Intwaro nshya yo gusuzuma igituntu no Kumenyekanisha ibiyobyabwenge: Igisekuru cyo kurwanya ibiyobyabwenge: Igisekuru gishya cyagenewe gukurikiranwa na mashini yo kwiga igituntu hypersensitivites
Raporo yubuvanganzo: CCA: Icyitegererezo cyo gusuzuma kishingiye kuri Tngs na Wimashini bikaba bikwiranye nabantu bafite igituntu gito cya bagiteri na meningite meberculous.
Umutwe wa TheSsis: Igituba gikurikira-gisekuru gikurikiranwa na gisekuru gikurikira: Ingamba zo Gusuzuma Ultra-yoroshye ya Ultra-yoroshye ya Paucime ya Paucime
Ibihe: "Clinica Chimica Acta"
Niba: 6.5
Itariki yo gutangaza: Mutarama 2024
Ihujwe na kaminuza yishuri ryubumenyi na Beijing Ibitaro byubucuruzi bwa kaminuza nkuru yubuvuzi, Macro & Micro-Ikizamini cyashyizweho na tebercios ashingiye ku gishushanyo gikurikira (Tngs) Ikoranabuhanga hamwe na Ultra-Hejuru Gutangiza sensitivite kubitumbe hamwe na bagiteri nkeya na баngite meningitis, yatanze uburyo bushya bwo gusuzuma uburyohe bwo gusuzuma Isuzuma rya Clinical ryubwoko bubiri bwigituntu, kandi ryafashaga kwisuzumisha neza, gutahura ibiyobyabwenge no kuvura igituntu. Muri icyo gihe, harasanga plasma wumurwayi CFDNA ishobora gukoreshwa nkubwoko bwicyitegererezo cyo kwipimisha ubuvuzi mu gusuzuma tbm.
Muri ubu bushakashatsi, imvugo 227 za plasma hamwe ningero zubumara ryakoreshejwe mugushiraho icyitegererezo cya clicique yiga imashini yo gusuzuma ibitumbe, kandi ingufu za clicique yakoreshejwe mugusuzuma ibyashyizweho Moderi. Ibyitegererezo byose byabanje kwibasirwa numwanya wateguwe cyane wagenewe Probe Paol ya MycobaCerium. Noneho, ukurikije amakuru ya TB-Tngs, icyitegererezo cyitwa Restique gikoreshwa mugukora ibyemezo 5 byambukiranya amahugurwa no kwemeza amahugurwa yo gusuzuma umurongo, hamwe nimiti yo gusuzuma ya laboratoire ya laboratoire na plasma. Umubare wabonetse uzanwa mu maseti abiri yo gusuzuma umurongo wa clinical wo gutahura, kandi imikorere yo gusuzuma isuzumwa na ROC umurongo. Hanyuma, kwisuzumisha icyitegererezo cyibituntu byabonetse.
Igishushanyo 1 Igishushanyo cya Schematic Igishushanyo cyubushakashatsi
Ibisubizo: Ukurikije imbibi zihariye za CSF DNA (auc = 0.974) na plasma cfdna yicyitegererezo (auc = 0,908) yiteguye icyitegererezo cya CSF yari 97,65%, kandi Imitini n'umwihariko w'icyitegererezo cya Plasma cyari 82.61% na 86.36%. Mu isesengura ry'imitwe 44 yahujwe na Plasma CFDNA na Creabrospinal DNN kuva abarwayi ba TBM, ingamba zo gusuzuma kugeza kuri 90.91% (40/44) (42/44). Mubana bafite igituntu cya pubmoni, ingamba zo gusuzuma zirushijeho kumva imbaraga za plasma kuruta ibisubizo byumutobe wa gastric.
