Impinduka mu miterere ya Gene 29 ya muntu (EGFR)
Izina ry'igicuruzwa
HWTS-TM0012A-Human EGFR Gene 29 Mutations Detection Kit (Fluorescence PCR)
Ubumenyi bw'indwara
Kanseri y'ibihaha yabaye intandaro ikomeye y'impfu za kanseri ku isi, ibangamira cyane ubuzima bw'abantu. Kanseri y'ibihaha itari iy'uturemangingo duto ifata hafi 80% by'abarwayi ba kanseri y'ibihaha. EGFR ni yo ntego ikomeye mu kuvura kanseri y'ibihaha itari iy'uturemangingo duto. Fosforili ya EGFR ishobora gutuma uturemangingo twa kanseri tugenda dukura, tukagira itandukaniro, tukagira ingaruka ku turemangingo, turwanya apoptosis, kandi ikanateza imbere angiogenesis ya tumor. EGFR tyrosine kinase inhibitors (TKI) ishobora kubuza inzira y'ibimenyetso bya EGFR ibuza autophosphorylation ya EGFR, bityo ikabuza ikwirakwira no gutandukana kw'uturemangingo twa tumor, ikanateza imbere apoptosis ya tumor cell, ikagabanya angiogenesis ya tumor, nibindi, kugira ngo hagerwe ku buvuzi bugamije tumor. Ubushakashatsi bwinshi bwagaragaje ko EGFR-TKI ivura neza ifitanye isano rya hafi n'imiterere y'impinduka mu ngirabuzimafatizo za EGFR, kandi ishobora kubuza by'umwihariko gukura kw'uturemangingo twa tumor hamwe n'impinduka mu ngirabuzimafatizo za EGFR. Ingirabuzimafatizo ya EGFR iherereye ku kuboko gugufi kwa chromosome 7 (7p12), ifite uburebure bwose bwa 200Kb kandi igizwe na exon 28. Agace kahinduwe gaherereye cyane cyane muri exon 18 kugeza 21, codons 746 kugeza 753 deletion mutation kuri exon 19 ingana na 45% naho L858R mutation kuri exon 21 ingana na 40% kugeza 45%. Amabwiriza ya NCCN yo gusuzuma no kuvura kanseri y'ibihaha itari iy'uturemangingo duto avuga neza ko gupima EGFR mutation ya gene ari ngombwa mbere yo guhabwa EGFR-TKI. Iyi mashini ikoreshwa mu kuyobora gutanga imiti ya epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), kandi igatanga ishingiro ry'imiti yihariye ku barwayi ba kanseri y'ibihaha itari iy'uturemangingo duto. Iyi mashini ikoreshwa gusa mu kumenya impinduka zisanzwe muri gene ya EGFR ku barwayi ba kanseri y'ibihaha itari iy'uturemangingo duto. Ibisubizo by'ibizamini ni ibyo kwifashisha mu buvuzi gusa kandi ntibigomba gukoreshwa nk'ishingiro ryonyine ryo kuvura abarwayi ku giti cyabo. Abaganga bagomba gusuzuma imiterere y'umurwayi, ibimenyetso by'imiti, n'uburyo avurwa. Uburyo yakiriye indwara n'ibindi bimenyetso by'ibizamini bya laboratwari n'ibindi bintu bikoreshwa mu gupima neza ibisubizo by'ibizamini.
Umuyoboro
| FAM | IC Reaction Buffer, L858R Reaction Buffer, 19del Reaction Buffer, T790M Reaction Buffer, G719X Reaction Buffer, 3Ins20 Reaction Buffer, L861Q Reaction Buffer, S768I Reaction Buffer |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18℃ Mu mwijima; Ifite impumuro mbi: ≤30℃ Mu mwijima |
| Igihe cyo kumara | Amazi: Amezi 9; Amavuta ya Lyophilized: Amezi 12 |
| Ubwoko bw'ingero | inyama nshya y'ikibyimba, igice cyakonjeshejwe cy'indwara, igice cyangwa igice cy'indwara cyashyizwemo parafini, plasma cyangwa serum |
| CV | <5.0% |
| LoD | Gupima umuti w’impinduka za aside nucleic munsi ya 3ng/μL wild-type, bishobora kumenya neza igipimo cya 1% cy’impinduka |
| Ubwihariye | Nta buryo bwo guhuza ADN y'umuntu yo mu bwoko bwa wild-type hamwe n'ubundi bwoko bwa mutant |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500Sisitemu za PCR zikoreshwa mu gihe nyacyo 7300 Sisitemu 5 za PCR za QuantStudio® mu gihe nyacyo Sisitemu ya LightCycler® 480 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
