Indwara z'ubuhumekero zahujwe
Izina ryibicuruzwa
HWTS-RT183-Indwara y'ubuhumekero Ikomatanyirijwe hamwe (Fluorescence PCR)
Epidemiologiya
Indwara ya Corona Virusi 2019, yitwa 'COVID-19', yerekeza ku musonga watewe n'indwara ya SARS-CoV-2. SARS-CoV-2 ni coronavirus yo mu bwoko bwa β. COVID-19 ni indwara yandurira mu myanya y'ubuhumekero, kandi muri rusange abaturage bakunze kwibasirwa. Kugeza ubu, isoko yanduye ahanini ni abarwayi banduye 2019-nCoV, kandi abanduye simptomatic nabo bashobora kuba intandaro yo kwandura. Ukurikije iperereza ryakozwe muri iki gihe, igihe cyo gukora ni iminsi 1-14, cyane cyane iminsi 3-7. Umuriro, inkorora yumye n'umunaniro nibyo bigaragara. Abarwayi bake bari bafite ibimenyetso nko kuzunguruka mu mazuru, izuru ritemba, kubabara mu muhogo, myalgia na diyare, n'ibindi. Yanduye cyane. Yandura cyane cyane gukorora no kwitsamura. Ubusanzwe itangira mu mpeshyi no mu itumba. Virusi y'ibicurane igabanyijemo ibicurane A (IFV A), ibicurane B (IFV B), na Grippe C (IFV C) ubwoko butatu, byose ni virusi ifatanye, bitera indwara zabantu cyane cyane virusi ya grippe A na B, ni virusi ya RNA imwe. Indwara y'ibicurane A ni indwara ikabije y'ubuhumekero, harimo H1N1, H3N2 ndetse n'ubundi bwoko butandukanye, bukunze kwibasirwa n'imihindagurikire y'ikirere. 'Shift' bivuga ihinduka rya virusi ya grippe A, bigatuma habaho virusi nshya 'subtype'. Virusi ya grippe B igabanyijemo ibice bibiri, virusi ya Yamagata na Victoria ibicurane B ifite gusa antigenic drift, kandi irinda igenzura ry’umubiri w’umuntu no kurandura binyuze mu ihinduka ry’imihindagurikire. Nyamara, umuvuduko w’ihindagurika rya virusi ya grippe B uratinda cyane ugereranije na virusi ya grippe A. Virusi ya grippe B irashobora kandi gutera indwara zubuhumekero zabantu kandi biganisha ku byorezo.
Indwara y'ubuhumekero (RSV) ni virusi ya RNA, ikomoka mu muryango wa paramyxoviridae. Yandura mu bitonyanga byo mu kirere no guhura cyane kandi ni yo ntandaro nyamukuru yandurira mu myanya y'ubuhumekero yo hasi. Impinja zanduye RSV zirashobora kurwara bronchiolitis zikomeye n'umusonga, zifitanye isano na asima ku bana. Impinja zifite ibimenyetso bikomeye, birimo umuriro mwinshi, rhinite, pharyngitis na laryngitis, hanyuma bronchiolitis na pnewoniya. Abana bake barwaye barashobora kugorana nibitangazamakuru bya otitis, pleurisy na myocarditis, nibindi. Indwara yubuhumekero yo hejuru ni ikimenyetso nyamukuru cyanduza abantu bakuru ndetse nabana bakuru.
Ibipimo bya tekiniki
| Ububiko | -18 ℃ Mu mwijima |
| Ubuzima bwa Shelf | Amezi 9 |
| Ubwoko bw'icyitegererezo | Oropharyngeal swab; Nasopharyngeal swab |
| Ct | IFV A, IFVB, RSV, SARS-CoV-2, IFV A H1N1Ct≤38 |
| CV | ≤5% |
| LoD | Amakopi 200 / μL |
| Umwihariko | Ibisubizo bya cross-reactivite byerekana ko nta reaction yambukiranya hagati ya kit na cytomegalovirus, herpes simplex virusi yo mu bwoko bwa 1, virusi ya varicella zoster, virusi ya Epstein-Barr, adenovirus, metapneumovirus yumuntu, rhinovirus, virusi ya parainfluenza I / II / III / IV, bocavirus, enterovirus, coronavirusi Bordetella pertussis, Corynebacterium spp., Escherichia coli, ibicurane bya Haemophilus, Lactobacillus spp. aureus, Streptococcus pneumoniae, epidermidis ya Staphylococcus, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium fasciatum, Nocardiaccum Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis carinii, Candida albicans, Roseburia mucosa, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia Q umuriro hamwe na acide genomic nucleic. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe-PCR Sisitemu, Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-PCR Sisitemu, QuantStudio®Sisitemu 5-Igihe nyacyo PCR, SLAN-96P Sisitemu nyayo-PCR (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Sisitemu nyayo-PCR, LineGene 9600 Yongeyeho Sisitemu Yigihe Cyigihe cyo Kumenya PCR (FQD-96A, Ikoranabuhanga rya Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Sisitemu Yigihe-PCR, BioRad CFX Opus 96 Sisitemu Yigihe-PCR. |
Urujya n'uruza rw'akazi
Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3017) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B)), na Macro & Micro-Test Virus ADN / RNA Kit (HWTS-3017-8) (ishobora gukoreshwa na Eudemon.TM AIO800 (HWTS-EQ007)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ingano yicyitegererezo yakuweho ni 200μL naho ingano yo gusabwa ni 150μL.
