Umuyoboro wa Hantaan
Izina ry'ibicuruzwa
Hwts-Fe005 Hantaan virusi ya acide nucleic no kumenya (fluorescence pcr)
Epidemiology
Hantavirus ni ubwoko bw'amahano, atandukanijwe, nabi-strand-strand-strand-strand. Hantagirus igabanijwemo ubwoko bubiri: umuntu atera Syndrome ya Hantavirus (HPS), undi atera Hantavirus Hemorrhagic umuriro hamwe na renal syndrome (hfrs). Ibyambere byiganje mu Burayi no muri Amerika, kandi ibya nyuma ni umuriro wa hemorrhagic ufite syndrome ya renal yatewe na virusi ya Hantaan, ikunze kugaragara mu Bushinwa. Ibimenyetso byubwoko bwa Hantavirus Hantaan cyane cyane cyane nkumuriro wa hemorhagic hamwe na syndrome ya renal, irangwa numuriro mwinshi, iryamana, Olpodiansienal, Oluria cyangwa Ibindi bikorwa byurugongo nimpamvu zidahwitse. Ni PAThogenic kubantu kandi igomba kwitabwaho bihagije.
Umuyoboro
| Umuryango | Ubwoko bwa Hantavirus Hantaan |
| Rox | Kugenzura imbere |
Tekinike
| Ububiko | ≤-18 ℃ |
| Imibereho-Ubuzima | Amezi 9 |
| Ubwoko bw'ikigereranyo | serum nshya |
| Ct | ≤38 |
| CV | <5.0% |
| Lod | Kopi 500 / μl |
| Ibikoresho bisabwa | Gukoresha Biosystems 7500 Igihe cya PCR Gushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Umutobe®5-igihe nyacyo PCR sisitemu Scan-96p sisitemu nyayo pcr sisitemu Umucyo®480 nyamara-sisitemu ya PCR Linegene 9600 wongeyeho sisitemu yigihe cya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Basabwe gusubiramo reagenct: Macro & Micro-Ikizamini cya virusi / RNA (HWTS-3017) Co., Ltd. Gukuramo bigomba gukorwa hakurikijwe Iyu. Gukuramo icyitegererezo ni 200μl. Umubumbe w'ikirenga usabwa ni 80μl.
Basabwe gusubiramo reagenct: gukuramo acide nucleic cyangwa ibikoresho byogusukura (ydp315-r). Gukuramo bigomba gukorwa hakurikijwe Iyu. Gukuramo icyitegererezo ni 140μl. Umubumbe wo hejuru wasabwe ni 60μl.
