Umuntu Methylated NDRG4 / SEPT9 / SFRP2 / BMP3 / SDC2 Gene

Ibisobanuro bigufi:

Igikoresho kigenewe muri vitro yujuje ubuziranenge bwa methylated NDRG4 / SEPT9 / SFRP2 / BMP3 / SDC2 ingirabuzimafatizo zo mu nda zanduye mu ngero z'umuntu.


Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-OT077-Umuntu Methylated NDRG4 / SEPT9 / SFRP2 / BMP3 / SDC2 Igikoresho cyo kumenya Gene (Fluorescence PCR)

Icyemezo

CE

Epidemiologiya

Ku bantu bakuru, ingirabuzimafatizo zirenga 10 8 zo mu mara zigwa ku rukuta rw'amara buri munsi, kandi zisohoka hamwe n'umwanda binyuze mu mara manini.Bitewe na selile yibibyimba birashoboka cyane kugwa mumyanya yo mara ikwirakwizwa ridasanzwe, intebe yabarwayi bo munda yibyimba byo munda irimo selile nyinshi zirwaye hamwe nibice bigize selile bidasanzwe, aribwo shingiro ryibikoresho byo gutahura neza intebe.Ubushakashatsi bwerekanye ko guhindura methylation ya porotokoro ya gene ari ibintu hakiri kare muri tumorigenez, kandi ibikoresho bikomoka ku ngirabuzima fatizo byakuwe mu ngero z'intebe z'abarwayi ba kanseri y'amara bishobora kwerekana ko kanseri iri mu mara hakiri kare.

NDRG4, izwi kandi ku izina rya SMAP-8 na BDM1, ni umwe mu bantu bane bagize umuryango wa NDRG gene (NDRG1-4), byagaragaye ko bifitanye isano no gukwirakwiza selile, gutandukana, iterambere no guhangayika.Byemejwe ko methylation ya NDRG4 ishobora kuba biomarker yo kudatera kanseri yibara kanseri yibara.

SEPT9 ni umwe mu bagize umuryango wa Septin gene, ugizwe nibura na gen zigera kuri 13 zigizwe na domeni ya GTPase yabitswe ishobora guhuza poroteyine ziterwa na cytoskeleton, kandi ifitanye isano no kugabana selile na tumorigenez.Ubushakashatsi bwerekanye ko gene ya methylated Septin9 yiyongera cyane mu byitegererezo by’intebe by’abarwayi barwaye kanseri yibara.

Intungamubiri zifunguye zifitanye isano na poroteyine (sFRPs) ni poroteyine zishonga zikaba icyiciro cya Wnt inzira ya antagonistes bitewe nuburyo bwabo bwo hejuru bwimiterere yabantu kuri reseptor (Fz) yakira ibimenyetso bya Wnt.Kudakora gene ya SFRP bivamo gukora bitagenzuwe na Wnt amarenga ajyanye na kanseri yibara.Kugeza ubu, methylation ya SFRP2 mu ntebe irashobora gukoreshwa nka biomarker idatera kugirango isuzume kanseri yibara.

BMP3 ni umunyamuryango wa TGF-B mu buryo budasanzwe bityo ikagira uruhare runini mu mikurire yo gusama itera no gushiraho amagufwa hakiri kare.BMP3 ni hypermethylated muri kanseri yibara kandi irashobora gukoreshwa nkikimenyetso cyingenzi cyibibyimba.

SDC2 ni selile ya heparan sulfate proteoglycan igira uruhare mugutunganya inzira nyinshi zifata umubiri na patologi.Gutunganyirizwa kumubiri harimo gukwirakwiza selile, gutandukanya, gufatira hamwe, cytoskeletal organisation, kwimuka, gukira ibikomere, itumanaho rya matrix, angiogenez;Inzira z'indwara zirimo gutwika na kanseri.Urwego rwa methylation ya SDC2 gene mubice bya kanseri yibara yari hejuru cyane ugereranije no mubice bisanzwe.

Umuyoboro

Buffer reaction A.

VIC / HEX methylated NDRG4 gene
ROX methylated SEPT9 gene
CY5 kugenzura imbere

Buffer reaction B.

VIC / HEX methylated SFRP2 gene
ROX methylated BMP3 gene
FAM methylated SDC2 gene
CY5 kugenzura imbere

Gusobanura

Gene

Umuyoboro

Ct Agaciro

Gusobanura

NDRG4

VIC (HEX)

Ct agaciro≤38

NDRG4 nziza

Ct agaciro> 38 cyangwa unde

NDRG4 nabi

SEPT9

ROX

Ct agaciro≤38

SEPT9 nziza

Ct agaciro> 38 cyangwa unde

SEPT9 nabi

SFRP2

VIC (HEX)

Ct agaciro≤38

SFRP2 nziza

Ct agaciro> 38 cyangwa unde

SFRP2 mbi

Ibipimo bya tekiniki

Ububiko Amazi: ≤-18 ℃
Ubuzima bwa Shelf Amezi 9
Ubwoko bw'icyitegererezo Icyitegererezo cy'intebe
CV ≤5.0%
Umwihariko Nta reaktivi ihura na kanseri y'umwijima, kanseri y'amara, kanseri ya tiroyide na kanseri y'ibihaha
Ibikoresho bikoreshwa QuantStudio ®5 Sisitemu nyayo-PCR

SLAN-96P Sisitemu nyayo-PCR

UmucyoCycler®480 Sisitemu nyayo-PCR

LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR

MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe

Urujya n'uruza rw'akazi

Gusabwa gukuramo reagent: Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acide (HWTS- 3006).


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