Zaire Virusi
Izina ry'ibicuruzwa
Hwts-Fe008 Zaire Ebola Virus Nucleic Nuclection Kumenya (Fluorescence PCR)
Epidemiology
Virusi ya Ebola ni iya Filoviridae, ni virusi idahwitse-yandujwe na RNA. Virusi ni fireme ndende hamwe nuburebure bwa virusi ya 1000nm na diameter ya 100nm. Genous virusi ya Ebola nigitekerezo cyiza-strand cna ifite ubunini bwa 18.9kb, koden poroteyine 7 zububiko na poroteyine 1 idahwitse. Virusi ya Ebola irashobora kugabanywamo ubwoko nka Zaire, Sudani, Bundibugyo, ishyamba rya Tai na Reston. Muri bo, ubwoko bwa Zaire na Sudani byavuzwe ko bapfuye abantu benshi bandura. EHF (Ebola Hemorrhagic Forver) ni indwara yandura hemorrhagic yanduye yatewe na virusi ya Ebola. Abantu banduye cyane cyane bahura namazi yumubiri, ibanga no kuvuga abarwayi cyangwa excreta, inyamaswa zanduye zirimo umuriro, ibyangiritse byinshi. EHF ifite umubare wimfura ya 50% -90%.
Umuyoboro
| Umuryango | Acide ya mpcleic |
| Rox | Kugenzura imbere |
Tekinike
| Ububiko | ≤-18 ℃ |
| Imibereho-Ubuzima | Amezi 9 |
| Ubwoko bw'ikigereranyo | Urwenya rushya, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| Lod | Kopi 500 / μl |
| Umwihariko | Koresha ibikoresho byo kwipimisha ikizamini, ibisubizo byujuje ibisabwa. |
| Ibikoresho bisabwa | Gushyira mu bikorwa biosystems 7500 igihe cya sisitemu ya PCR Gushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Quartudio®5 sisitemu nyayo PCR Slan-96p Sisitemu NYAKURI PCR (Tekinoroji yubuvuzi Cology Co, Ltd.) Umucyo®480 Igihe Cyigihe cya PCR Umurongo 9600 wongeyeho sisitemu yigihe cya PCR (FQD-96a, Hangzhou tekinoroji yibinyabuzima) Ma-6000 nyayo-nyayo Umwanya wa Cyrmal Cycler (Suzhou Morlary Co, Ltd.) Biorad CFX96 Igihe cya PCR nyayo, na Biorad CFX Opes 96 Igihe cya PCR |
Akazi
Ihitamo rya 1.
Gusaba gukuramo reagent: Qiaip viral rna mini kit (52904), acide ya nucleic cyangwa isuku Igomba gukurwa mu buryo bukomeye ukurikije amabwiriza, kandi umubare wasabwe w'icyitegererezo ni 140μl hamwe n'ijwi risabwa na 60μl.
IHitamo 2.
Gusaba Gukuramo Reagent: Macro-Ikizamini cya Viral ADN / RNA (HWTS-3004-32, HWTS-3004-96) na micro bigomba gukurwa ukurikije amabwiriza. Gukuramo icyitegererezo ni 200μl, hamwe nububiko bwabigenewe ni 80μl.
Ihitamo rya 3.
Basabwe gusubiramo reagenction: Gukuramo acide nucleic Igitabo cyo gukuramo ni 160μl, hamwe nububiko bwabigenewe ni 60μl.
