Kuva muri Gicurasi 2022, indwara za mpox zagaragaye mu bihugu byinshi bitari ibyorezo ku isi byanduza abaturage.
Ku ya 26 Kanama, Ishami ry'Umuryango w'Abibumbye ryita ku Buzima (OMS) ryatangije isi yoseGahunda yo Kwitegura no Gusubizaguhagarika icyorezo cyanduza abantu kwanduza mpox binyuze mubikorwa bihuriweho nisi, uturere, nigihugu. Ibi bikurikira nyuma y’itangazwa ry’ubuzima rusange bw’ibibazo by’ubuzima mpuzamahanga n’umuyobozi mukuru wa OMS ku ya 14 Kanama.
Twabibutsa ko icyorezo cya mpox muri iki gihe gitandukanye n’icyo mu 2022, cyakwirakwiriye cyane mu bagabo baryamana n’abagabo, kandi impfu z’abanduye ntizari munsi ya 1%.
Ubwoko bwa vuba aha “Clade Ib”, butandukanye na Clade I, bufite umubare munini w'impfu. Iyi variant nshya yatangiye gukwirakwira muri DRC muri Nzeri ishize, mu ikubitiro mu bakora imibonano mpuzabitsina, none ikwira mu yandi matsinda, aho usanga abana bibasirwa cyane.
Afurika CDC muri raporo mu kwezi gushize yavuze ko icyorezo cya mpox cyagaragaye mu bihugu 10 bya Afurika muri uyu mwaka, harimo na DRC, kikaba cyaragaragaje ko 96.3% by'abantu bose banduye muri Afurika muri uyu mwaka na 97% by'impfu. Twabibutsa ko hafi 70% by’abanduye muri DRC ari abana bari munsi y’imyaka 15, kandi iri tsinda rikaba rifite 85% by’impfu mu gihugu.
Mpox ni zoonose iterwa na virusi ya mpox hamwe na incububasi yiminsi 5 kugeza 21, cyane cyane iminsi 6 kugeza 13. Umuntu wanduye azaba afite ibimenyetso nk'umuriro, kubabara umutwe ndetse no kubyimba lymph node, bikurikirwa no guhubuka mu maso no mu bindi bice by'umubiri, bigenda bikura bikavamo ibibyimba bikamara hafi icyumweru mbere yo gufatwa. Uru rubanza rwanduye kuva ibimenyetso byatangira kugeza ibisebe biguye bisanzwe.
Macro & Micro-Test iratanga ibizamini byihuse, ibikoresho bya molekile hamwe nibisubizo bikurikirana mugutahura virusi ya mpox, bifasha mugihe cyo gusuzuma virusi ya mpox mugihe, kugenzura inkomoko yayo, ibisekuru, kwanduza no gutandukana kwa genomic:
Monkeypox Virus AntigenKumenya (Immunochromatography)
Icyitegererezo cyoroshye (rash fluid / umuhogo sample) nibisubizo byihuse muminota 10-15;
Ubukangurambaga bukabije hamwe na LoD ya 20pg / mL itwikiriye Clade I & II ；
Umwihariko wo hejuru udafite cross-reactivite na virusi yibicurane, virusi ya varicella zoster, virusi ya rubella, virusi ya herpes simplex, nibindi.
OPA ya 96.4% ugereranije na NAATs;
Porogaramu nini nka gasutamo, CDC, farumasi, amavuriro, ibitaro cyangwa murugo.
Monkeypox-virusi IgM / IgG Antibody Detection Kit(Immunochromatographehy)
Igikorwa cyoroshye kitarimo ibikoresho nibisubizo byihuse muminota 10;
Ibyiyumvo bihanitse kandi byihariye bikubiyemo Clade I & II ；
Kumenya IgM na IgG guhitamo ibyiciro byanduye mpox;
Porogaramu nini nka gasutamo, CDC, farumasi, amavuriro, ibitaro cyangwa murugo;
Birakwiye mugupima nini mugukekwaho kwandura mpox.
