Virusi ya Zaire
Izina RY'IGICURUZWA
HWTS-FE008 Zaire Ebola Virus Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Virusi ya Ebola ni iya Filoviridae, ikaba ari virusi ya RNA itagabanijwe.Virusi ni filime ndende ifite impuzandengo ya virusi ya 1000nm na diameter ya 100nm.Indwara ya virusi ya Ebola ni RNA itagabanijwe kandi ifite ubunini bwa 18.9kb, ikubiyemo poroteyine 7 zubaka na poroteyine 1 idafite imiterere.Virusi ya Ebola irashobora kugabanywamo ubwoko nka Zaire, Sudani, Bundibugyo, Ishyamba rya Tai na Reston.Muri bo, ubwoko bwa Zaire n'ubwoko bwa Sudani byavuzwe ko bitera abantu benshi bapfa bazize indwara.EHF (Ebola Hemorrhagic Fever) ni indwara ikaze yanduye hemorhagie iterwa na virusi ya Ebola.Abantu banduye cyane cyane guhura namazi yumubiri, gusohora no gusohora abarwayi cyangwa inyamaswa zanduye, kandi kwigaragaza kwa muganga usanga ahanini byerekana umuriro, kuva amaraso no kwangirika kwingingo nyinshi.EHF ifite impfu nyinshi zingana na 50% -90%.
Umuyoboro
| FAM | MP aside nucleic |
| ROX | Igenzura ryimbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 9 |
| Ubwoko bw'icyitegererezo | Serumu nshya 、 Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Amakopi 500 / μL |
| Umwihariko | Koresha ibikoresho kugirango ugerageze ibigo bibi, ibisubizo byujuje ibisabwa. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR SLAN-96P Sisitemu nyayo-PCR (Hongshi Medical Technology Co., Ltd.) UmucyoCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu Yigihe-PCR yo Kumenya (FQD-96A, Ikoranabuhanga rya Hangzhou Bioer) MA-6000 Igihe Cyuzuye Cyumubare Wumukino Wamagare (Suzhou Molarray Co., Ltd.) BioRad CFX96 Sisitemu Yukuri-Igihe PCR, na BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Ihitamo 1.
Gusabwa gukuramo reagent: QIAamp Viral RNA Mini Kit (52904), Gukuramo Acide Nucleic Acide cyangwa Isukura Reagent (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd.Igomba gukururwa hakurikijwe amabwiriza, kandi ingano yo gukuramo icyitegererezo ni 140μL naho icyifuzo cyo gukuraho ni 60μL.
Icya 2.
Gusabwa gukuramo reagent: Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acide Extract (HWTS-3006) .Ni bigomba gukururwa ukurikije amabwiriza.Ingano yo gukuramo urugero ni 200μL, naho icyifuzo cyo gukuraho ni 80μL.
Icya 3.
Gusabwa gukuramo reagent: Gukuramo Acide Nucleic Acide Reagent (1000020261) hamwe na sisitemu yo hejuru yo gutangiza ibyitegererezo byateguwe (MGISP-960) na BGI bigomba gukururwa ukurikije amabwiriza.Ingano yo gukuramo ni 160μL, naho icyifuzo cyo gukuraho ni 60μL.
