Virusi ya Encephalite yo mu ishyamba
Izina ry'igicuruzwa
HWTS-FE006 Ipaki yo Gupima Aside Nucleic Virus ya Encephalitis yo mu Ishyamba (Fluorescence PCR)
Ubumenyi bw'indwara
Indwara yo mu ishyamba (FE), izwi kandi nka "tick-borne encephalitis" (tick-borne encephalitis, TBE), ni indwara yandura cyane mu mitsi yo hagati iterwa na virusi yo mu ishyamba. Virusi yo mu ishyamba ikomoka kuri "forester encephalitis" iri mu bwoko bwa Flavivirus yo mu muryango wa Flaviviridae. Uduce twa virusi dufite umurambararo wa 40-50nm. Uburemere bwa molekile ni hafi 4×106Da, kandi genome ya virusi ni RNA ifite imiterere myiza, ifite umurongo umwe[1]Mu buvuzi, irangwa n'umuriro mwinshi, kuribwa umutwe, koma, gushya vuba mu mitsi, no gucika intege kw'imitsi yo mu ijosi n'amaguru, kandi ifite igipimo cyo hejuru cy'impfu. Gusuzuma virusi ya encephalitis yo mu ishyamba hakiri kare kandi vuba ni ingenzi mu kuvura encephalitis yo mu ishyamba, kandi gushyiraho uburyo bworoshye, bwihariye kandi bwihuse bwo gusuzuma indwara ni ingenzi cyane mu gusuzuma encephalitis yo mu ishyamba.[1,2].
Umuyoboro
| FAM | aside nucleique ya virusi ya encephalitis yo mu ishyamba |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 9 |
| Ubwoko bw'ingero | serumu nshya |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Kopi 500/mL |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu zo Gusuzuma za LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Reagent isabwa gukurwamo: Kit yo gukuramo aside ya Nucleic cyangwa Purification (YDP315-R) ya Tiangen Biotech (Beijing) Co., Ltd., gukuramo bigomba gukorwa hakurikijwe amabwiriza neza. Ingano y'icyitegererezo cyakuwemo ni 140μL naho ingano y'icyitegererezo cyatanzwe ni 60μL.
Reagent isabwa gukurwamo: Macro & Micro-Test General ADN/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006, HWTS-3006C, HWTS-3006B). Gukurwamo bigomba gukorwa hakurikijwe amabwiriza neza. Ingano y'icyitegererezo cyakuwemo ni 200μL naho ingano y'icyitegererezo cyatanzwe ni 80μL.