Monkeypox Virus Nucleic Acide Detection Kit (Enzymatic Probe Isothermal Amplification)
Ubukangurambaga bukabije hamwe na LoD ya 200 Kopi / mL hamwe na IC, bingana na florescence PCR;
Igikorwa cyoroshye: Lysed sample yongewe kuri lyophilized reagent tube kugirango yongererwe kubisabwa byongerewe imbaraga na module yigenga ya Easy Amp Sisitemu;
Umwihariko cyane udafite reaction ya virusi yanduye, virusi yinkingo, virusi yinka, virusi ya mousepox, virusi ya herpes simplex, virusi ya varicella-zoster, na genome yabantu, nibindi.;
Icyitegererezo cyoroshye (rash fluid / oropharyngeal swab) nigisubizo cyiza cyihuse muminota 5;
Imikorere myiza yubuvuzi ikubiyemo Clade I & II hamwe na PPA ya 100%, NPA ya 100%, OPA ya 100% na Kappa Agaciro ka 1.000 ugereranije nibikoresho bya Fluorescence PCR;
Lyophilised verisiyo isaba gusa ubushyuhe bwicyumba cyo gutwara no kubika ituma bishoboka mu turere twose;
Ibintu byoroshye mumavuriro, ikigo nderabuzima, hamwe na Amp yoroshye yo kumenya kubisabwa;
Monkeypox Virus Nucleic Acide Detection Kit (Fluorescence PCR)
Gene ebyiri igamije kumva cyane hamwe na LoD ya 200 kopi / mL;
Icyitegererezo cyoroshye cya flux fluid, umuhogo swab na serumu;
Umwihariko cyane udafite reaction ya virusi yanduye, virusi yinkingo, virusi yinka, virusi ya mousepox, virusi ya herpes simplex, virusi ya varicella-zoster, na genome yabantu, nibindi.;
Igikorwa cyoroshye: byihuse lysis by sample irekura reagent kugirango yongerwe kumuyoboro wa reaction;
Kumenya byihuse: ibisubizo muminota 40;
Ukuri gukurikiranwa nubugenzuzi bwimbere bugenzura inzira zose zo gutahura;
Imikorere myiza yubuvuzi ikubiyemo Clade I & II hamwe na PPA ya 100%, NPA ya 99.40%, OPA ya 99,64% na Kappa Agaciro 0.9923 ugereranije nuburyo bukurikirana;
Lyophilised verisiyo isaba gusa ubushyuhe bwicyumba cyo gutwara no kubika ituma bishoboka mu turere twose;
Bihujwe na sisitemu nyamukuru ya Fluorescence PCR;
Ibintu byoroshye kubitaro, CDC na laboratoire;
Virusi ya Orthopox Ubwoko Bwose / Monkeypox Virus Nucleic Acide Detection Kit (Fluorescence PCR)
Ubwishingizi bwuzuye: bipimisha virusi zose 4 orthopox zishobora kwanduza abantu na mpox yiganje (Clade I&II irimo) mukizamini kimwe kugirango wirinde kubura;
Ubukangurambaga bukabije hamwe na LoD ya kopi 200 / mL;
Umwihariko udasanzwe udafite reaction ziterwa nizindi virusi zitera ibisebe nka virusi ya herpes simplex, virusi ya varicella-zoster, na genome yabantu, nibindi.;
Igikorwa cyoroshye: byihuse sample lysis by sample reagent kugirango yongerwe kumurongo umwe wa reaction reaction;
Kumenya byihuse: kwihuta byihuse hamwe nibisubizo muri min 40;
Ukuri gukurikiranwa nubugenzuzi bwimbere bugenzura inzira zose zo gutahura;
Bihujwe na sisitemu nyamukuru ya Fluorescence PCR;
Ibintu byoroshye kubitaro, CDC na laboratoire;
MonkeypoxVirus TypingNucleicAcidDgutoraK.ni (Fluorescence PCR)
Icyarimwe kigaragaza icyiciro cya mbere nicyiciro cya II, bifite akamaro kanini mugusobanukirwa ibiranga epidemiologique ya virusi, gukurikirana ikwirakwizwa ryayo, no gushyiraho ingamba zigamije gukumira no kugenzura.
