Amakuru - Ibikoresho Byo Gupima Mpox (RDTs, NAATs na Sequencing)

Kuva muri Gicurasi 2022, abarwayi ba Mpox bagiye bagaragara mu bihugu byinshi bitari ibyanduye ku isi aho abantu bo mu gace runaka bandura.

Ku itariki ya 26 Kanama, Umuryango Mpuzamahanga wita ku Buzima (OMS) watangije gahunda mpuzamahangaGahunda yo Kwitegura no Gukemura Ibibazo mu Ngambaguhagarika icyorezo cyo kwanduzanya kwandura kwa MPOX hagati y’abantu binyuze mu bikorwa bihuriweho ku rwego rw’isi, akarere n’igihugu. Ibi bikurikira itangazo ry’ikibazo cy’ubuzima rusange gihangayikishije mpuzamahanga ryatanzwe n’Umuyobozi Mukuru wa OMS ku ya 14 Kanama.

Icyitonderwa ni uko icyorezo cya mpox kuri iyi nshuro gitandukanye n’icyo mu 2022, cyakwirakwiraga cyane cyane mu bagabo bakorana imibonano mpuzabitsina n’abagabo, kandi umubare w’impfu z’abanduye wari munsi ya 1%.

Ubwoko buherutse kugaragara bwa “Clade Ib”, bugizwe n’ubwoko bwa Clade I, bufite igipimo cy’impfu kiri hejuru. Ubu bwoko bushya bwatangiye gukwirakwira muri Repubulika Iharanira Demokarasi ya Kongo mu kwezi kwa Nzeri gushize, bwabanje gukwirakwira mu bakora umwuga w’uburaya, none ubu bwageze no mu yandi matsinda, aho abana ari bo bibasirwa cyane.

Muri raporo y’ukwezi gushize, Ishami rishinzwe kurwanya indwara ya kanseri muri Afurika (Africa CDC) ryatangaje ko icyorezo cya Mpox cyagaragaye mu bihugu 10 bya Afurika uyu mwaka, harimo na RDC, aho byagaragaye ko 96.3% by’abanduye bose muri Afurika uyu mwaka na 97% by’abapfuye. Ni ngombwa kumenya ko hafi 70% by’abanduye muri RDC ari abana bari munsi y’imyaka 15, kandi iri tsinda rigize 85% by’impfu muri icyo gihugu.

Mpox ni indwara ya zoonosis iterwa na virusi ya mpox, ikaba imara iminsi 5 kugeza kuri 21, cyane cyane iminsi 6 kugeza kuri 13. Uwanduye azagira ibimenyetso nk'umuriro, kuribwa umutwe no kubyimba imitsi, bigakurikirwa no kubyimba uduheri mu maso no mu bindi bice by'umubiri, bigahita bihinduka uduheri kandi bikamara icyumweru kimwe mbere yo gukurura. Iyi ndwara yandura kuva ibimenyetso bitangiye kugeza igihe uduheri duto duto tuvamo ubwandu.

Macro & Micro-Test itanga ibizamini byihuse, ibikoresho bya molekile n'ibisubizo byo gukurikirana virusi ya mpox, bifasha mu gusuzuma virusi ya mpox ku gihe, kugenzura inkomoko yayo, inkomoko yayo, ikwirakwizwa ryayo n'impinduka mu ngirabuzimafatizo:

Antigen ya virusi ya MonkeypoxAgakoresho ko Gutahura (Gupima indwara ziterwa n'ubudahangarwa bw'umubiri (immunochromatography))

Gufata ingero zoroshye (urugero rw'amazi y'ibihushi/umuhogo) kandi umusaruro wihuse mu minota 10-15;

Ubushobozi bwo kwihuta cyane hamwe na LoD ya 20pg/mL itwikiriye Clade I na II；

Indwara yihariye cyane idafite ingaruka ku mikorere ya virusi ya smallpox, varicella zoster virus, rubella virus, herpes simplex virus, nibindi.

OPA ya 96.4% ugereranije na NAATs;

Ikoreshwa ryagutse nko muri gasutamo, CDC, farumasi, amavuriro, ibitaro cyangwa mu ngo.

