS-CC016A-Gukonjesha-byumye Papillomavirus Yumuntu (Ubwoko 28) Igikoresho cyo Gutahura Genotyping (Fluorescence PCR)
Epidemiologiya
Kanseri y'inkondo y'umura ni kimwe mu bibyimba bikunze kugaragara mu myanya myororokere y'abagore. Ubushakashatsi bwakozwe mbere bwerekanye ko kwandura no kwandura indwara nyinshi za papillomavirus ari imwe mu mpamvu zitera kanseri y'inkondo y'umura. Kugeza ubu, haracyari ikibazo cyo kuvura uburyo bwiza bwo kuvura HPV, bityo kumenya hakiri kare no kwirinda hakiri kare HPV y'inkondo y'umura ni urufunguzo rwo guhagarika kanseri. Gushiraho uburyo bworoshye, bwihariye kandi bwihuse bwo gusuzuma indwara ya etiologiya bifite akamaro kanini mugupima kanseri y'inkondo y'umura.
Umuyoboro
| Buffer reaction | FAM | VIC / HEX | ROX | CY5 |
| HPV Ubwoko bwa Genotyping reaction Buffer 1 | 16 | 18 | / | Kugenzura imbere |
| HPV Genotyping Reaction Buffer 2 | 56 | / | 31 | Kugenzura imbere |
| HPV Genotyping Reaction Buffer 3 | 58 | 33 | 66 | 35 |
| HPV Genotyping Reaction Buffer 4 | 53 | 51 | 52 | 45 |
| HPV Genotyping Reaction Buffer 5 | 73 | 59 | 39 | 68 |
| HPV Genotyping Reaction Buffer 6 | 6 | 11 | 83 | 54 |
| HPV Genotyping Reaction Buffer 7 | 26 | 44 | 61 | 81 |
| HPV Genotyping Reaction Buffer 8 | 40 | 43 | 42 | 82 |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | Inkondo y'umura Sw Igituba cy'inda ibyara 、 Inkari |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | 300Kopi / mL |
| umwihariko | Ibisubizo byose nibibi mugihe ibikoresho byakoreshejwe mugushakisha ingero zidasanzwe zifite reaction zambukiranya nayo, harimo ureaplasma urealyticum, chlamydia trachomatis yinzira yimyororokere, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella nubundi bwoko bwa HPV butapfukiranwa nigitabo. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe-PCR Sisitemu Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho-Igihe-Cyukuri cya PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Ihitamo 1.
Basabwe gukuramo reagent: Macro & Micro-Ikigereranyo Cyitegererezo cyo Kurekura Reagent (HWTS-3005-8)
Icya 2.
Basabwe gukuramo reagent: Macro & Micro-Test Virus ADN / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acide (HWTS-3006C, HWTS-3006B)
