Ubwoko bwa virusi zubuhumekero
Izina RY'IGICURUZWA
HWTS-RT099- Ubwoko 4 bwa virusi zubuhumekero Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Indwara ya Corona Virusi 2019, yitwa "COVID-19", yerekeza ku musonga watewe2019-nCoVkwandura.2019-nCoVni coronavirus ya β ubwoko.COVID-19 ni indwara yandurira mu myanya y'ubuhumekero, kandi muri rusange abaturage bakunze kwibasirwa.Kugeza ubu, isoko yanduye ahanini ni abarwayi banduye2019-nCoV, kandi abantu banduye badafite ibimenyetso bashobora no kuba intandaro yo kwandura.Ukurikije iperereza ryakozwe muri iki gihe, igihe cyo gukuramo ni iminsi 1-14, cyane cyane iminsi 3-7.Umuriro, inkorora yumye n'umunaniro nibyo bigaragara.Abarwayi bake bari bafite ibimenyetsos nkaizuru ryizuru, izuru ritemba, kubabara mu muhogo, myalgia na diyare, n'ibindi.
Umuyoboro
FAM | 2019-nCoV |
VIC (HEX) | RSV |
CY5 | IFV A. |
ROX | IFV B. |
NED | Igenzura ryimbere |
Ibipimo bya tekiniki
Ububiko | -18 ℃ |
Ubuzima bwa Shelf | Amezi 9 |
Ubwoko bw'icyitegererezo | Oropharyngeal swab |
Ct | ≤38 |
LoD | 2019-nCoV: 300Copi / mLVirusi ya grippe A / virusi ya grippe B / virusi yubuhumekero: 500Copies / mL |
Umwihariko | a) Ibisubizo byambukiranya imipaka byerekana ko nta reaction ihari hagati yigitabo na coronavirus yumuntu SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, virusi ya parainfluenza ubwoko bwa 1, 2, 3. virusi, legionella, bordetella pertussis, ibicurane bya haemophilus, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculose, umwotsi aspergillus, candida albicans, candida albicans ic ac. b) Ubushobozi bwo kurwanya-kwivanga: hitamo mucin (60mg / mL), 10% (v / v) yamaraso na fenilephrine (2mg / mL), oxymetazoline (2mg / mL), chloride ya sodiumi (harimo na preservateurs) (20 mg / mL) ), beclomethasone (20mg / mL), dexamethasone (20mg / mL), flunisolide (20μg / mL), triamcinolone acetonide (2mg / mL), budesonide (2mg / mL), mometasone (2mg / mL), fluticasone (2mg / mL) ), histamine hydrochloride (5mg / mL), alpha interferon (800IU / mL), zanamivir (20mg / mL), ribavirin (10mg / mL), oseltamivir (60ng / mL), peramivir (1mg / mL), lopinavir (500mg / mL), ritonavir (60mg / mL), mupirocine (20mg / mL), azithromycine (1mg / mL), ceftriaxone (40μg / mL), meropenem (200mg / mL), levofloxacin (10μg / mL) na tobramycine (0,6mg / mL) kubizamini byo kwivanga, kandi ibisubizo byerekana ko ibintu bivanga hamwe nibitekerezo byavuzwe haruguru nta reaction ibangamira ibisubizo byikizamini cya virusi. |
Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe-PCR Sisitemu Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR |
Urujya n'uruza rw'akazi
Ihitamo 1.
Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006) yakozwe na Jiangsu Macro & Micro -Gupima Med-Tech Co, Ltd. Ikigereranyo cyakuweho ni 200μL, naho icyifuzo cyo gukuraho ni 80μL.
Icya 2.
QIAamp Viral RNA Mini Kit (52904) yakozwe na QIAGEN cyangwa Nucleic Acide Extraction cyangwa Isukura (YDP315-R) yakozwe na Tiangen Biotech (Beijing) Co, Ltd.