Agakoresho ko Gupima Umubiri w'Agakoko gatera IgM/IgG mu Gupima Umubiri w'Agakoko gatera IgM/IgG(Indwara ya immunochromatographhy)

Gukoresha byoroshye nta gikoresho gikoreshwa kandi umusaruro wihuse mu minota 10;

Ubushobozi bwo kwihuta cyane n'ubwihariye bukubiyemo Clade I na II ；

Igaragaza IgM na IgG kugira ngo imenye ibyiciro byo kwandura kwa mpox;

Gukoreshwa mu buryo burambuye nko muri gasutamo, CDC, farumasi, amavuriro, ibitaro cyangwa mu ngo;

Birakwiriye gupimwa ku rwego runini abantu bakeka ko banduye indwara ya mpox.

Agakoresho ko Gupima Aside Nucleic Virus ya Monkeypox (Enzymatic Probe Isothermal Amplification)

Ubushobozi bwo kuvura cyane hamwe na LoD ya kopi 200/mL hamwe na IC, bingana na PCR ya florescence;

Gukora byoroshye: Icyitegererezo cya Lysed cyongewe kuri lyophilized reagent tube kugira ngo gikoreshwe mu buryo butaziguye hakoreshejwe modules zigenga za Easy Amp System;

Ubwinshi budasanzwe budafite ingaruka ku buryo buhuriweho na virusi y'inkorora, virusi y'inkingo, virusi y'inkorora, virusi y'inkorora, virusi y'inkorora, virusi ya herpes simplex, virusi ya varicella-zoster, na genome y'umuntu, n'ibindi;

Gufata ingero byoroshye (umuti wa rash fluid/oropharyngeal swab) kandi bikagira ingaruka nziza vuba mu minota 5;

Imikorere myiza cyane mu buvuzi ijyanye na Clade I na II hamwe na PPA ya 100%, NPA ya 100%, OPA ya 100% na Kappa Value ya 1.000 ugereranije na Fluorescence PCR kit;

Ubwoko bwa Lyophilized busaba gutwara no kubika ubushyuhe bw'icyumba gusa butuma umuntu ashobora kubugera mu turere twose;

Uburyo bworoshye bwo gusuzuma indwara mu mavuriro, ibitaro, hamwe na Easy Amp kugira ngo imenyekane igihe cyose bikenewe;

Agakoresho ko Gupima Aside Nucleic Virus ya Monkeypox (Fluorescence PCR)

Gene ebyiri zigamije kwanduzanya cyane hamwe na LoD ya kopi 200/mL;

Gufata ingero zoroheje z'amazi y'ibihumyo, agapfukamunwa ko mu muhogo n'amaraso;

Gukora byoroshye: isesengura ryihuse ry’icyitegererezo hakoreshejwe reagent yo kurekura icyitegererezo kugira ngo yongerwe mu muyoboro w’ikirahure;

Gutahura vuba: igisubizo mu minota 40;

Uburinganire bwemezwa n'igenzura ry'imbere rigenzura inzira yose yo gushakisha;

Imikorere myiza cyane mu buvuzi ijyanye na Clade I na II hamwe na PPA ya 100%, NPA ya 99.40%, OPA ya 99.64% n'agaciro ka Kappa ka 0.9923 ugereranije n'uburyo bwo gukurikirana;

Ubwoko bwa Lyophilized busaba gutwara no kubika ubushyuhe bw'icyumba gusa butuma umuntu ashobora kubugera mu turere twose;

Ihuye na sisitemu za PCR za Fluorescence zisanzwe;

Uburyo bworoshye bwo kuvura ibitaro, CDC na laboratwari;

Kit yo Gupima Acid Nucleic Virus ya Orthopox Universal Type/Monkeypox Virus (Fluorescence PCR)

Gupima byuzuye: gupima virusi zose enye za orthopox zishobora kwanduza abantu ndetse n’izisanzwe (harimo na Clade I na II) mu isuzuma rimwe kugira ngo hirindwe ko zitaboneka;

Ubushobozi bwo kuvura indwara cyane hamwe na LoD ya kopi 200/mL;

Ubwinshi budasanzwe butagira ingaruka ku zindi ndwara zitera uduheri nka virusi ya herpes simplex, virusi ya varicella-zoster, na genome y'umuntu, n'ibindi;

Gukora byoroshye: isesengura ryihuse ry’icyitegererezo hakoreshejwe reagent yo kurekura icyitegererezo kugira ngo yongerwe kuri buffer y’ikirahure cy’umuyoboro umwe;

Kumenya vuba: kongera imbaraga byihuse ku buryo umusaruro uboneka mu minota 40;

Uburinganire bwemezwa n'igenzura ry'imbere rigenzura inzira yose yo gushakisha;

Ihuye na sisitemu za PCR za Fluorescence zisanzwe;

Uburyo bworoshye bwo kuvura ibitaro, CDC na laboratwari;

Indwara y'inkendeVirus TgutondekanyaNucleicAcideDgupimaKbyo (Fluorescence PCR)

Gusobanura icyarimwe Clade I na Clade II, ibyo bikaba ari ingenzi mu gusobanukirwa imiterere y’ibyorezo bya virusi, gukurikirana ikwirakwira ryayo, no gushyiraho ingamba zo kwirinda no kurwanya.

