SARS-CoV-2 / ibicurane A / ibicurane B.

Ibisobanuro bigufi:

Iki gikoresho gikwiranye na vitro yujuje ubuziranenge bwa SARS-CoV-2, ibicurane A na grippe B nucleic acide ya nasopharyngeal swab na oropharyngeal swab icyitegererezo mu bantu bakekwagaho kwandura SARS-CoV-2, ibicurane A na grippe. B. Irashobora kandi gukoreshwa mugukekwaho umusonga no gukekwaho indwara ya cluster no gutahura ubuziranenge no kumenya SARS-CoV-2, ibicurane A na grippe B nucleic acide muri nasopharyngeal swab na oropharyngeal swab ingero zanduye Coronavirus mubindi bihe.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-RT148-SARS-CoV-2 / ibicurane A / ibicurane B Nucleic Acide Ikomatanyirijwe hamwe (Fluorescence PCR)

Umuyoboro

Izina ry'umuyoboro	PCR-Kuvanga 1	PCR-Kuvanga 2
Umuyoboro wa FAM	ORF1ab	IVA
Umuyoboro wa VIC / HEX	Kugenzura imbere	Kugenzura imbere
CY5 Umuyoboro	N gen	/
Umuyoboro ROX	E gen	IVB

Ibipimo bya tekiniki

Ububiko	-18 ℃
Ubuzima bwa Shelf	Amezi 12
Ubwoko bw'icyitegererezo	nasopharyngeal swabs na oropharyngeal swabs
Intego	SARS-CoV-2 intego eshatu (Orf1ab, N na E genes) / ibicurane A / ibicurane B
Ct	≤38
CV	≤10.0%
LoD	SARS-CoV-2 ： 300 Amakopi / mL ibicurane A virusi ： 500 Kopi / mL virusi ya grippe B Cop 500 Kopi / mL
Umwihariko	a) Ibisubizo by'ibizamini byambukiranya imipaka byerekanaga ko ibikoresho byari bihuye na coronavirus ya muntu SARSr- CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, virusi y'ubuhumekero A na B, virusi ya parainfluenza 1, 2 na 3, rhinovirusA, B na C, adenovirus 1, 2, 3, 4, 5, 7 na 55, metapneumovirus yumuntu, enterovirus A, B, C na D, virusi ya cytoplasmeque yumuntu, virusi ya EB, virusi yindwara ya cytomegalovirus, rotavirus, norovirus, virusi ya mumps, virusi ya varicella zoster, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, ibicurane bya Haemophilus, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klele twe, Candida albicans, Candida glabrata Nta reaction ya cross hagati ya Pneumocystis yersini na Cryptococcus neoformans. b) Ubushobozi bwo kurwanya kwivanga: hitamo mucin (60mg / mL), 10% (V / V) maraso yumuntu, diphenylephrine (2mg / mL), hydroxymethylzoline (2mg / mL), chloride ya sodiumi (irimo imiti igabanya ubukana) (20mg / mL), beclomethasone (20mg / mL), dexamethasone (20mg / mL), flunisone (20μg / mL), acetonide ya triamcinolone (2mg / mL), budesonide (2mg / mL), mometasone (2mg / mL), fluticasone (2mg / mL), hydamine hydrochloride (5mg / mL), α-Interferon (800IU / mL), zanamivir (20mg / mL), ribavirin (10mg / mL), oseltamivir (60ng / mL), pramivir (1mg / mL), lopinavir (500mg / mL) ), ritonavir (60mg / mL), mupirocine (20mg / mL), azithromycine (1mg / mL), ceprotene (40μg / mL) Meropenem (200mg / mL), levofloxacin (10μg / mL) na tobramycine (0,6mg / mL) .Ibisubizo byerekanye ko ibintu bivangavanze hejuru yibitekerezo byavuzwe haruguru nta gisubizo kibangamiye ibisubizo byatewe na virusi.
Ibikoresho bikoreshwa	Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR SLAN^®-96P Sisitemu-Igihe nyacyo PCR QuantStudio ™ 5 Sisitemu-Igihe nyacyo PCR Umucyo^®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR