Umuntu EGFR Gene 29 Guhinduka
Izina RY'IGICURUZWA
HWTS-TM001A-Umuntu EGFR Gene 29 Igikoresho cyo Guhindura Mutation (Fluorescence PCR)
Epidemiologiya
Kanseri y'ibihaha yabaye intandaro yo guhitanwa na kanseri ku isi hose, ibangamira cyane ubuzima bw'abantu.Kanseri y'ibihaha itari ntoya igizwe na 80% by'abarwayi ba kanseri y'ibihaha.Kugeza ubu EGFR niyo ntego ikomeye ya molekile yo kuvura kanseri y'ibihaha itari ntoya.Fosifora ya EGFR irashobora guteza imbere gukura kwingirangingo yibibyimba, gutandukanya, gutera, metastasis, anti-apoptose, no guteza imbere ikibyimba angiogenez.EGFR tyrosine kinase inhibitor (TKI) irashobora guhagarika inzira yerekana ibimenyetso bya EGFR muguhagarika autofosifora ya EGFR, bityo bikabuza ikwirakwizwa ryayo no gutandukanya ingirabuzimafatizo yibibyimba, guteza imbere kanseri yibibyimba apoptose, kugabanya ibibyimba angiogenezi, nibindi, kugirango bigere kubuvuzi bugamije kuvura ibibyimba.Umubare munini w’ubushakashatsi werekanye ko uburyo bwo kuvura EGFR-TKI bufitanye isano rya bugufi n’imiterere ya mutation ya EGFR, kandi bushobora kubuza cyane imikurire y’uturemangingo tw’ibibyimba hamwe na mutation ya EGFR.Gene ya EGFR iherereye ku kuboko kugufi kwa chromosome 7 (7p12), ifite uburebure bwuzuye bwa 200Kb kandi igizwe na exons 28.Agace kahinduwe gaherereye cyane cyane muri exons 18 kugeza 21, code 746 kugeza 753 yo gusiba mutation kuri exon 19 bingana na 45% naho ihinduka rya L858R kuri exon 21 rifite hafi 40% kugeza 45%.Amabwiriza ya NCCN yo gusuzuma no kuvura Kanseri y'ibihaha Atari Ntoya avuga neza ko hakenewe ibizamini bya mutation ya EGFR mbere y'ubuyobozi bwa EGFR-TKI.Iki gikoresho cyo kwipimisha gikoreshwa mu kuyobora imiyoborere yimiti ikura epidermal reseptor tyrosine kinase inhibitor (EGFR-TKI), kandi itanga umusingi wimiti yihariye kubarwayi bafite kanseri yibihaha itari mito.Iki gikoresho gikoreshwa gusa mugutahura ihinduka rusange muri gene ya EGFR kubarwayi barwaye kanseri y'ibihaha itari nto.Ibisubizo by'ibizamini ni ibyavuzwe gusa kandi ntibigomba gukoreshwa nk'ishingiro ryonyine ryo kuvura abarwayi ku giti cyabo.Abaganga b’amavuriro bagomba gusuzuma uko umurwayi ameze, ibimenyetso by’ibiyobyabwenge, n’ubuvuzi Igisubizo hamwe n’ibindi bipimo bya laboratoire hamwe n’ibindi bintu bikoreshwa mu gusuzuma byimazeyo ibyavuye mu kizamini.
Umuyoboro
| FAM | Buffer ya reaction ya L858R, Buffer ya reaction ya 19del, Buffer ya T790M, Buffer ya G719X, Buffer ya 3Ins20, Buffer ya LI861Q |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18 ℃ Mu mwijima;Lyophilized: ≤30 ℃ Mu mwijima |
| Ubuzima bwa Shelf | Amazi: amezi 9;Lyophilized: amezi 12 |
| Ubwoko bw'icyitegererezo | ibibyimba bishya byikibyimba, igice cyakonjeshejwe cya pathologiya, paraffin yashizwemo na patologue tissue cyangwa igice, plasma cyangwa serumu |
| CV | ＜ 5.0% |
| LoD | nucleic aside reaction ibisubizo byerekana inyuma ya 3ng / μL ubwoko bwishyamba, birashobora kumenya neza igipimo cya 1% |
| Umwihariko | Nta rezo-reaktivi ifite ubwoko bwa ADN genomic ya ADN nubundi bwoko bwa mutant |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCRIkoreshwa rya Biosystems 7300 Sisitemu-Igihe nyacyo PCR QuantStudio® 5 Sisitemu nyayo-PCR UmucyoCycler® 480 Sisitemu nyayo-PCR BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR |
Urujya n'uruza rw'akazi
