Umuntu Leukocyte Antigen B27 Acide Nucleic
Izina RY'IGICURUZWA
HWTS-GE011A-Umuntu Leukocyte Antigen B27 Igikoresho cyo Kumenya Acide Nucleic (Fluorescence PCR)
Epidemiologiya
Ankylose spondylitis (AS) n'indwara idakira itera indwara yibasira cyane urutirigongo kandi ishobora kuba irimo ingingo ya sacroiliac hamwe nibice bikikije kuburyo butandukanye.Byagaragaye ko AS igaragaza umuryango ugaragara kandi ko ifitanye isano rya bugufi na antigen ya leukocyte ya muntu HLA-B27.Mu bantu, ubwoko burenga 70 bwubwoko bwa HLA-B27 bwaravumbuwe kandi buramenyekana, kandi muribo, HLA-B * 2702, HLA-B * 2704 na HLA-B * 2705 nubwoko bukunze kugaragara bujyanye niyi ndwara.Mu Bushinwa, Singapuru, Ubuyapani n'akarere ka Tayiwani mu Bushinwa, ubwoko bukunze kugaragara bwa HLA-B27 ni HLA-B * 2704, bugera kuri 54%, bukurikirwa na HLA-B * 2705, bingana na 41%.Iki gikoresho gishobora kumenya ADN muburyo butandukanye HLA-B * 2702, HLA-B * 2704 na HLA-B * 2705, ariko ntibitandukanya.
Umuyoboro
FAM | HLA-B27 |
VIC / HEX | Igenzura ryimbere |
Ibipimo bya tekiniki
Ububiko | Amazi: ≤-18 ℃ Mu mwijima |
Ubuzima bwa Shelf | Amazi: amezi 18 |
Ubwoko bw'icyitegererezo | amaraso yose |
Ct | ≤40 |
CV | ≤5.0% |
LoD | 1ng / μL |
Umwihariko
| Ibisubizo by'ibizamini byabonetse muri iki gikoresho ntibizaterwa na hemoglobine (<800g / L), bilirubin (<700μmol / L), na lipide y'amaraso / triglyceride (<7mmol / L) mu maraso. |
Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems Intambwe imwe Yukuri-Igihe PCR Sisitemu UmucyoCycler®480 Sisitemu nyayo-PCR Agilent-Stratagene Mx3000P Q-PCR Sisitemu |