SARS-CoV-2 / ibicurane A / ibicurane B.

Ibisobanuro bigufi:

Iki gikoresho gikwiranye na vitro yujuje ubuziranenge bwa SARS-CoV-2, ibicurane A na grippe B nucleic acide ya nasopharyngeal swab na oropharyngeal swab urugero mu bantu bakekwagaho kwandura SARS-CoV-2, ibicurane A na grippe B. Birashobora kandi gukoreshwa mu bantu bakekwaho kwandura indwara ya grippe A na virusi. ibicurane B nucleic acide muri nasopharyngeal swab na oropharyngeal swab ingero zanduye ya Coronavirus yanduye mubindi bihe.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Izina ryibicuruzwa

HWTS-RT148-SARS-CoV-2 / ibicurane A / ibicurane B Nucleic Acide Ikomatanyirijwe hamwe (Fluorescence PCR)

Umuyoboro

Izina ry'umuyoboro	PCR-Kuvanga 1	PCR-Kuvanga 2
Umuyoboro wa FAM	ORF1ab	IVA
Umuyoboro wa VIC / HEX	Kugenzura imbere	Kugenzura imbere
CY5 Umuyoboro	N gen	/
Umuyoboro ROX	E gen	IVB

Ibipimo bya tekiniki

Ububiko	-18 ℃
Ubuzima bwa Shelf	Amezi 12
Ubwoko bw'icyitegererezo	nasopharyngeal swabs na oropharyngeal swabs
Intego	SARS-CoV-2 intego eshatu (Orf1ab, N na E genes) / ibicurane A / ibicurane B
Ct	≤38
CV	≤10.0%
LoD	SARS-CoV-2 ： 300 Amakopi / mL ibicurane A virusi ： 500 Kopi / mL virusi ya grippe B Cop 500 Kopi / mL
Umwihariko	a) Ibisubizo by'ibizamini byambukiranya imipaka byerekanaga ko ibikoresho byari bihuye na coronavirus ya muntu SARSr- CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, virusi ya syncytial A na B, virusi ya parainfluenza 1, 2 na 3, rhinovirusA, 2 7 na 55, metapneumovirus yumuntu, enterovirus A, B, C na D, virusi ya cytoplasmic pulmonary virusi, virusi ya EB, virusi yiseru Umuntu cytomegalovirus, rotavirus, norovirus, virusi ya virusi, varicella zoster virusi, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertusus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculose, Aspergillus fumigatus, Candida albicans, Candida glabrata Nta reaction yambutse hagati ya Pneumocystis yersini na Cryptococcus neoformans. b) Ubushobozi bwo kurwanya kwivanga: hitamo mucin (60mg / mL), 10% (V / V) maraso yumuntu, diphenylephrine (2mg / mL), hydroxymethylzoline (2mg / mL), sodium chloride (irimo preservateur) (20mg / mL), beclomethasone (20mg / mL), dexamethasone (20mg / mL), acetonide (2mg / mL), budesonide (2mg / mL), mometasone (2mg / mL), fluticasone (2mg / mL), hydrochloride ya histamine (5mg / mL), α-Interferon (800IU / mL), zanamivir (20mg / mL), ribavirin (10mg / mL), lopinavir (500mg / mL), ritonavir (60mg / mL), mupirocine (20mg / mL), azithromycine (1mg / mL), ceprotene (40μg / mL) Meropenem (200mg / mL), levofloxacin (10μg / mL) na tobramycine (0,6mg / mL). Ibisubizo byerekanye ko ibintu bivangavanze hejuru yibitekerezo byavuzwe haruguru nta gisubizo kibangamiye ibisubizo byatewe na virusi.
Ibikoresho bikoreshwa	Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR SLAN^®-96P Sisitemu-Igihe nyacyo PCR QuantStudio ™ 5 Sisitemu-Igihe nyacyo PCR Umucyo^®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR