Polymorphism y'uturemangingo twa CYP2C19 tw'umuntu
Izina ry'igicuruzwa
HWTS-GE012A-Human CYP2C19 Gene Polymorphism Detection Kit (Fluorescence PCR)
Icyemezo
CE/TFDA
Ubumenyi bw'indwara
CYP2C19 ni imwe mu miti y'ingenzi ihindura imikorere y'imisemburo mu muryango wa CYP450. Imiterere myinshi y'ingirabuzimafatizo hamwe na hafi 2% by'imiti ivura ihinduka na CYP2C19, nko guhindura imikorere y'ingirabuzimafatizo zirwanya guterana kw'imisemburo (nka clopidogrel), ingirabuzimafatizo zigabanya umuvuduko w'amaraso (omeprazole), ingirabuzimafatizo zigabanya umuvuduko w'amaraso, nibindi. Polymorphisms za CYP2C19 zifite itandukaniro mu bushobozi bwo guhinduranya imisemburo y'imiti ifitanye isano. Izi mpinduka za *2 (rs4244285) na *3 (rs4986893) zitera kubura imikorere y'ingirabuzimafatizo ihinduka na gene ya CYP2C19 n'intege nke z'ubushobozi bwo guhinduranya imisemburo, kandi zikongera ubwinshi bw'amaraso, bityo bigatera ingaruka mbi ku miti ijyanye n'ubwinshi bw'amaraso. *17 (rs12248560) ishobora kongera imikorere y'ingirabuzimafatizo ihinduka na gene ya CYP2C19, gukora metabolites zikora, no kongera uburyo bwo gukumira guterana kw'imisemburo no kongera ibyago byo kuva amaraso. Ku bantu bafite ikibazo cyo gutinda k’imiti mu mubiri, gufata doze isanzwe igihe kirekire bitera uburozi bukomeye n’ingaruka mbi: cyane cyane kwangirika k’umwijima, kwangirika k’urugingo rw’amaraso, kwangirika k’urugingo rw’imitsi yo hagati, nibindi, bishobora gutera urupfu mu bihe bikomeye. Dukurikije itandukaniro ry’uburyo imiti ikoreshwa mu mubiri ikoreshwa mu mubiri, muri rusange igabanyijemo ibice bine, ari byo metabolism yihuta cyane (UM,*17/*17,*1/*17), metabolism yihuta cyane (RM,*1/*1), metabolism y’imitsi yo hagati (IM, *1/*2, *1/*3), metabolism igenda buhoro (PM, *2/*2, *2/*3, *3/*3).
Umuyoboro
| FAM | CYP2C19*2 |
| CY5 | CYP2C9*3 |
| ROX | CYP2C19*17 |
| VIC/HEX | IC |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Amaraso mashya ya EDTA abuza amaraso gushonga |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Ubwihariye | Nta buryo bwo guhuza ibintu bushoboka hamwe n’izindi gahunda zihoraho cyane (gene ya CYP2C9) muri genome y’umuntu. Impinduka za CYP2C19 * 23, CYP2C19 * 24 na CYP2C19 * 25 zitari mu rwego rwo kumenya ibi bipimo nta ngaruka zigira ku ngaruka zo kumenya ibi bipimo. |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 Sisitemu za PCR za QuantStudio®5 mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo Sisitemu ya LightCycler®480 Real-Time PCR Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Reagent isabwa gukurwamo: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Gukurwamo bigomba gukurwa hakurikijwe amabwiriza. Ingano y'icyitegererezo cyo gukurwamo ni 200μL, naho ingano y'icyitegererezo cyo gukurwamo ni 100μL.
Reagent yo gukuramo isabwa: Wizard® Genomic DNA Purification Kit (Catalog No.: A1120) na Promega, Nucleic Acid Extraction cyangwa Purification Reagent (YDP348) na Tiangen Biotech (Beijing) Co., Ltd. igomba gukurwamo hakurikijwe amabwiriza yo gukuramo, kandi ingano y’ikoreshwa ry ...
