Ubwoko 15 bwa virusi ya papilloma ya muntu E6/E7 Gene mRNA ifite ibyago byinshi
Izina ry'igicuruzwa
Ubwoko bwa HWTS-CC005A-15 bwa Papillomavirusi y’abantu ifite ibyago byinshi byo kwandura Gene mRNA E6/E7 (Fluorescence PCR)
Ubumenyi bw'indwara
Kanseri y'inkondo y'umura ni imwe mu ndwara zikunze kugaragara ku bagore ku isi, kandi iyi kanseri ifitanye isano rya bugufi na virusi ziterwa na papillomavirusi (HPV), ariko igice gito cyane cy'ubwandu bwa HPV ni cyo gishobora kuvamo kanseri. HPV ifite ibyago byinshi yandura uturemangingo tw'inkondo y'umura ikabyara poroteyine ebyiri za oncoproteine, E6 na E7. Iyi poroteyine ishobora kugira ingaruka ku bwoko butandukanye bwa poroteyine (nk'iy'ibibyimba byongera kanseri pRB na p53), yongera igihe cy'uruhererekane rw'uturemangingo, igira ingaruka ku ikorwa rya ADN n'uburyo ihagaze, kandi ikabangamira uburyo ubudahangarwa bw'umubiri buterwa na virusi n'ubudahangarwa bw'umubiri burwanya kanseri.
Umuyoboro
| Umuyoboro | Igice | Genotype yapimwe |
| FAM | Umuti wa HPV Reaction Buffer 1 | HPV16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Ingirabuzimafatizo za β-actin z'umuntu | |
| FAM | Umuti wa HPV Reaction Buffer 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Ingirabuzimafatizo za INS z'umuntu |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18℃ |
| Igihe cyo kumara | Amezi 9 |
| Ubwoko bw'ingero | Igitambaro cyo mu mura |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Kopi 500/mL |
| Ibikoresho Bikoreshwa | Sisitemu ya PCR ikoreshwa mu gihe nyacyo ya 7500Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 Sisitemu za PCR za QuantStudio®5 mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo Sisitemu ya LightCycler®480 Real-Time PCR Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Uburyo bwo gukuramo ibintu busabwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Gukuramo ibintu bigomba gukorwa hakurikijwe amabwiriza yo kubikoresha. Ingano y’ibipimo byatanzwe ni 50μL. Niba icyitegererezo kidashongeshejwe neza, gisubize ku ntambwe ya 4 kugira ngo cyongere kigere ku gipimo. Hanyuma upime ukurikije amabwiriza yo kubikoresha.
Uburyo bwo gukuramo busabwa: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431). Gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha neza (Mu ntambwe ya 5, gwiza kabiri urugero rw'umuti ukoreshwa na DNaseI, ni ukuvuga gufata 20μL y'umuti ukoreshwa na RNase-Free DNaseI (1500U) mu muyoboro mushya wa RNase-Free centrifuge, ongeramo 60μL y'umuti ukoreshwa na RDD, hanyuma uvange buhoro buhoro). Ingano ya elution isabwa ni 60μL. Niba icyitegererezo kitaratunganywa neza, gisubize ku ntambwe ya 5 kugira ngo cyongere kinyobwa. Hanyuma ugerageze ukurikije amabwiriza yo gukoresha.
