Ubuhanga bwa CYP2C9 mu bantu na VKORC1 mu kugabanya ingirabuzimafatizo
Izina ry'igicuruzwa
HWTS-GE014A-Human CYP2C9 na VKORC1 Gene Polymorphism Detection Kit (Fluorescence PCR)
Icyemezo
CE/TFDA
Ubumenyi bw'indwara
Warfarin ni umuti urwanya amaraso agabanuka ukoreshwa cyane mu buvuzi muri iki gihe, ugamije cyane cyane gukumira no kuvura indwara zo mu mitsi. Ariko, warfarin ifite igihe gito cyo kuvura kandi iratandukanye cyane mu moko atandukanye n'abantu ku giti cyabo. Imibare yagaragaje ko itandukaniro ry'ingano ihoraho mu bantu batandukanye rishobora kuba inshuro zirenga 20. Kuva amaraso mu buryo bubi bibaho kuri 15.2% by'abarwayi bafata warfarin buri mwaka, muri bo 3.5% bava amaraso menshi. Ubushakashatsi bwa Pharmacogenomique bwagaragaje ko polymorphism ya gene ya enzyme VKORC1 na enzyme metabolic CYP2C9 ya warfarin ari ikintu cy'ingenzi bigira ingaruka ku itandukaniro ry'ingano ya warfarin. Warfarin ni umuti wihariye ubuza vitamine K epoxide reductase (VKORC1), bityo ikabuza clotting factor synthesis irimo vitamine K kandi igatanga anticoagulant. Ubushakashatsi bwinshi bwagaragaje ko polymorphism ya gene ya VKORC1 promoter ari yo kintu cy'ingenzi bigira ingaruka ku bwoko n'itandukaniro ry'umuntu ku giti cye mu gipimo gisabwa cya warfarin. Warfarin ikorwa na CYP2C9, kandi impinduka zayo zitinda cyane mu mikorere ya warfarin. Abantu bakoresha warfarin bafite ibyago byinshi (byikubye kabiri kugeza kuri bitatu) byo kuva amaraso mu ntangiriro yo kuyikoresha.
Umuyoboro
| FAM | VKORC1 (-1639G>A) |
| CY5 | CYP2C9*3 |
| VIC/HEX | IC |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Amaraso mashya ya EDTA abuza amaraso gushonga |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Ubwihariye | Nta buryo bwo guhuza imiterere y’uturemangingo tw’umubiri hamwe n’izindi ngero zihoraho cyane za genome y’umuntu (gene ya CYP2C19 y’umuntu, gene ya RPN2 y’umuntu); impinduka za CYP2C9 * 13 na VKORC1 (3730G>A) zitari mu rwego rwo gupima iyi kit. |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 Sisitemu za PCR za QuantStudio®5 mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo Sisitemu ya LightCycler®480 Real-Time PCR Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Reagent isabwa gukurwamo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
