Virusi ya Encephalitis
Izina ryibicuruzwa
HWTS-FE006 Ishyamba Encephalitis Virus Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Encephalitis yo mu mashyamba (FE), izwi kandi ku izina rya tick-borne encephalitis (Tick-borne encephalitis, TBE), ni indwara ikaze yandura ya sisitemu yo hagati yo hagati iterwa na virusi ya encephalitis. Virusi ya encephalitis yo mu mashyamba ni iy'ubwoko bwa Flavivirus yo mu muryango wa Flaviviridae. Ibice bya virusi ni serefegitire ya diameter ya 40-50nm. Uburemere bwa molekile ni 4 × 106Da, na virusi genome ni imyumvire-myiza, RNA imwe[1]. Mubuvuzi, burangwa no kugira umuriro mwinshi, kubabara umutwe, koma, gutangira vuba kurakara kwa meningeal, hamwe no kumugara imitsi yijosi n'amaguru, kandi bifite impfu nyinshi. Kwipimisha hakiri kare kandi byihuse virusi ya encephalitis yo mu mashyamba nurufunguzo rwo kuvura encephalite y’amashyamba, kandi gushyiraho uburyo bworoshye, bwihariye kandi bwihuse bwo gusuzuma indwara ya etiologiya bifite akamaro kanini mugupima ivuriro rya encephalite y’amashyamba[1,2].
Umuyoboro
| FAM | amashyamba encephalitis virusi nucleic aside |
| ROX | Igenzura ryimbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 9 |
| Ubwoko bw'icyitegererezo | serumu nshya |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500Copi / mL |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho-Igihe-Cyukuri cya PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Gusabwa gukuramo reagent: Gukuramo Acide Nucleic Acide cyangwa Isuku (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd., Gukuramo bigomba gukorwa hakurikijwe amabwiriza akomeye. Icyitegererezo cyakuweho icyitegererezo ni 140μL naho icyifuzo cyo gukuraho ni 60μL.
Gusabwa gukuramo reagent: Macro & Micro-Ikizamini Rusange ADN / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acide (HWTS-3006, HWTS-3006C, HWTS-3006B, HWTS-3006B, gukuramo bigomba gukorwa ukurikije amabwiriza akomeye. Icyifuzo cyakuweho urugero ni 200μL naho icyifuzo cyo gukuraho ni 80μL.
