Virusi ya Ebola ya Zaire
Izina ry'igicuruzwa
HWTS-FE008 Zaire Virus Ebola Nucleic Acid Detection Kit (Fluorescence PCR)
Ubumenyi bw'indwara
Virusi ya Ebola ni iya Filoviridae, ikaba ari virusi ya RNA ifite umugozi umwe udafite ibice. Virusi ni uduce duto dufite uburebure bwa viriyoni 1000 n'umurambararo wa 100 nm. Genome ya virusi ya Ebola ni RNA ifite umugozi udafite ibice ifite ingano ya 18.9kb, ifite poroteyine 7 z'imiterere na poroteyine imwe idashingiye ku miterere. Virusi ya Ebola ishobora kugabanywamo ubwoko nka Zaire, Sudani, Bundibugyo, Tai Forest na Reston. Muri zo, ubwoko bwa Zaire n'ubwoko bwa Sudani byavuzwe ko bitera impfu nyinshi z'abantu bazize ubwandu. EHF (Ebola Hemorrhagic Fever) ni indwara yandura iterwa n'amaraso akabije iterwa na virusi ya Ebola. Abantu bandura ahanini iyo bahuye n'amazi, imyanda n'imyanda y'abarwayi cyangwa inyamaswa zanduye, kandi ibimenyetso by'indwara ni umuriro ugaragara, kuva amaraso no kwangirika kw'ingingo nyinshi. EHF ifite igipimo cyo hejuru cy'impfu kiri hagati ya 50% na 90%.
Umuyoboro
| FAM | aside nucleique ya MP |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 9 |
| Ubwoko bw'ingero | Serum nshya, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Kopi 500/μL |
| Ubwihariye | Koresha ibikoresho kugira ngo ugerageze ibimenyetso bibi by’ikigo, ibisubizo byujuje ibisabwa. |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 Sisitemu za PCR za QuantStudio®5 mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.) Sisitemu ya LightCycler®480 Real-Time PCR Sisitemu yo Gupima PCR ya LineGene 9600 Plus mu gihe nyacyo (FQD-96A, ikoranabuhanga rya Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermocycler (Suzhou Molarray Co., Ltd.) Sisitemu ya BioRad CFX96 Real-Time PCR, na Sisitemu ya BioRad CFX Opus 96 Real-Time PCR |
Urugendo rw'akazi
Amahitamo ya 1.
Reagent isabwa gukurwamo: QIAamp Viral RNA Mini Kit (52904), Reagent yo gukuramo aside ya Nucleic cyangwa Purification (YDP315-R) ya Tiangen Biotech (Beijing) Co., Ltd. Igomba gukurwamo hakurikijwe amabwiriza, kandi ingano y'icyitegererezo isabwa gukurwamo ni 140μL naho ingano y'icyitegererezo isabwa gukurwamo ni 60μL.
Amahitamo ya 2.
Reagent isabwa gukurwamo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Igomba gukurwamo hakurikijwe amabwiriza. Ingano y'icyitegererezo cyo gukurwamo ni 200μL, naho ingano y'icyitegererezo cyo gukurwamo ni 80μL.
Amahitamo ya 3.
Ibikoresho byo gukuramo ibinyabutabire byasabwe: Ibikoresho byo gukuramo aside ya nucleic (1000020261) na sisitemu yo gutegura icyitegererezo cya High Throughput Automated Sample (MGISP-960) na BGI bigomba gukurwamo hakurikijwe amabwiriza. Ingano yo gukuramo ni 160μL, naho ingano yo gukuramo ibinyabutabire yasabwe ni 60μL.
