Ubwoko 15 bwo gutanga ibyago byinshi bya pepillomavirus E6 / E7 Gere Mhna

Ubwoko 15 bwo guhura nacyo cyane Papillomavirus E6 / E7 Gene MRNANA Yagaragaye Ishusho

Ibisobanuro bigufi:

Iyi kikoresho kigamije kumenya impamyabumenyi 15 PapilOmavirus yagira ingaruka ku bantu 15.

Ibisobanuro birambuye

Ibicuruzwa

Izina ry'ibicuruzwa

HWTS-CC005A-UBWOKO BW'INGINGO Z'INGENZI ZIKURIKIRA PAPILLOMAVIRUS YIMUNTU E6 / E7 Gene SE MRNA Kumenya (Fluorescence PCR)

Epidemiology

Kanseri y'inkondo y'umura ni bumwe mu bwoko bwa kanseri y'isi yose ku isi hose, kandi ibintu byayo bifitanye isano rya bugufi na Papillomavirsus y'abantu (HPV), ariko indwara nto gusa irashobora gukura muri kanseri. Hanze cyane HPV yanduye selituli yo mu maso kandi itanga oncoproteins ebyiri, E6 na E7. Iyi poroteyine irashobora kugira ingaruka kuri poroteyine zitandukanye (nk'ibibyimba byo guhagarika prb na p53), bigira ingaruka ku kanwa ka Dna no gutuza, no kubangamira ibikorwa bya antitive na antitumor.

Umuyoboro

Umuyoboro	Ibice	Genotoype yageragejwe
Umuryango	Hpv reaction buffer 1	HPV16,31,33,33,51,52,58
Vic / Hex	Hpv reaction buffer 1	Umuntu β-actin gene
Umuryango	Hpv reaction buffer 2	HPV 18.39,45,53,56.59,59,68
Vic / Hex	Hpv reaction buffer 2	Ins ins gene

Tekinike

Ububiko	Amazi: ≤-18 ℃
Imibereho-Ubuzima	Amezi 9
Ubwoko bw'ikigereranyo	Switil swab
Ct	≤38
CV	<5.0%
Lod	Kopi 500 / ML
Ibikoresho bisabwa	Gukoresha Biosystems 7500 Igihe cya PCRGushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Quartudio®5 sisitemu nyayo PCR Scan-96p sisitemu nyayo pcr sisitemu Umucyo®480 Igihe Cyigihe cya PCR Linegene 9600 wongeyeho sisitemu yigihe cya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR

Akazi

Basabwe gukuramo Reagent: Macro & Micro-Ikizamini cya virusi ya virusi (hwts-3020 -50-3020-HPV15) na Jial-Ikizamini Med-Tech Com-Tech Com-Tech Com . Umubumbe wabisabwa usabwe ni 50μl. Niba icyitegererezo kitacugirwa burundu, subiza ku ntambwe ya 4 yo gutangaza. Hanyuma hanyuma ugerageze ukurikije amabwiriza yo gukoresha.

Gusaba gukuramo reagent: Rnaprep Amatungo meza yinyamanswa Igikoresho cya RNA kinyuranyije na RNA (DP431). Gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha neza (mu ntambwe ya 5, inshuro ebyiri mnasei (1500u) igisubizo gishya cya RNASEI-KUBUNTU, Ongeramo 60μl ya RDD Buffer, hanyuma uvange witonze). Umubumbe wo hejuru wasabwe ni 60μl. Niba icyitegererezo kitacugirwa burundu, subiza ku ntambwe ya 5 yo gutangaza. Hanyuma hanyuma ugerageze ukurikije amabwiriza yo gukoresha.

Mbere: Umuntu wa Papillomavirus (ubwoko 28) genotoyping

Ibikurikira: VIH 1/2 Antibody

Andika ubutumwa bwawe hano hanyuma utwohereze