Umuntu Methylated NDRG4 / SEPT9 / SFRP2 / BMP3 / SDC2 Gene
Izina RY'IGICURUZWA
HWTS-OT077-Umuntu Methylated NDRG4 / SEPT9 / SFRP2 / BMP3 / SDC2 Igikoresho cyo kumenya Gene (Fluorescence PCR)
Icyemezo
CE
Epidemiologiya
Ku bantu bakuru, ingirabuzimafatizo zirenga 10 8 zo mu mara zigwa ku rukuta rw'amara buri munsi, kandi zisohoka hamwe n'umwanda binyuze mu mara manini.Bitewe na selile yibibyimba birashoboka cyane kugwa mumyanya yo mara ikwirakwizwa ridasanzwe, intebe yabarwayi bo munda yibyimba byo munda irimo selile nyinshi zirwaye hamwe nibice bigize selile bidasanzwe, aribwo shingiro ryibikoresho byo gutahura neza intebe.Ubushakashatsi bwerekanye ko guhindura methylation ya porotokoro ya gene ari ibintu hakiri kare muri tumorigenez, kandi ibikoresho bikomoka ku ngirabuzima fatizo byakuwe mu ngero z'intebe z'abarwayi ba kanseri y'amara bishobora kwerekana ko kanseri iri mu mara hakiri kare.
NDRG4, izwi kandi ku izina rya SMAP-8 na BDM1, ni umwe mu bantu bane bagize umuryango wa NDRG gene (NDRG1-4), byagaragaye ko bifitanye isano no gukwirakwiza selile, gutandukana, iterambere no guhangayika.Byemejwe ko methylation ya NDRG4 ishobora kuba biomarker yo kudatera kanseri yibara kanseri yibara.
SEPT9 ni umwe mu bagize umuryango wa Septin gene, ugizwe nibura na gen zigera kuri 13 zigizwe na domeni ya GTPase yabitswe ishobora guhuza poroteyine ziterwa na cytoskeleton, kandi ifitanye isano no kugabana selile na tumorigenez.Ubushakashatsi bwerekanye ko gene ya methylated Septin9 yiyongera cyane mu byitegererezo by’intebe by’abarwayi barwaye kanseri yibara.
Intungamubiri zifunguye zifitanye isano na poroteyine (sFRPs) ni poroteyine zishonga zikaba icyiciro cya Wnt inzira ya antagonistes bitewe nuburyo bwabo bwo hejuru bwimiterere yabantu kuri reseptor (Fz) yakira ibimenyetso bya Wnt.Kudakora gene ya SFRP bivamo gukora bitagenzuwe na Wnt amarenga ajyanye na kanseri yibara.Kugeza ubu, methylation ya SFRP2 mu ntebe irashobora gukoreshwa nka biomarker idatera kugirango isuzume kanseri yibara.
BMP3 ni umunyamuryango wa TGF-B mu buryo budasanzwe bityo ikagira uruhare runini mu mikurire yo gusama itera no gushiraho amagufwa hakiri kare.BMP3 ni hypermethylated muri kanseri yibara kandi irashobora gukoreshwa nkikimenyetso cyingenzi cyibibyimba.
SDC2 ni selile ya heparan sulfate proteoglycan igira uruhare mugutunganya inzira nyinshi zifata umubiri na patologi.Gutunganyirizwa kumubiri harimo gukwirakwiza selile, gutandukanya, gufatira hamwe, cytoskeletal organisation, kwimuka, gukira ibikomere, itumanaho rya matrix, angiogenez;Inzira z'indwara zirimo gutwika na kanseri.Urwego rwa methylation ya SDC2 gene mubice bya kanseri yibara yari hejuru cyane ugereranije no mubice bisanzwe.
Umuyoboro
Buffer reaction A. | VIC / HEX | methylated NDRG4 gene |
ROX | methylated SEPT9 gene | |
CY5 | kugenzura imbere | |
Buffer reaction B. | VIC / HEX | methylated SFRP2 gene |
ROX | methylated BMP3 gene | |
FAM | methylated SDC2 gene | |
CY5 | kugenzura imbere |
Gusobanura
Gene | Umuyoboro | Ct Agaciro | Gusobanura |
NDRG4 | VIC (HEX) | Ct agaciro≤38 | NDRG4 nziza |
Ct agaciro> 38 cyangwa unde | NDRG4 nabi | ||
SEPT9 | ROX | Ct agaciro≤38 | SEPT9 nziza |
Ct agaciro> 38 cyangwa unde | SEPT9 nabi | ||
SFRP2 | VIC (HEX) | Ct agaciro≤38 | SFRP2 nziza |
Ct agaciro> 38 cyangwa unde | SFRP2 mbi |
Ibipimo bya tekiniki
Ububiko | Amazi: ≤-18 ℃ |
Ubuzima bwa Shelf | Amezi 9 |
Ubwoko bw'icyitegererezo | Icyitegererezo cy'intebe |
CV | ≤5.0% |
Umwihariko | Nta reaktivi ihura na kanseri y'umwijima, kanseri y'amara, kanseri ya tiroyide na kanseri y'ibihaha |
Ibikoresho bikoreshwa | QuantStudio ®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR UmucyoCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe |
Urujya n'uruza rw'akazi
Gusabwa gukuramo reagent: Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acide (HWTS- 3006).