Ubwoko butandatu bw'indwara zitera indwara zo mu myanya y'ubuhumekero

Ibisobanuro bigufi:

Iyi mashini ishobora gukoreshwa mu gupima neza aside nucleic ya SARS-CoV-2, virusi ya grippe A, virusi ya grippe B, adenovirus, mycoplasma pneumoniae na virusi ya respiratory syncytial virus muri vitro.

Ohereza imeri kuri twe

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Izina ry'igicuruzwa

HWTS-OT058A/B/C/Z-Kit ya RT-PCR ikoresha fluorescent y'igihe nyacyo ikoreshwa mu gupima ubwoko butandatu bw'udukoko two mu myanya y'ubuhumekero

Icyemezo

Ubumenyi bw'indwara

Indwara ya Virusi ya Korona 2019, yitwa "COVID-19", yerekeza ku musonga uterwa n'ubwandu bwa SARS-CoV-2. SARS-CoV-2 ni coronavirus iri mu bwoko bwa β. COVID-19 ni indwara yandura ifata imyanya ndangagitsina y'ubuhumekero, kandi abaturage muri rusange ni bo bashobora kwibasirwa. Muri iki gihe, inkomoko y'ubwandu ahanini ni abarwayi banduye SARS-CoV-2, kandi abantu banduye badafite ibimenyetso bashobora no kuba intandaro y'ubwandu. Hashingiwe ku bushakashatsi bwakozwe ubu ku ndwara, igihe cyo kwibasira ni iminsi 1-14, cyane cyane iminsi 3-7. Umuriro, inkorora yumye n'umunaniro ni byo bimenyetso by'ingenzi. Abarwayi bake bari bafite izuru rizinze, amazuru asohoka, mu muhogo ubabara, mu mihogo no mu mpiswi.

Influenza, izwi cyane nka "grippe", ni indwara y'ubuhumekero iterwa na virusi ya grippe. Irandura cyane. Yandurira ahanini mu gukorora no kwitsamura. Akenshi ivuka mu mpeshyi no mu gihe cy'itumba. Virusi za grippe zigabanyijemo ibice bibiri: grippe A, IFV A, grippe B, IFV B, na grippe C, IFV C, byose biri mu bwoko bwa virusi ifatana, itera indwara ku bantu cyane cyane virusi za grippe A na B, ni virusi ya RNA ifite umurongo umwe, igabanyijemo ibice. Virusi ya grippe A ni indwara y'ubuhumekero ifatana, irimo H1N1, H3N2 n'izindi, zishobora guhinduka no kugaragara ku isi yose. "Shift" yerekeza ku guhinduka kwa virusi ya grippe A, bigatuma habaho "ubwoko bumwe" bwa virusi. Virusi za grippe B zigabanyijemo ibice bibiri, Yamagata na Victoria. Virusi ya grippe B ifite gusa antigenic drift, kandi yirinda kugenzura no gukuraho kw'ubudahangarwa bw'umubiri bw'umuntu binyuze mu guhinduka kwayo. Ariko, umuvuduko w'impinduka za virusi ya grippe B ugenda buhoro ugereranije n'uwa grippe A ku bantu. Virusi ya Grippe B ishobora kandi gutera indwara z'ubuhumekero ku bantu ndetse igatera n'ibyorezo by'indwara.

Adenovirus (AdV) ikomoka kuri adenovirus y’inyamabere, ikaba ari virusi ya ADN ifite ibice bibiri idafite aho iboheye. Nibura ubwoko 90 bw’uturemangingo twabonetse, dushobora kugabanywamo ubwoko butatu bwa AG 7. Ubwandu bwa AdV bushobora gutera indwara zitandukanye, harimo umusonga, bronchitis, cystite, conjunctivitis y’amaso, indwara zo mu gifu na encephalitis. Adenovirus pneumonia ni imwe mu ndwara z’umusonga ziterwa n’abaturage mu bana, ikaba ingana na 4%-10% by’umusonga uterwa n’abaturage mu baturage.

Mycoplasma pneumoniae (MP) ni ubwoko bw'uturemangingo duto cyane twa prokaryotic, turi hagati ya bagiteri na virusi, dufite imiterere y'uturemangingo ariko nta rukuta rw'uturemangingo. MP ahanini itera indwara z'imiyoboro y'ubuhumekero ku bantu, cyane cyane ku bana n'urubyiruko. Ishobora gutera indwara ya mycoplasma mu bantu, indwara z'imiyoboro y'ubuhumekero ku bana ndetse n'indwara ya pneumonia idasanzwe. Ibimenyetso by'indwara biratandukanye, inyinshi muri zo ni inkorora ikomeye, umuriro, ubukonje, kuribwa umutwe, kubabara mu muhogo. Indwara z'imiyoboro y'ubuhumekero yo hejuru n'indwara ya pneumonia yo mu muhogo ni byo bikunze kugaragara cyane. Hari abarwayi bashobora kuva ku ndwara z'imiyoboro y'ubuhumekero yo hejuru bakagera ku ndwara ya pneumonia ikomeye, kubabara cyane mu myanya y'ubuhumekero ndetse n'urupfu rushobora kubaho.

Virusi ya Respiratory syncytial virus (RSV) ni virusi ya RNA, ikomoka mu muryango wa paramyxoviridae. Yandurira mu bitonyanga by'umwuka no mu buryo bwa hafi, ikaba ari yo ndwara nyamukuru itera ubwandu bw'imitsi yo mu myanya y'ubuhumekero mu bana bato. Abana banduye RSV bashobora kurwara bronchiolitis ikomeye (izwi nka bronchiolitis) na pneumonia, bifitanye isano na asima mu bana. Abana bato bafite ibimenyetso bikomeye, birimo umuriro mwinshi, rhinitis, pharyngitis na laryngitis, hanyuma bronchiolitis na pneumonia. Abana bake barwaye bashobora kugorwa n'indwara ya otitis media, pleurisy na myocarditis, nibindi. Ubwandu bw'imitsi yo mu myanya y'ubuhumekero yo hejuru ni cyo kimenyetso cy'ubwandu ku bantu bakuru n'abana bakuru.

Umuyoboro

Izina ry'umuyoboro	R6 Reaction Buffer A	R6 Reaction Buffer B
FAM	SARS-CoV-2	HAdV
VIC/HEX	Igenzura ry'imbere	Igenzura ry'imbere
CY5	IFV A	MP
ROX	IFV B	Indwara ya RSV

Ibipimo bya tekiniki

Ububiko	Amazi: ≤-18℃ Mu mwijima; Ifite impumuro mbi: ≤30℃ Mu mwijima
Igihe cyo kumara	Amazi: Amezi 9; Amavuta ya Lyophilized: Amezi 12
Ubwoko bw'ingero	Amaraso yose, Plasma, Serum
Ct	≤38
CV	≤5.0％
LoD	Kopi 300/mL
Ubwihariye	Ibisubizo byatanzwe byagaragaje ko nta kibazo cyabayeho hagati y’ibikoresho byafashwe n’indwara ya coronavirus y’abantu SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, virusi ya parainfluenza ubwoko bwa 1, 2, 3, virusi ya rhinovirus A, B, C, chlamydia pneumoniae, virusi ya metapneumovirus y’abantu, enterovirus A, B, C, D, virusi y’ibihaha by’abantu, virusi ya epstein-barr, virusi ya measles, virusi ya cytomegalo y’abantu, virusi ya rotavirus, virusi ya norovirus, virusi ya parotitis, virusi ya varicella-zoster, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s. pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci na newborn cryptococcus na human genomic nucleic acid.
Ibikoresho Bikoreshwa	Ishobora guhuza n'ibikoresho bya PCR bikoreshwa mu gupima urumuri biri ku isokoSisitemu za PCR za SLAN-96P mu gihe nyacyo Sisitemu za PCR za ABI 7500 mu gihe nyacyo Sisitemu za PCR za ABI 7500 zihuta cyane mu gihe nyacyo QuantStudio^®Sisitemu 5 za PCR mu gihe nyacyo LightCycler^®Sisitemu 480 za PCR mu gihe nyacyo Sisitemu zo Gusuzuma za LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR y'igihe nyacyo, BioRad Sisitemu ya PCR ya CFX Opus 96 mu gihe nyacyo