Ubukangurambaga bukabije hamwe na LoD ya kopi 200 / mL;
Icyitegererezo cyoroshye cya rash fluid, oropharyngeal swab na serumu;
Umwihariko udasanzwe utagira umusaraba hagati ya Clade I na II, izindi virusi zitera ibisebe nka virusi ya herpes simplex, virusi ya varicella-zoster, na genome yabantu, nibindi.;
Igikorwa cyoroshye: byihuse sample lysis by sample reagent kugirango yongerwe kumurongo umwe wa reaction reaction;
Kumenya byihuse: ibisubizo muminota 40;
Ukuri gukurikiranwa nubugenzuzi bwimbere bugenzura inzira zose zo gutahura;
Lyophilised verisiyo isaba gusa ubushyuhe bwicyumba cyo gutwara no kubika ituma bishoboka mu turere twose;
Bihujwe na sisitemu nyamukuru ya Fluorescence PCR;
Ibintu byoroshye kubitaro, CDC na laboratoire;
Virusi ya Monkey Universal Genome YoseKumenyaKit (Multi-PCR NGS)
Virusi ya Monkeypox Virus Yakozwe na Genro Detection Kit yakozwe na Macro & Micro-Test kubintu bitandukanye, ifatanije na ONT nanopore ikurikirana, irashobora kubona MPXV ikurikirana ya genome yose hamwe ikwirakwizwa na 98% mugihe cyamasaha 8.
Byoroshye gukora: patenti yintambwe imwe yo kongera imbaraga, genome yose ikurikirana ya virusi ya mpox irashobora kuboneka hamwe na amplification imwe;
Ibyiyumvo kandi byukuri: itahura ingero ziri hasi ya 32CT, na 600bp amplicon nanopore ikurikiranye irashobora guhura na genome yo murwego rwohejuru;
Ultra-yihuta: ONT irashobora kurangiza inteko ya genome mumasaha 6-8;
Ubwuzuzanye bwagutse: hamwe na ONT, Qi Carbone, SALUS Pro, lllumina, MGI nibindi byingenzi 2ndna 3rdibisekuruza.
Birenze urugeroVirusi y'inguge Genome yoseKumenyaKit-Illumina / MGI(Multi-PCR NGS)
Kubyerekeranye numubare munini wa 2 uharindIbisekuruza bikurikirana ku isi hose, Macro & Micro-Test byateje imbere kandi ibikoresho byoroheje byifashishwa mu guhuza ibyiciro rusange kugira ngo bigere kuri virusi ya genome ikurikirana;
Kwiyongera neza: 1448 joriji ya 200bp amplicon ultra-dense primer igishushanyo mbonera cyiza cyo gukwirakwiza no gukwirakwiza kimwe;
Igikorwa cyoroshye: Isomero rya virusi ya Mpox llumina / MGI irashobora kuboneka binyuze mu byiciro bibiri byongerewe imbaraga mu masaha 4, ukirinda intambwe zo kubaka amasomero bigoye hamwe nigiciro cya reagent;
Ubukangurambaga bukabije: butahura ingero ziri hasi ya 35CT, birinda neza ibisubizo bibi biterwa no gutesha agaciro ibice cyangwa umubare muto wa kopi;
Ubwuzuzanye bwagutse hamwe ningenzi 2ndibisekuruza bikurikirana nka lllumina, Salus Pro cyangwa MGI;Kugeza ubu, indwara zirenga 400 zirarangiye.
Igihe cyo kohereza: Kanama-28-2024