Virusi ya encephalitis
Izina ry'ibicuruzwa
Hwts-Fe006 Ishyamba Encephalitis virusi virusi ya aside nucleti
Epidemiology
Ishyamba Encephalite (Fe), rizwi kandi ku izina rya Tick-Borne-BBENT, TBE), ni indwara ikaze ya sisitemu yo hagati yatewe na virusi ya Encephalite. Virusi ya Encephalitis Encephalitis ni iy'umuryango wa Flavidiridae. Ibice bya virusi ni diegical hamwe na diameter ya 40-50nm. Uburemere bwa molekiri ni 4 × 106Da, na virusi genome nuburyo bwiza, rna imwe[1]. Ubuvuzi, burangwa numuriro mwinshi, kubabara umutwe, coma, gutangira byihuse kurakara, hamwe nubumuga bwimitsi yijosi namaguru, kandi bifite igipimo cyimbaho. Gusuzumwa hakiri kare kandi byihuse virusi ya Encephalitis yo mu mashyamba nurufunguzo rwo kuvura amashyamba ya Encephalite yo mu mashyamba, kandi hashyizweho uburyo bworoshye, bwihariye kandi bwihuse kandi bwihuse kandi bwihuse kandi bwihuse kandi bwihuse kandi bwihuse mu buryo bukomeye mu gusuzuma kwa clinical ya encephalitis[1,2].
Umuyoboro
| Umuryango | Ishyamba Encephalitis virusi nucleic nucleic |
| Rox | Kugenzura imbere |
Tekinike
| Ububiko | ≤-18 ℃ |
| Imibereho-Ubuzima | Amezi 9 |
| Ubwoko bw'ikigereranyo | serum nshya |
| Tt | ≤38 |
| CV | ≤5.0% |
| Lod | 500copies / ml |
| Ibikoresho bisabwa | Gushyira mu bikorwa biosystems 7500 igihe cya sisitemu ya PCR Gushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Umutobe®5-igihe nyacyo PCR sisitemu Scan-96p sisitemu nyayo pcr sisitemu Umucyo®480 nyamara-sisitemu ya PCR Linegene 9600 wongeyeho sisitemu nyayo ya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Gusaba gukuramo reagent: gukuramo acide nucleic cyangwa isuku (ydp315-r) by tiijing biotech (Beijing) Co., LTD., Gukuramo) Co., Ltd. Icyitegererezo cyakuweho Umuyoboro w'icyitegererezo ni 140μl hamwe n'ijwi risabwa ni 60μl.
Basabwe gukuramo Reagent: Macro-Ikizamini Rusange ADN / RNA Kit (HWTS-3017-50, HWTS-3017-96) na micro-Ikizamini Automatic Automact Hwts-3006, hwts-3006c, hwts-3006b). Gukuramo bigomba gukorwa hakurikijwe amabwiriza. Icyitegererezo cyakuweho icyitegererezo ni 200μl hamwe nububiko bwabigenewe ni 80μl.
