Orientia tsutsugamushi
Izina ry'igicuruzwa
HWTS-OT002B Orientia tsutsugamushiAgakoresho ko Gupima Aside Nucleic (Fluorescence PCR)
Ubumenyi bw'indwara
Indwara ya Scrub typhus ni indwara ikomeye iterwa n'ubwandu bwa Orientia tsutsugamushi (Ot). Orientia scrub typhus ni mikorobe itera indwara zo mu bwoko bwa Gram-negative. Orientia scrub typhus iri mu bwoko bwa Orientia mu cyiciro cya Rickettsiales, umuryango wa Rickettsiaceae, n'ubwoko bwa Orientia. Indwara ya Scrub typhus yandura ahanini binyuze mu kurumwa n'udukoko twa chigger dutwara indwara. Mu buvuzi irangwa no kugira umuriro mwinshi utunguranye, eschar, lymphadenopathy, hepatosplenomegaly, na leukopenia y'amaraso yo mu miyoboro y'amaraso, n'ibindi. Mu bihe bikomeye, ishobora gutera meningitis, umwijima n'impyiko kunanirwa, kunanirwa kw'ingingo nyinshi, ndetse no gupfa.
Umuyoboro
| FAM | Orientia tsutsugamushi |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | serumu nshya |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Kopi 500/μL |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.) LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu zo Gupima za LineGene 9600 Plus mu gihe nyacyo (FQD-96A, ikoranabuhanga rya Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermocycler (Suzhou Molarray Co., Ltd.) Sisitemu ya BioRad CFX96 ya PCR y'igihe nyacyo, Sisitemu ya BioRad CFX Opus 96 ya PCR y'igihe nyacyo |
Urugendo rw'akazi
Reagent isabwa gukurwamo: Macro na Micro-TestRusangeKit ya ADN/RNA (HWTS-301)9) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha iyi reagent yo gukuramo. Ingano y'icyitegererezo cyakuwemo ni 200µL, kandi ingano ya elution isabwa ni100µL.
