Ubwoko butandatu bwindwara zubuhumekero
Izina RY'IGICURUZWA
HWTS-OT058A / B / C / Z-Igihe nyacyo fluorescent RT-PCR ibikoresho byo kumenya ubwoko butandatu bwindwara zubuhumekero
Icyemezo
CE
Epidemiologiya
Indwara ya Corona Virusi 2019, yitwa "COVID-19", yerekeza ku musonga watewe n'indwara ya SARS-CoV-2.SARS-CoV-2 ni coronavirus yo mu bwoko bwa β.COVID-19 ni indwara yandurira mu myanya y'ubuhumekero, kandi muri rusange abaturage bakunze kwibasirwa.Kugeza ubu, isoko yandura ni abarwayi banduye SARS-CoV-2, kandi abanduye simptomatique nabo bashobora kuba intandaro yo kwandura.Ukurikije iperereza ryakozwe muri iki gihe, igihe cyo gukuramo ni iminsi 1-14, cyane cyane iminsi 3-7.Umuriro, inkorora yumye n'umunaniro nibyo bigaragara.Abarwayi bake barwaye izuru, izuru ritemba, kubabara mu muhogo, myalgia na diyare.
Ibicurane, bizwi cyane ku izina rya "ibicurane", ni indwara ikabije y’ubuhumekero iterwa na virusi ya grippe.Yanduye cyane.Yandura cyane cyane gukorora no kwitsamura.Ubusanzwe itangira mu mpeshyi no mu itumba.Virusi y'ibicurane igabanyijemo ibicurane A, IFV A, ibicurane B, IFV B, na Grippe C, IFV C ubwoko butatu, byose ni virusi ifatanye, bitera indwara zabantu cyane cyane virusi ya grippe A na B, ni umurongo umwe, virusi ya RNA.Indwara y'ibicurane A ni indwara ikabije y'ubuhumekero, harimo H1N1, H3N2 hamwe n'ubundi bwoko, bukunze kwibasirwa n'imihindagurikire y'ikirere."Shift" bivuga ihinduka rya virusi ya grippe A, bigatuma havuka virusi nshya "subtype".Virusi y'ibicurane B igabanijwemo imirongo ibiri, Yamagata na Victoria.Virusi ya grippe B ifite gusa antigenic drift, kandi irinda igenzura ryumubiri w’umuntu no kurandurwa binyuze mu ihinduka ry’imihindagurikire.Nyamara, umuvuduko w’ihindagurika rya virusi ya grippe B uratinda cyane ugereranije na virusi ya grippe A.Umugera wa grippe B urashobora kandi gutera indwara zubuhumekero zabantu kandi biganisha ku byorezo.
Adenovirus (AdV) ni iy'inyamabere adenovirus y’inyamabere, ikaba ari virusi ya ADN ikubye kabiri idafite ibahasha.Nibura genotypes 90 zabonetse, zishobora kugabanywa muri AG 7 subgenera.Indwara ya AdV irashobora gutera indwara zitandukanye, zirimo umusonga, bronhite, cystite, conjunctivitis y'amaso, indwara zo mu gifu na encephalite.Umusonga wa Adenovirus ni bumwe mu bwoko bukabije bw’umusonga wanduye mu bana, bangana na 4% -10% by'umusonga wanduye abaturage.
Mycoplasma pneumoniae (MP) ni ubwoko bwa mikorobe ntoya ya prokaryotic, iri hagati ya bagiteri na virusi, ifite imiterere y'utugari ariko nta rukuta rw'akagari.Depite ahanini itera indwara zubuhumekero zabantu, cyane cyane kubana ndetse nabasore.Irashobora gutera mycoplasma pneumonia yumuntu, kwandura kwubuhumekero bwabana hamwe numusonga udasanzwe.Kugaragara kwa clinique biratandukanye, ibyinshi muri byo ni inkorora ikabije, umuriro, gukonja, kubabara umutwe, kubabara mu muhogo.Indwara y'ubuhumekero yo hejuru hamwe n'umusonga wa bronchial niwo ukunze kugaragara.Bamwe mu barwayi barashobora kwandura indwara zandurira mu myanya y'ubuhumekero kugeza umusonga ukabije, ibibazo by'ubuhumekero bikabije ndetse n'urupfu.
Indwara y'ubuhumekero (RSV) ni virusi ya RNA, ikomoka mu muryango wa paramyxoviridae.Yandura mu bitonyanga byo mu kirere no guhura cyane kandi ni yo ntandaro nyamukuru yandurira mu myanya y'ubuhumekero yo hasi.Impinja zanduye RSV zirashobora kurwara bronchiolitis zikomeye (bita bronchiolitis) na pnewoniya, zifitanye isano na asima mu bana.Impinja zifite ibimenyetso bikomeye, birimo umuriro mwinshi, rhinite, pharyngitis na laryngitis, hanyuma bronchiolitis na pnewoniya.Abana bake barwaye barashobora kugorana nibitangazamakuru bya otitis, pleurisy na myocarditis, nibindi. Indwara zo mu myanya y'ubuhumekero zo hejuru nicyo kimenyetso nyamukuru cyanduza abantu bakuru ndetse nabana bakuru.
Umuyoboro
Izina ry'umuyoboro | R6 Buffer Igisubizo A. | R6 Igisubizo Buffer B. |
FAM | SARS-CoV-2 | HAdV |
VIC / HEX | Igenzura ryimbere | Igenzura ryimbere |
CY5 | IFV A. | MP |
ROX | IFV B. | RSV |
Ibipimo bya tekiniki
Ububiko | Amazi: ≤-18 ℃ Mu mwijima;Lyophilized: ≤30 ℃ Mu mwijima |
Ubuzima bwa Shelf | Amazi: amezi 9;Lyophilized: amezi 12 |
Ubwoko bw'icyitegererezo | Amaraso yose, Plasma, Serumu |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 300Kopi / mL |
Umwihariko | Ibisubizo byambukiranya imipaka byerekanaga ko nta reaction yakozwe hagati yigitabo na coronavirus yumuntu SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, virusi ya parainfluenza ubwoko bwa 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus yumuntu, enterovirus A, B, C, D, virusi y’ibihaha y’abantu, virusi ya epstein-barr, virusi y’iseru, virusi ya cytomegalo, rotavirus, norovirus, virusi ya parotite, virusi ya varicella-zoster, legionella, bordetella pertussis, ibicurane bya haemophilus, staphylococcus aureus, streptococcus pneumoniae, s.pyogène, klebsiella pneumoniae, mycobacterium igituntu, umwotsi aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci hamwe na cryptococcus yavutse hamwe na acide genomic nucleic. |
Ibikoresho bikoreshwa | Irashobora guhuza ibikoresho nyamukuru bya fluorescent PCR kumasokoSLAN-96P Sisitemu nyayo-PCR ABI 7500 Sisitemu nyayo-PCR ABI 7500 Byihuse-Igihe Cyuzuye PCR QuantStudio®5 Sisitemu nyayo-PCR Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho-Igihe-Cyukuri cya PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu nyayo-PCR Sisitemu, BioRad CFX Opus 96 Sisitemu nyayo-PCR |