Igishushanyo cya 2 Gukora isesengura ry'igikoresho cyo kwisuzumisha igituntu ku byitegererezo by'abaturage
Igishushanyo 3 Ibisubizo by'icyitegererezo
UMWANZURO: Uburyo bwo gusuzuma uburyo bwo gusuzuma igituntu bwashyizweho muri ubu bushakashatsi, bushobora gutanga igikoresho cyo gusuzuma hamwe no kwiyumvisha abarwayi bavuza amashanyarazi hamwe na ogterculose (umuco mubi). Detection of hypersensitive tuberculosis based on plasma cfDNA may be a suitable sample type for the diagnosis of active tuberculosis and tuberculous meningitis (plasma samples are easier to collect than cerebrospinal fluid for patients suspected of brain tuberculosis).
Ihuza ryumwimerere: https://www.ciensiondirect.com/sions/article/pii/pii/s0009898123004990? Binyuze% 3diHub
Kumenyekanisha muri make Macro & Micro-Ikizamini cyo gupima igituntu cyo kumenya ibicuruzwa
Urebye ikibazo kitoroshye cyabarwayi ba Tuberculose nibikenewe bitandukanye, Macro-micro-micro-micro-micro-micro-micro-micro bitanga ibisubizo byuzuye byo gukuramo imitekerereze ya sputomu Ibicuruzwa bikubiyemo gusuzuma byihuse abarwayi ba Tuberculose, uburyo bwo kurwanya ibiyobyabwenge, kwandika igituntu cya Mycobacterium na NTM, hypercensivite abantu ba bagiteri-abantu babi, nibindi.
Gutahura Ibikoresho bya Tuberculose na MycobaCaCheia na MycobaCaCheia:
| Ingingo Oya | Izina ry'ibicuruzwa | Ibicuruzwa bigerageza ibicuruzwa | Ubwoko bw'icyitegererezo | Icyitegererezo |
| Hwts-3012 | Icyitegererezo kirega umukozi | Ikoreshwa mu kuvura imizigo ya Sputom, yabonye nimero ya mbere yandika, ibikoresho by'imashini ya Sutheng Ibikoresho 20230047. | sputum | |
| Hwts-ngs-p00021 | Amafaranga ahembwa ya Quentction Gutahura Igituntu cya Hypersensitie (Uburyo bwa Probe Gufata) | Idatera (amazi ya biopsy) uburyo bwa hypercensitivite kuri bagiteri-ibitugu bya bagiteri-bibi igituba igituntu nubwonko; Ingero z'abantu bakekwaho kuza kwandura igituntu cyangwa igituntu MycobaCemia bikurikirana cyane, kandi amakuru yo gutahura yo niba mycobaCacteria nyinshi cyangwa igituntu cya MycobaCaria cyangwa igituntu cyanduye ndetse n'amashusho yo kurwanya ibiyobyabwenge. zatanzwe. | Amaraso ya perifeli, amazi ya alveolar, hydrothorax na ascite, yibanze kuntoki, amazi yo mu bwami. | Igisekuru cya kabiri |
| Hwts-ngs-t001 | Mycobacterium Kwandika no Kurwanya Ibiyobyabwenge Ibiyobyabwenge (uburyo bushya bwo gufungura uburyo bwo gukurikiranwa) | MycobaCeum Wandike Ikizamini, harimo MTBC na 187 ntm;Kurwanya ibiyobyabwenge Kumenya igituntu cya Mycobacterium gikubiyemo ibiyobyabwenge 13 na 16 nyamukuru bituruka muri genes zo kurwanya ibiyobyabwenge. | Prutum, Fluid ya alveolar Lavage, hydrothorax na ascite, kwibanda ku cumbaro, amazi yo muri cerebrospinal. | Icya kabiri / icya gatatu gitandukanye |
Ibikurubikuru: Hwts-Ngs-TGS11 Mycobacterium Kwandika no Kurwanya ibiyobyabwenge Ibiyobyabwenge
Intangiriro y'ibicuruzwa
Ibicuruzwa bishingiye kumiti nyamukuru-ya mbere yumurongo wavuzwe mumabwiriza ya TB, Macrolide na Aminoglycosids zisanzwe zikoreshwa mumabwiriza ya NTM, hamwe nibibuga byo kurwanya ibiyobyabwenge bikubiyemo Itsinda rimwe ryibiyobyabwenge muri Ninde Mycobacterium Catation Catalog ihindagurika, kimwe nibindi bipimo byo kurwanya ibiyobyabwenge na guteganwa hakurikijwe iperereza n'imibare yo gutanga amanota menshi Kudava mu gihugu no mu mahanga.
Kwandika indangamuntu bishingiye ku mibanire ya NTM muri make mu nama ya NTM yatangajwe n'ikinyamakuru cy'Ubushinwa n'indwara z'ubuhumekero hamwe n'impuguke. Kwandika kwandikirwa kwandikirwa birashobora kwagura, urutonde kandi kikavuga ko NTM irenze 190.
Binyuze mu igamije tekinoroji ya PCR ya PCR, ingirabuzimafatizo za Mycobangium zarimo ibiyobyabwenge na byinshi PCR, na amplicon guhuza ibigo bigamije kumenyekana byabonetse. Ibicuruzwa byakoronya birashobora kubakwa mu gisekuru cya kabiri cyangwa igisekuru-cyisumbuye-yakoresheje ibisekuruza byakurikiranye, hamwe n'ibisekuru bya kabiri na gatatu bikurikirana ibishushanyo mbonera n'igisenge-bya gatatu bikurikirana kugirango babone amakuru yimbitse kugirango babone amakuru akurikirana. Mugereranije no mu ihinduka rizwi rikubiye mu bakundwa mu gitabo cyubatswe (harimo na mycobacterium catalose ihindagurira no guhuza ibiyobyabwenge), ihinduka ry'ibiyobyabwenge), ihinduka ry'ibiyobyabwenge byagenwe. Ihujwe na sputum yafunguye umubyiciro wo kuvura igisubizo cya macro & micro-ikizamini cya acide nkeya noclic mu buryo butaziguye mu buryo butaziguye ingero za clinical.
Intera yo kumenya ibicuruzwa
34Ibiyobyabwenge bifitanye isano n'ibiyobyabwenge18Kurwanya ibiyobyabwenge no6Ibiyobyabwenge by NTM byagaragaye, bitwikiriye297Imbuga zo kurwanya ibiyobyabwenge; Ubwoko icumi bwa mycobacterium igituntu kandi birenze190Ubwoko bwa NTM bwagaragaye.
Imbonerahamwe 1: Amakuru ya 18 + 6 6 + 190 + ntm
Inyungu y'ibicuruzwa
Ibisobanuro bikomeye byubuvuzi: Urugero rwa Sputum rushobora kugaragara mu buryo butaziguye umukozi wo kwizihiza nta muco.
Igikorwa cyo kugerageza kiroroshye: Icyiciro cya mbere cyibikorwa byongeweho byoroshye, kandi kubaka isomero birangiye mumasaha 3, bitezimbere imikorere.
Kwandika kwandikira ibiyobyabwenge no kurwanya ibiyobyabwenge: Gupfukirana imbuga zandika hamwe n'ibiyobyabwenge bya MTB na NTM, aribyo ingingo z'ingenzi zo gutahura mu ivuriro, kumenyekanisha ibiyobyabwenge, no gutanga porogaramu z'isesengura.
Guhuza: Guhuza ibicuruzwa, guhuza na Mainstream ILM na MGI / ONT.
Ibicuruzwa
| Kode y'ibicuruzwa | Izina ry'ibicuruzwa | Platifomu | ibisobanuro |
| Hwts-ngs-t001 | Mycobacterium Kwandika no Kurwanya ibiyobyabwenge Ibiyobyabwenge (Uburyo bwa AFLX BLOWLICE) | ONT, Illumina, MGI, Salus Pro | 16 / 96Rxn |
Igihe cyohereza: Jan-23-2024