Ubushobozi bwo kuvura indwara cyane hamwe na LoD ya kopi 200/mL;

Gufata ingero zoroheje z'amazi y'ibihumyo, agahu k'umunwa n'amaraso;

Ubwinshi budasanzwe budafite uburyo bwo guhuza Clade I na II, izindi ndwara zitera uduheri nka virusi ya herpes simplex, virusi ya varicella-zoster, na genome y'umuntu, n'ibindi;

Gukora byoroshye: isesengura ryihuse ry’icyitegererezo hakoreshejwe reagent yo kurekura icyitegererezo kugira ngo yongerwe kuri buffer y’ikirahure cy’umuyoboro umwe;

Gutahura vuba: igisubizo mu minota 40;

Uburinganire bwemezwa n'igenzura ry'imbere rigenzura inzira yose yo gushakisha;

Ubwoko bwa Lyophilized busaba gutwara no kubika ubushyuhe bw'icyumba gusa butuma umuntu ashobora kubugera mu turere twose;

Ihuye na sisitemu za PCR za Fluorescence zisanzwe;

Uburyo bworoshye bwo kuvura ibitaro, CDC na laboratwari;

Jenome yose ya virusi y'inkendeGutahuraIpaki (Multi-PCR NGS)

Ikimenyetso gishya cyo gupima jeni yose ya Monkeypox Virus cyakozwe na Macro & Micro-Test ku bintu bitandukanye, hamwe na ONT nanopore sequencer, gishobora kubona jeni yose ya MPXV ifite ubwinshi butari munsi ya 98% mu masaha 8.

Byoroshye gukoresha: ikoranabuhanga ryo kongera ingufu mu ntambwe imwe, uruhererekane rwose rwa genome ya virus ya mpox rushobora kuboneka hakoreshejwe kongera ingufu mu ntera imwe;

Ifite ubwenge kandi ifite ukuri: Ipima ingero ziri hasi kugeza kuri 32CT, kandi uburyo bwo gupima amplicon nanopore bwa 600bp bushobora guhuza genome nziza cyane;

Kwihuta cyane: ONT ishobora kurangiza guteranya genome mu masaha 6-8;

Guhuza neza: hamwe na ONT, Qi Carbon, SALUS Pro, lllumina, MGI n'izindi 2 zisanzwe^ndna 3^rdabakurikirana ibisekuruza.

Ifite ubushobozi bwo kwihuta cyaneJenome yose ya virusi y'inkendeGutahuraIpaki-Illumina/MGI(Multi-PCR NGS)

Ku bijyanye n'umubare munini w'abantu 2 bariho^ndabahanga mu gupima imiterere y’ibinyabuzima ku isi yose, Macro & Micro-Test nayo yateguye ibikoresho byoroshye cyane bihuza n’abahanga mu gupima imiterere y’ibinyabuzima kugira ngo habeho uburyo bwo gupima imiterere y’ibinyabuzima mu buryo budakomeye;

Kongera ingufu neza: Impande 1448 z'igishushanyo mbonera cya amplicon ya 200bp cyuzuye cyane kugira ngo yongere ingufu kandi ikwirakwizwe neza;

Gukora byoroshye: Isomero rya Mpox virus llumina/MGI rishobora kuboneka binyuze mu kongera imbaraga mu masaha 4, hirindwa intambwe zikomeye zo kubaka isomero n'ikiguzi cy'ibikoresho bigabanya ubukana;

Ububasha bwo kugaragaza ibimenyetso: bugaragaza ingero ziri hasi kugeza kuri 35CT, bukirinda ibisubizo bitari byo byatewe no kwangirika kw'ibice cyangwa umubare muto w'amakopi;

Guhuza neza na 2 rusange^ndabakurikirana ibizamini nka lllumina, Salus Pro cyangwa MGI;Kugeza ubu, abarwayi barenga 400 bamaze kuvura.

Igihe cyo kohereza: Kanama-28-2024

Ibikoresho birambuye byo gupima Mpox (RDTs, NAATs na Sequencing)